FIG 5.

HPLC-based detection of ethanolamine in the supernatant from the 24-h and 96-h cultures of the M145 parental strain and ΔglnA4 mutant. Both strains were grown in defined Evans medium supplemented with ethanolamine (25 mM) as the sole N source. The HPLC chromatogram data are presented as follows: A, ethanolamine standard; B and C, detection of ethanolamine remaining in the supernatant of the M145 culture after 24 h (B) and 96 h (C) of growth; D and E, detection of ethanolamine remaining in the supernatant of the ΔglnA4 mutant culture after 24 h (D) and 96 h (E). The results indicate that only the S. coelicolor M145 parental strain was able to utilize more than one-third of ethanolamine from the medium after 96 h of incubation. mAU, milli-absorbance units.