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. 2019 Apr 17;24(8):1510. doi: 10.3390/molecules24081510

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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DHL attenuated LPS-induced acute lung injury in vivo. (A) Mice were subjected to an intratracheal injection of LPS (5 mg/kg) and subsequent intraperitoneal injection of solvent or DHL (5, 10 and 20 mg/kg respectively), (B) Histological analysis of lung tissue sections by H&E staining. The original magnification was 200×, and detailed images are shown in lower right corner. (C) Lung tissue injury was assessed by histological scores in all groups. (D) The total cell count in BALF after red blood cell lysis. (E) Measurement of the total protein concentration of BALF. (F) Determination of the myeloperoxidase (MPO) activity in lung homogenates. Data represent means ± SEM, n = 6–8, * p < 0.05 and ** p < 0.01.