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. 2018 Nov 22;104(5):973–985. doi: 10.3324/haematol.2018.199661

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Figure 4. — Effects of AC-73 on cell growth, apoptosis and viability of acute myeloid leukemia (AML) cell lines and autophagy. (A) Dose response analysis of AC-73 treatment on leukemic cell growth, as compared to control cells (C). (B) Analysis of the effects of AC-73 treatment performed for 3 days (d3) on leukemic cell apoptosis, as compared to control leukemic cell (0). Total apoptosis by annexin V/PI (%) detected by using flow cytometric apoptotic assays is indicated. (C and D) Cell viability assays on leukemic cells treated: (C) with AC-73 used at different concentrations for 2 days; (D) at different times with 2.5 μM AC-73, as compared to control cells (day 0). (C and D) Viability is presented as percentage viable cell relative to control. (E) Western blot analysis of the autophagy related protein LC3 and its conversion from LC3-I to LC3-II form in leukemic cells treated with AC-73 used at different concentrations, as compared to control (−) cells. (F) Dose response analysis of AC-73 treatment on the autophagy flux in U937 and NB4 leukemic cells, as compared to control cells (0). (G) Induction of autophagy by AC-73 treatment (+) in several AML cell lines, as compared to control cells (−). (A-D, F and G) Mean±Standard Error of Mean is shown. *P<0.05; **P<0.01; ***P<0.001. ns: not significant. (E) One representative western blot experiment out of three is shown. Actin was used as an internal control.