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. 2019 Apr 17;8(4):360. doi: 10.3390/cells8040360

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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IGFBP2 is a direct target of miR-34b-5p. (A) The miR-34b-5p binding site in the IGFBP2 mRNA 3′UTR. The seed sequence and mutant sequence in miR-34b-5p were highlighted in blue and green, respectively. (B) The potential miR-34b-5p targeting site in IGFBP2 mRNA 3′UTR was analyzed by RNAhybrid software. (C,D) The mRNA and protein level of IGFBP2 in CPM cells after being transfected with miR-34b-5p mimic or inhibitor. (E) The dual-luciferase reporter assay was performed by co-transfecting a wild-type or mutant IGFBP2 3′UTR with miR-34b-5p mimic or mimic NC in DF-1 cells. Results are shown as mean ± S.E.M. and the data are representative of at least three independent assays. Independent sample t-test was used to analyze the statistical differences between groups. (* P < 0.05).