Effects of topical cinnamaldehyde or systemic TRPA1 antagonism on wound severity. Wound severity was macroscopically evaluated. Representative photographs (a) and severity scores (b) of the wounds on days 4 and 7 post-skin excision. Animals received either sterile saline or P. aeruginosa ATCC 27853 following skin excision. Cinnamaldehyde (0.5 mg/mL; 30 µL, n = 6) or 2% DMSO in sterile saline (30 µL, n = 6) were topically applied once a day for over seven days. The TRPA1 antagonist HC-030031 (30 mg/kg, i.p., n = 6) or vehicle (8% DMSO in saline, i.p., n = 6) were administered to animals receiving cinnamaldehyde or not. * p < 0.05, differs from vehicle-treated non-infected mice; #
p < 0.05, differs from vehicle-treated P. aeruginosa infected mice; @
p < 0.05, differs from cinnamaldehyde-treated P. aeruginosa infected mice.