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. 2019 May 21;10(3):e00273-19. doi: 10.1128/mBio.00273-19

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Copyright © 2019 Hurst-Hess et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 1 — Deletion of σ^B confers RIF sensitivity in M. smegmatis (Msm), M. abscessus (Mab), and M. tuberculosis (Mtb). (a to c) Tenfold serial dilutions of M. smegmatis mc²155, M. abscessus ATCC 19977, M. tuberculosis mc²7000, and their respective ΔsigB and complemented strains were grown to an A₆₀₀ of 0.7 and spotted on Middlebrook 7H10 ADC or OADC containing the indicated concentrations of RIF. Deletion of sigB results in RIF sensitivity in all three strains. The mutant phenotype can be complemented by constitutive expression of the respective sigB gene.