Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (S. pombe) |
pom1-yomNeon
Green::hphR |
This Paper | JM4496 | Integration through PCR product transformation |
| Strain, strain background (S. pombe) |
pom1-3HA::hphR
ura4-D18 leu1-32 h+ |
PN948 | JM797 | Paul Nurse Lab |
| Strain, strain background (S. pombe) |
pom1∆::natR h- | Lab Stock | JM966 | |
| Strain, strain background (S. pombe) |
pom1-m2-yomNeon
Green::hphR ura4-D18 leu1-32 h- |
This Paper | JM5412 | Integration through PCR product transformation |
| Strain, strain background (S. pombe) |
pom1-3HA::hphR
tea1∆::ura4 + ura4-D18 |
This Paper | JM5414 | Progeny from cross between JM797 and JM219 |
| Strain, strain background (S. pombe) |
pom1-3HA::hphR
tea4∆::kanMX6 |
This Paper | JM5415 | Progeny from cross between JM797 and J M2256 |
| Strain, strain background (S. pombe) |
pom1-yomNeonGreen::hphR
tea1∆::kanMX6 |
This Paper | JM4792 | Progeny from cross between JM4496 and JM838 |
| Strain, strain background (S. pombe) |
pom1-yomNeonGreen::hphR
tea4∆::kanMX6 |
This Paper | JM4791 | Progeny from cross between JM4496 and JM218 |
| Strain, strain background (S. pombe) |
pom1-yomNeonGreen::hphR
cdr2-tagRFP-t::hphR |
This Paper | JM5373 | Progeny from cross between JM4699 and JM4160 |
| Strain, strain background (S. pombe) |
cdr2-yomNeonGreen::hphR h- | Lab Stock | JM4493 | |
| Strain, strain background (S. pombe) |
wee1-yomNeon
Green::hphR cdr2∆::natR |
Lab Stock | JM4525 | |
| Strain, strain background (S. pombe) |
cdr2-yomNeonGreen::hphR
pom1-tdTomato::natR |
This Paper | JM5135 | Progeny from cross between JM935 and JM4493 |
| Strain, strain background (S. pombe) |
cdr2-mEGFP::kanMX6
ura4-D18 leu1-32 ade6-m210 h+ |
Lab Stock | JM346 | |
| Strain, strain background (S. pombe) |
cdr2-yomNeon
Green::hphR pom1∆::natR |
This Paper | JM5359 | Progeny from cross between JM5238 and JM966 |
| Strain, strain background (S. pombe) |
wee1-yomNeon
Green::hphR h- |
Lab Stock | JM4495 | |
| Strain, strain background (S. pombe) |
wee1-yomNeon
Green::hphR pom1∆::kanMX6 |
Lab Stock | JM4527 | |
| Strain, strain background (S. pombe) |
972 h- | PN1 | JM366 | Paul Nurse lab |
| Strain, strain background (S. pombe) |
cdr2∆::natR ura4-D18
leu1-32 h+ |
Lab Stock | JM600 | |
| Strain, strain background (S. pombe) |
wee1-yomNeon
Green::hphR cdr2(E177A) |
Lab Stock | JM4578 | |
| Recombinant DNA reagent |
pFA6a-yomNeon Green::hphR |
Lab Stock | pJM1344 | |
| Recombinant DNA reagent |
pFA6a-tdTomato::natR | Lab Stock | pJM248 | |
| Recombinant DNA reagent |
pFA6a-yomTagRFP-T::hphR | Lab Stock | pJM1221 | Derived from Addgene Plasmid 44842 |
| Recombinant DNA reagent |
pFA6a-mEGFP::kanMX6 | Lab Stock | pJM228 | |
| Recombinant DNA reagent |
pFA6a-3HA::hphR | Lab Stock | pJM216 | |
| Antibody | anti-HA (mouse monoclonal) | Covance | MMS-101R | WB (1:3000) |
| Antibody | anti-GST (rabbit polycolonal) | Covance | custom | WB (1:3000) |
| Chemical compound, drug |
1,2-dioleoyl-sn-glycero-3- phospho-L-serine (sodium salt) |
Avanti Polar Lipids | 840035C | 18:1 DOPS or ‘PS’ |
| Chemical compound, drug |
1,2-dioleoyl-sn-glycero-3- phosphoethanolamine-N -(lissamine rhodamine B sulfonyl) (ammonium salt) |
Avanti Polar Lipids | 810150C | 18:1 Liss Rhod PE |
| Chemical compound, drug |
1,2-dioleoyl-sn- glycero-3-phosphocholine |
Avanti Polar Lipids | 850375C | 18:1 (Δ9-Cis) PC (DOPC) or ‘PC’ |