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. Author manuscript; available in PMC: 2019 Nov 20.
Published in final edited form as: Biochemistry. 2018 Nov 5;57(46):6538–6550. doi: 10.1021/acs.biochem.8b00987

Figure 3.

Figure 3.

Nup358 binds to BicD2 with high affinity. (A) The ITC titration curve of BicD2-CTD with Nup358-RBD2 is shown, from which the affinity was determined to 0.5 ± 0.07 μM. (B) ITC titration curve of the BicD2-CTD with the Nup358-RBD2/RanGTP complex. The affinity was determined to be 3.8 ± 0.4 μM. Note that RanGTP is a negative regulator of the interaction that lowers the affinity of Nup358 toward BicD2 by a factor of 10.