Skip to main content
. 2019 May 13;8:e41586. doi: 10.7554/eLife.41586

Figure 6. Genetic deletion of Syn II reduces the heterogeneity of ultrastructural profiles of presynaptic terminals at GC-MLI synapses.

Figure 6.

(A) Representative micrographs of GC-MLI synapses captured in the upper part of the molecular layer of cerebellar parasagittal sections from WT and Syn II KO mice. (B) Upper panels, Cumulative distribution (left panel) and mean values of the number of docked SVs at GC-MLI synapses from WT and Syn II KO mice (black line/bar and red line/bar respectively). Lower panels, similar representations for the active zone length. Absence of Syn II reduced significantly the number of docked synaptic vesicles and the length of the active zone (mean number of docked synaptic vesicles: WT 6.83 ± 0.35, n = 108; Syn II KO 5.36 ± 0.28, n = 81, mean length of active zone: WT 521.3 nm ± 15.2, n = 108; Syn II KO 432.2 nm ± 13.2, n = 81). (C) Scatter plot of the number of docked synaptic vesicles (SVs) versus the active zone (AZ) length from dataset obtained in B. Genetic deletion of Syn II led to a specific loss of GC bouton endowed with both a long active zone (>800 nm) and high number of docked synaptic vesicles (>15 synaptic vesicles).