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. 2019 Apr 17;24(8):1510. doi: 10.3390/molecules24081510

Figure 2.

Figure 2

DHL inhibited NO production and iNOS expression in LPS-stimulated macrophages. (A) RAW264.7 cells were treated with DHL at 0, 3, 5, 10 and 30 μmol/L for 48 h, and cell viability was determined by MTT assay. (B,C) RAW264.7 cells were pretreated with 0, 3, 5, 10 and 30 μmol/L DHL for 30 mins followed by stimulation with LPS (100 ng/mL) for 24 h, the supernatant of culture medium was collected for NO detection, and the mRNA levels of iNOS were analyzed by quantitative real-time PCR. Lung macrophages were pretreated with 0, 3, 5, 10 and 30 μmol/L DHL for 30 min followed by stimulation with LPS (100 ng/mL) for 8 and 16 h, (D) The culture supernatants were collected and NO levels detected with a Griess reagent kit; (E) Cells were collected to isolate total RNA, and the mRNA levels of iNOS were analyzed by quantitative real-time PCR. The data represent mean ± SEM of three independent experiments, * p < 0.05 and ** p < 0.01.