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. 2019 May 14;10(3):e00266-19. doi: 10.1128/mBio.00266-19

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Copyright © 2019 Archambault et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — Both C. albicans and C. parapsilosis elicit macrophage recruitment. Transgenic mpeg1:GAL4/UAS:nfsB-mCherry zebrafish (red macrophages) were infected and imaged at 24 hpi. (A to C) Representative images of zebrafish swimbladders injected with the vehicle (A), C. parapsilosis (B), and C. albicans (C). Maximum projections of 16 slices (A) and 13 slices (B and C), with (left) and without (right) a single DIC z-slice, are shown. (D) Numbers of macrophages per fish in the swimbladder lumen. Data were pooled from 7 independent experiments. (E) Numbers of macrophages per fish involved in interactions with C. parapsilosis or C. albicans. (F) Percentages of interacting macrophages engaged in phagocytosis. (G) Numbers of macrophages per fish interacting with fungi. Numbers of macrophages scored for the vehicle, C. parapsilosis, and C. albicans were 137, 135, and 367, respectively. Statistics are described in Materials and Methods (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001; ns, not significant [P > 0.05]). Bars, 150 μm (A to C).