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. 2019 Jun;25(6):713–726. doi: 10.1261/rna.069625.118

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© 2019 Wiatrek et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — The immune response induced by dsRNA from Trp53 MEFs is mediated primarily via the MDA5 receptor. (A) Immunoblot showing the expression of MDA5 and RIG-I receptors after transfection of Adar1;Trp53 MEFs with dsRNA from Trp53 MEFs. Prior to transfection, RIG-I was knocked down in the cells with esiRNA. Data are the mean ± SD of three independent experiments and quantified using Image J software. (B) Immunoblot showing the expression of MDA5 and RIG-I receptors after transfection of Adar1;Trp53 MEFs with dsRNA from Trp53 MEFs. Prior to transfection, MDA5 was knocked down in the cells with esiRNA. Data are mean ± SD of three independent experiments and quantified using Image J software. In cases where bands were not quantifiable for all biological replicates (e.g., MDA5 was not visible upon MDA5 knockdown), error bars were not included in the quantification diagram.