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. 2019 Apr 17;24(8):1510. doi: 10.3390/molecules24081510

Figure 1.

Figure 1

DHL reduced p38 MAPK, MK2 and Akt phosphorylation in LPS-stimulated RAW264.7 cells. (A) Chemical structure of DHL. RAW264.7 cells were pretreated with either 5 μmol/L DHL or 0.1% DMSO for 0.5 h before stimulation with LPS (100 ng/mL) for 0, 0.5, 1, 2, 4 and 8 h. (B) The protein expression levels of p-p38 MAPK, p38 MAPK, p-MK2, MK2, p-Akt and Akt were evaluated by western blotting. (C–E) Quantitative analysis of the ratios of p-p38/T-p38, p-MK2/MK2 and p-Akt/Akt normalized with β-actin was performed using Image J software. Data represents means ± SEM based on three independent experiments, * p < 0.05 and ** p < 0.01.