BIOCHEMISTRY Correction for “Discovery of novel carbohydrate-active enzymes through the rational exploration of the protein sequences space,” by William Helbert, Laurent Poulet, Sophie Drouillard, Sophie Mathieu, Mélanie Loiodice, Marie Couturier, Vincent Lombard, Nicolas Terrapon, Jeremy Turchetto, Renaud Vincentelli, and Bernard Henrissat, which was first published March 8, 2019; 10.1073/pnas.1815791116 (Proc Natl Acad Sci USA 116:6063–6068).
The authors wish to note the following: “We have found small errors in Table 2 and Table 3; these errors do not change the main conclusions of the paper, except that one additional novel family is now listed. A protein was wrongly assigned to GH42_dist in the original Table 2. This protein and its orthologs actually form a family completely different from GH42. The orientation of the glycosidic bond cleaved by this protein is different from that of bona fide GH42 proteins. The protein in question is now removed from corrected Table 2 and is listed as GH164 in corrected Table 3. Additionally, a typo and an incorrect GenBank accession number were also corrected in Table 3. The corrected paper reports on an additional novel GH family of proteins. We apologize for the errors.” The corrected Table 2 and Table 3 appear below.
Table 2.
Activity of enzymes distantly related to the described GH or PL (GH/PLxx_dist) families
| Distant CAZy family | GenBank accession no. | Substrate | Organism |
| GH2_dist | WP_029427454.1 | pNP-β-d-xylopyranoside (new) | Bacteroides cellulosilyticus WH2 |
| GH2_dist | WP_029428707.1 | Tamarind gum (new) | Bacteroides cellulosilyticus WH2 |
| GH2_dist | WP_029428765.1 | pNP-β-d-glucuronide | Bacteroides cellulosilyticus WH2 |
| GH2_dist | WP_018628801.1 | pNP-β-d-glucuronide | Niabella aurantiaca DSM 17617 |
| GH3_dist | AJG33435.1 | pNP-β-d-NAc-glucopyranoside | Rickettsia rickettsii str. R |
| GH5_dist | ZP_06241352.1 | pNP-β-d-mannopyranoside | Victivallis vadensis ATCC BAA-548 |
| GH10_dist | EMS72420.1 | pNP-β-d-xylopyranoside (weak) | Clostridium termitidis CT1112 |
| GH16_dist | ZP_02063674.1 | pNP-β-d-glucopyranoside (new) | Bacteroides ovatus ATCC 8483 |
| GH20_dist | AEV99795.1 | pNP-β-d-NAc-6Sulf-glucopyranoside | Niastella koreensis GR20-10 |
| GH20_dist | AHF94523.1 | pNP-β-d-NAc-glucopyranoside | Opitutaceae bacterium TAV5 |
| GH31_dist | EIY61740.1 | pNP-α-d-galactopyranoside | Bacteroides salyersiae CL02T12C01 |
| GH36_dist | EIY66649.1 | pNP-α-d-galactopyranoside | Bacteroides salyersiae CL02T12C01 |
| GH36_dist | ACS99969.1 | pNP-α-d-galactopyranoside | Paenibacillus sp. JDR-2 |
| GH36_dist | ACS99975.1 | pNP-α-d-galactopyranoside | Paenibacillus sp. JDR-2 |
| GH36_dist | ZP_06242255.1 | pNP-α-d-galactopyranoside | Victivallis vadensis ATCC BAA-548 |
| GH49_dist | EDY96541.1 | Chaetomorpha sp. CWP (new) | Bacteroides plebeius DSM 17135 |
| GH49_dist | EDY96565.1 | Chaetomorpha sp. CWP (new) | Bacteroides plebeius DSM 17135 |
| GH51_dist | WP_084555785.1 | Lichenan (new) | Alkaliflexus imshenetskii DSM 15055 |
| GH76_dist | ADO68190.1 | pNP-α-d-maltopyranoside (new) | Stigmatella aurantiaca DW4/3–1 |
| GH106_dist | WP_018627535.1 | pNP-α-l-rhamnopyranoside | Niabella aurantiaca DSM 17617 |
| GH106_dist | ACT02314.1 | pNP-α-l-rhamnopyranoside | Paenibacillus sp. JDR-2 |
| GH117_dist | WP_010134686.1 | pNP-β-d-galactofuranoside | Flavobacteriaceae bacterium S85 |
This set encompasses enzymes that fall outside of established subfamilies or that are only distantly related to biochemically characterized enzymes. “New” designates novel specificity in the family. CWP, cell wall polysaccharide.
