Skip to main content
. 2019 Mar 28;10(1):234–247. doi: 10.1080/21505594.2019.1596505

Figure 5.

Figure 5.

Conserved residues related to thiamine pyrophosphate transport in TptA are required for adaptation to iron-depleted conditions. (a) Schematic view of the TptA mutation sites. (b) Quantitative total conidial production for the indicated strains on MM in the presence or absence of 30 μM ThPP. (c) Quantitation of total conidial production for the indicated strains on MM with or without 50 μM BPS. 2 × 104 conidia of the indicated strains were cultured at 37°C for 48 h. (d) Relative RNA expression of hapX in tptAK255A and wild-type A. fumigatus under MM and MM-Fe broth at 37°C for 24 h. (e) TAFC production in tptAK255A and wild-type A. fumigatus in MM-Fe broth. TAFC production of the wild-type was used as a reference. Data are presented as the means ± SD from three independent replicates. “*”, “**”, “***” or “****” represent significant differences at P < 0.05, P < 0.01, P < 0.001 and P < 0.0001, respectively, according to t-test.