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. 2019 Apr 14;11(4):345. doi: 10.3390/v11040345

Figure 1.

Figure 1

The knock-down of RdR6 in N. benthamiana resulted in a taller phenotype. N. benthamiana plants were subjected to a knock-down assay using a virus-induced gene silencing (VIGS) technique in order to verify the role of the RdR6 mRNA in determining the plant’s phenotype. (A) At 18 days post-agroinfiltration (dpa), the plants exhibited phenotypic alterations. (B) At 28-dpa the plants exhibited noticeable differences in their heights. Total RNA extracted from systemic leaf samples from the agroinfiltrated plants was subjected to (C) an RNA gel blot and (D) an RT-qPCR assay in order to evaluate the down-regulation of RdR6 in N. benthamiana plants. The expression change is presented on a log2 scale. EV, plants agroinfiltrated with pTRV2-EV vector; pds, plants agroinfiltrated with pTRV2:PDS vector; rdr6, plants agroinfiltrated with pTRV2-RdR6 vector. Each experiment was performed at least three times with true biological replicates.