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. 2019 Apr 24;8(4):372. doi: 10.3390/cells8040372

Figure 6.

Figure 6

Leptin promotes white adipocyte browning by inhibiting the Hh signaling pathway. (A) Oil red O staining. (B–E) RT-qPCR analysis of gene expression related to thermogenic genes, mitochondrial genes, mitochondrial DNA content, and the key transcription factor Glis of the Hh signaling pathway. (F) Western blotting analysis of the expression of the key transcription factor Glis of the Hh signaling pathway and browning thermogenic gene expression. (G) The resulting quantity protein level. Scale bar = 20 μm, * p < 0.05; ** p < 0.01.