Table 3.
Substrate specificity of new CAZy families
| New family | GenBank accession no. | Substrate | Activity | Organism |
| GH147 | WP_029428318.1 | β-galactan | Endo-β-(1,4)-galactanase | Bacteroides cellulosilyticus WH2 |
| GH147 | EFI37897.1 | β-galactan | Endo-β-(1,4)-galactanase | Bacteroides sp. 3_1_23 |
| GH148 | AGN79260.1 | Konjac glucomannan | Endo-β-(1,4)-glucosidase | Pseudomonas putida H8234 |
| GH148 | ACR13278.1 | Konjac glucomannan | Endo-β-(1,4)-glucosidase | Teredinibacter turnerae T7901 |
| GH157 | WP_029429093.1 | CM-curdlan | Endo-β-(1,3)-glucanase | Bacteroides cellulosilyticus WH2 |
| GH158 | ZP_06243608.1 | CM-curdlan | Endo-β-(1,3)-glucanase | Victivallis vadensis ATCC BAA-548 |
| GH159 | WP_007210837.1 | pNP-β-d-galactofuranoside | β-galactofuranosidase | Bacteroides cellulosilyticus WH2 |
| GH160 | AEI51087.1 | EPS Nostoc commune (new) | Endo-β-(1,4)-galactosidase | Runella slithyformis DSM 19594 |
| GH164 | EIY59668.1 | PNP-α-d-mannopyranoside | α-mannosidase | Bacteroides salyersiae CL02T12C01 |
| PL30 | WP_029426181.1 | Hyaluronan | Endo-hyaluronan lyase | Bacteroides cellulosilyticus WH2 |
| PL31 | ABD82242.1 | β-glucuronan | Endo-β-(1,4)-glucuronan lyase | Saccharophagus degradans 2–40 |
| PL31 | AGF62897.1 | β-glucuronan | Endo-β-(1,4)-glucuronan lyase | Streptomyces hygroscopicus subsp. jinggangensis TL01 |
| PL32 | EIY62149.1 | β-mannuronan | Endo-mannuronan lyase | Bacteroides salyersiae CL02T12C01 |
| PL33 | ALJ61728.1 | Hyaluronan | Endo-hyaluronan lyase | Bacteroides cellulosilyticus WH2 |
| PL33 | AHF90976.1 | Gellan (new) | Endo-gellan lyase | Opitutaceae bacterium TAV5 |
| PL33 | AHF90672.1 | Chondroitin sulfate | Endo-chondroitin sulfate lyase | Opitutaceae bacterium TAV5 |
| PL33 | AHF90411.1 | Gellan (new) | Endo-gellan lyase | Opitutaceae bacterium TAV5 |
| PL34 | AHF91913.1 | Alginate | Endo-alginate lyase | Opitutaceae bacterium TAV5 |
| PL35 | ZP_06241351.1 | Chondroitin | Endo-chondroitin lyase | Victivallis vadensis ATCC BAA-548 |
| PL36 | WP_026728881.1 | β-mannuronan | Endo-mannuronan lyase | Flavobacterium denitrificans DSM 15936 |
The substrate and the modality of substrate degradation are specified. “New” designates novel specificity not reported previously. Note that families GH147 and 148 were reported by other groups during the course of our work (30, 31). CM, carboxymethyl.