Figure 1.

Schematic diagram of CRISPR/Cas9-mediated site-specific integration of exogenous gene in the genome. (a) An illustration of the target sites of the designed sgRNAs in the genome. (b) Analysis of the efficacy of designed sgRNAs by T7E1 assay. Red arrows indicate the expected positions of the DNA bands cleaved by T7E1. (c) Strategy of CRISPR/Cas9-mediated site-specific integration of the exogenous gene in the genome. Red arrows indicate the location of primers for genotyping the cell line.