Table 3A.
Calculation of concentrations of Nup358/BicD2 and Rab6/BicD2 complexes formed, as well as of the free reactants (BicD2free, Nup358free, Rab6free).
| Condition | Input parameters (nM) | BÌCD2free (nM) | Nup358/BicD2 (nM) | Rab6/BicD2 (nM) | Nup358free (nM) | Rab6free (nM) |
|---|---|---|---|---|---|---|
| 1. Standard Condition (SC)] | KD1a=500 | 15 | 1.2 | 0.3 | 39 | 267 |
| KD2b=12,600 | ||||||
| Nup358tot=40 | ||||||
| Rab6tot=268 | ||||||
| BicD2totc=16 | ||||||
| 2. RanGTP present | SC KD1=3,800 | 16 | 0.2 | 0.3 | 40 | 267 |
| 3. Nup358 absent | SC Nup358tot=0 | 16 | 0 | 0.3 | 0 | 267 |
| 4. Rab6 absent | SC Rab6tot=0 | 15 | 1.2 | 0 | 39 | 0 |
| 5. Rab6 absent | SC Rab6tot=0 KD1=3,800 | 16 | 0.2 | 0 | .40 | 0 |
a
KD1 is the dissociation equilibrium constant for binding of Nup358 to BicD2.
b
KD2 is the dissociation equilibrium constant for binding of Rab6GTP to BicD2.
c
the subscripttot denotes total cellular concentration.