Figure 8.
Mopex13 and Mopex14 are required in appressorial development and host penetration. The conidia of ∆mopex13, ∆mopex14 and wild type harvested from 9-day-cultured CM plates and suspended at 1 × 105 conidia/ml were incubated to allow germination and appressorial formation. (a&b) The conidial germination rates and appressorial formation rates at sequential time points were calculated and statistically compared. (c) Microscopy of the appressorial development of the strains. (d&e) Incipient-cytorrhysis was performed to compare the appressorial turgor genesis in the 24-h appressoria of strains using glycerol or PEG8000 as the external solute. Means and standard errors were calculated from three independent replicates (at least 100 conidia each). (f) The relative transcription of MoPEX13 and MoPEX14 during appressorial development was examined by qRT-PCR using the tubulin gene as a reference. (g) Inoculation of cuticle-removed (wound) barley leaves with 1 × 105 conidia/ml conidial suspensions. The mutants partially regained the ability to cause lesions on wounded leaves. (h) Infectious structure on inoculated intact and wounded barley leaves.