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. 2019 May 1;146(9):dev174367. doi: 10.1242/dev.174367

Fig. 4.

Fig. 4.

Paxillinfl/flcre mammary epithelial cells are not able to form polarized acini in 3D matrigel culture. (A) Schematic of cells forming polarized acini. (B) Six-day-old acini stained for acetylated-tubulin and the Golgi marker GM130. Scale bar: 5 μm. (C) Six-day-old acini stained for Rab11. Scale bar: 5 μm. (D) Six-day-old acini stained for MyoVb and E-cadherin. Scale bar: 5 μm. (E) Quantification of cells forming polarized acini (more than 90% of the cells with apical Golgi), n=3 (total of 36-165 acini per genotype). (F) Quantification of acini with apical Rab11 distribution, n=3. (G) Quantification of acini with apical MyoVb distribution, n=3. (H) Six-day-old acini stained for F-actin. Scale bar: 5 μm. (I) Two-day-old acini stained for F-actin. Scale bar: 2 μm. (J) Quantification of acini with basal F-actin-containing blebs, n=3 (total of 26-74 acini per genotype). Student's t-test was performed. Data are mean±s.e.m. ns, not significant; **P<0.01, ***P<0.001.