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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 2019 May 6;116(20):10184–10185. doi: 10.1073/pnas.1906635116

Correction for Helbert et al., Discovery of novel carbohydrate-active enzymes through the rational exploration of the protein sequences space

PMCID: PMC6525495  PMID: 31061113

BIOCHEMISTRY Correction for “Discovery of novel carbohydrate-active enzymes through the rational exploration of the protein sequences space,” by William Helbert, Laurent Poulet, Sophie Drouillard, Sophie Mathieu, Mélanie Loiodice, Marie Couturier, Vincent Lombard, Nicolas Terrapon, Jeremy Turchetto, Renaud Vincentelli, and Bernard Henrissat, which was first published March 8, 2019; 10.1073/pnas.1815791116 (Proc Natl Acad Sci USA 116:6063–6068).

The authors wish to note the following: “We have found small errors in Table 2 and Table 3; these errors do not change the main conclusions of the paper, except that one additional novel family is now listed. A protein was wrongly assigned to GH42_dist in the original Table 2. This protein and its orthologs actually form a family completely different from GH42. The orientation of the glycosidic bond cleaved by this protein is different from that of bona fide GH42 proteins. The protein in question is now removed from corrected Table 2 and is listed as GH164 in corrected Table 3. Additionally, a typo and an incorrect GenBank accession number were also corrected in Table 3. The corrected paper reports on an additional novel GH family of proteins. We apologize for the errors.” The corrected Table 2 and Table 3 appear below.

Table 2.

Activity of enzymes distantly related to the described GH or PL (GH/PLxx_dist) families

Distant CAZy family GenBank accession no. Substrate Organism
GH2_dist WP_029427454.1 pNP-β-d-xylopyranoside (new) Bacteroides cellulosilyticus WH2
GH2_dist WP_029428707.1 Tamarind gum (new) Bacteroides cellulosilyticus WH2
GH2_dist WP_029428765.1 pNP-β-d-glucuronide Bacteroides cellulosilyticus WH2
GH2_dist WP_018628801.1 pNP-β-d-glucuronide Niabella aurantiaca DSM 17617
GH3_dist AJG33435.1 pNP-β-d-NAc-glucopyranoside Rickettsia rickettsii str. R
GH5_dist ZP_06241352.1 pNP-β-d-mannopyranoside Victivallis vadensis ATCC BAA-548
GH10_dist EMS72420.1 pNP-β-d-xylopyranoside (weak) Clostridium termitidis CT1112
GH16_dist ZP_02063674.1 pNP-β-d-glucopyranoside (new) Bacteroides ovatus ATCC 8483
GH20_dist AEV99795.1 pNP-β-d-NAc-6Sulf-glucopyranoside Niastella koreensis GR20-10
GH20_dist AHF94523.1 pNP-β-d-NAc-glucopyranoside Opitutaceae bacterium TAV5
GH31_dist EIY61740.1 pNP-α-d-galactopyranoside Bacteroides salyersiae CL02T12C01
GH36_dist EIY66649.1 pNP-α-d-galactopyranoside Bacteroides salyersiae CL02T12C01
GH36_dist ACS99969.1 pNP-α-d-galactopyranoside Paenibacillus sp. JDR-2
GH36_dist ACS99975.1 pNP-α-d-galactopyranoside Paenibacillus sp. JDR-2
GH36_dist ZP_06242255.1 pNP-α-d-galactopyranoside Victivallis vadensis ATCC BAA-548
GH49_dist EDY96541.1 Chaetomorpha sp. CWP (new) Bacteroides plebeius DSM 17135
GH49_dist EDY96565.1 Chaetomorpha sp. CWP (new) Bacteroides plebeius DSM 17135
GH51_dist WP_084555785.1 Lichenan (new) Alkaliflexus imshenetskii DSM 15055
GH76_dist ADO68190.1 pNP-α-d-maltopyranoside (new) Stigmatella aurantiaca DW4/3–1
GH106_dist WP_018627535.1 pNP-α-l-rhamnopyranoside Niabella aurantiaca DSM 17617
GH106_dist ACT02314.1 pNP-α-l-rhamnopyranoside Paenibacillus sp. JDR-2
GH117_dist WP_010134686.1 pNP-β-d-galactofuranoside Flavobacteriaceae bacterium S85

This set encompasses enzymes that fall outside of established subfamilies or that are only distantly related to biochemically characterized enzymes. “New” designates novel specificity in the family. CWP, cell wall polysaccharide.

Table 3.

Substrate specificity of new CAZy families

New family GenBank accession no. Substrate Activity Organism
GH147 WP_029428318.1 β-galactan Endo-β-(1,4)-galactanase Bacteroides cellulosilyticus WH2
GH147 EFI37897.1 β-galactan Endo-β-(1,4)-galactanase Bacteroides sp. 3_1_23
GH148 AGN79260.1 Konjac glucomannan Endo-β-(1,4)-glucosidase Pseudomonas putida H8234
GH148 ACR13278.1 Konjac glucomannan Endo-β-(1,4)-glucosidase Teredinibacter turnerae T7901
GH157 WP_029429093.1 CM-curdlan Endo-β-(1,3)-glucanase Bacteroides cellulosilyticus WH2
GH158 ZP_06243608.1 CM-curdlan Endo-β-(1,3)-glucanase Victivallis vadensis ATCC BAA-548
GH159 WP_007210837.1 pNP-β-d-galactofuranoside β-galactofuranosidase Bacteroides cellulosilyticus WH2
GH160 AEI51087.1 EPS Nostoc commune (new) Endo-β-(1,4)-galactosidase Runella slithyformis DSM 19594
GH164 EIY59668.1 PNP-α-d-mannopyranoside α-mannosidase Bacteroides salyersiae CL02T12C01
PL30 WP_029426181.1 Hyaluronan Endo-hyaluronan lyase Bacteroides cellulosilyticus WH2
PL31 ABD82242.1 β-glucuronan Endo-β-(1,4)-glucuronan lyase Saccharophagus degradans 2–40
PL31 AGF62897.1 β-glucuronan Endo-β-(1,4)-glucuronan lyase Streptomyces hygroscopicus subsp. jinggangensis TL01
PL32 EIY62149.1 β-mannuronan Endo-mannuronan lyase Bacteroides salyersiae CL02T12C01
PL33 ALJ61728.1 Hyaluronan Endo-hyaluronan lyase Bacteroides cellulosilyticus WH2
PL33 AHF90976.1 Gellan (new) Endo-gellan lyase Opitutaceae bacterium TAV5
PL33 AHF90672.1 Chondroitin sulfate Endo-chondroitin sulfate lyase Opitutaceae bacterium TAV5
PL33 AHF90411.1 Gellan (new) Endo-gellan lyase Opitutaceae bacterium TAV5
PL34 AHF91913.1 Alginate Endo-alginate lyase Opitutaceae bacterium TAV5
PL35 ZP_06241351.1 Chondroitin Endo-chondroitin lyase Victivallis vadensis ATCC BAA-548
PL36 WP_026728881.1 β-mannuronan Endo-mannuronan lyase Flavobacterium denitrificans DSM 15936

The substrate and the modality of substrate degradation are specified. “New” designates novel specificity not reported previously. Note that families GH147 and 148 were reported by other groups during the course of our work (30, 31). CM, carboxymethyl.


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