Figure 4.

The impact of rearing regime and inoculation with E. cloacae gut bacteria on fitness of self-limiting males in mating competition experiments. Here, fitness is measured from the proportion of transgenic fluorescent male SL progeny in cages with 10:1 SL: Wild type release ratio and 120 insects per cage. In the aseptic regime, we scored a minimum of 100 progeny per mating cage; in the standard treatment, 200–400 progeny were scored per cage. Asterisks indicate significantly different treatment contrasts (** p < 0.01, **** p < 0.0001).