Skip to main content
. 2019 Apr 30;10(32):3027–3039. doi: 10.18632/oncotarget.26903

Figure 3. Metastatic breast cancer cells reprogram MSCs toward an anti-inflammatory state.

Figure 3

(A) Experimental scheme to test the effect of conditioned medias from breast cancer cells or breast cancer cell conditioned media-educated MSCs on macrophage or monocyte gene expression profiles. (B–E) Gene expression heat maps and principal component analysis (PCA) plots from RAW246.7 cells in B and C, and THP1 cells in D and E for the six respective media treatment conditions outlined in A. Pseudo-colored heat maps were generated based upon qPCR relative quantification (RQ) values for each gene across all six samples normalized to the Mock conditioned media condition. One-way ANOVA was performed on each condition compared to Mock conditioned media and p-values < 0.05 were overlaid on the pseudo-colored heat map (ns = not significant, nd = not determined, n/a = not applicable). PCA plots were generated using Multiple Experiment Viewer (MeV) for all RQ data irrespective of statistical significance.