Abstract
Background
Elevated low-density lipoprotein cholesterol (LDL-C) levels in childhood have recently been found to be the strongest predictive risk factor for coronary artery disease in adulthood. There is an increased level of LDL-C in children and adolescents with short stature. However, the underlying factors associated with increased LDL-C levels in children and adolescents with short stature are unknown. In addition, the insulin-like growth factor 1 (IGF-1) level in the short-stature population is usually below the normal reference range. The aim of this study was to investigate the relationship between IGF-1 standard deviation score (IGF-1 SDS) and LDL-C level in children and adolescents with short stature.
Methods
A cross-sectional study was conducted in a single centre of China, 557 short-stature children and adolescents whose height SDS was lower than − 2 SD after adjustment for age and gender were included. The related clinical and laboratory examinations, including anthropometric parameters, lipid profiles, IGF-1 levels and the levels of other cofactors, were assessed in all participants.
Results
The univariate analysis results showed a significant negative correlation between IGF-1 SDS and LDL-C levels (P = 0.006). Furthermore, a nonlinear relationship was observed between IGF-1 SDS and LDL-C by smooth curve fitting after adjusting for possible confounders. A multivariate piecewise linear regression model revealed a significant negative correlation between IGF-1 SDS and LDL-C when the IGF-1 level was greater than − 2 SDS (β − 0.07, 95% CI -0.12, − 0.02; P = 0.006). However, we did not observe a significant relationship between IGF-1 SDS and LDL-C when the IGF-1 level was lower than − 2 SDS (β 0.08, 95% CI -0.02, 0.17; P = 0.119).
Conclusion
This study demonstrated a nonlinear relationship between IGF-1 and LDL-C independent of other potential confounding factors, suggesting that circulating IGF-1 may contribute to the regulation of LDL-C levels, thus meriting further investigation.
Electronic supplementary material
The online version of this article (10.1186/s12944-019-1062-z) contains supplementary material, which is available to authorized users.
Keywords: Insulin-like growth factor-1, Low-density lipoprotein cholesterol, Coronary heart disease, Short stature
Introduction
Dyslipidaemia in childhood and adolescence is a strong marker of atherogenic risk and may contribute to the development of coronary heart disease (CHD) in adulthood [1, 2]. Childhood atherosclerosis may not be obvious, but atherosclerosis is a multifactorial disease with its roots in childhood [3, 4]. Endocrinologists have paid considerable attention to screening lipid profiles and have demonstrated the related factors in youths, especially in obese children, who are prone to having unfavourable lipid profiles [5–7]. Some studies have provided evidence that short stature is a risk factor associated with CHD [8, 9] and non-high-density lipoprotein (non-HDL) cholesterol levels even after adjustment for body mass index (BMI) in adults [10], and recent studies have reported that an inverse relationship between height and non-HDL cholesterol is also exists in children [11–13]. Therefore, these studies suggest the need to analyse the lipid profiles and the associated factors, especially in children and adolescents with short stature.
The accumulation of low-density lipoprotein cholesterol (LDL-C), which forms fatty streaks in the intima is the earliest morphological change occurring in the development of atherosclerosis [14, 15]. Previous longitudinal prospective cohort studies have demonstrated that compared to the other lipid indexes, LDL-C evaluated in childhood is the strongest predictor for increased risk of adulthood dyslipidaemia and CHD [16, 17]. The assessment of childhood CHD risk based on LDL-C may be useful for preventing cardiovascular disease in adulthood [16]. Furthermore, a recent study reported that a normal LDL-C levels were associated with subclinical atherosclerosis in a middle-aged population of 4184 adults aged between 40 and 54 years of age [18]. The LDL-C level is typically multifactorial, and it is well-established that BMI and LDL-C have a positive relationship in obese children [19–21] and non-obese children [22–24]. Children and adolescents with short stature have increased LDL-C levels [11], but this relationship has not received much attention. A prospective cohort study with 20 years of follow-up has reported that the childhood LDL-C levels are useful for predicting adult dyslipidaemia and other cardiovascular risks [25]. Furthermore, Nelson CP et al. performed a pathway analysis of height-associated factors and identified a primary association between a genetically determined short stature and an increased risk of CHD, which is partially explained by the association between short stature and an adverse lipid profile [26].
As a growth factor, insulin-like growth factor-1 (IGF-1) also plays an important role in the lipid metabolism [27]. According to De Ita JR et al., IGF-1-deficient mice exhibit dysregulated expression of lipid metabolism-related genes and genes encoding enzymes involved in cholesterol synthesis in the liver [28]. A cross-sectional observation in the large-scale community based Framingham Heart Study demonstrated that lower IGF-1 levels are associated with metabolic syndrome [29]. Additionally, an intervention study have demonstrated that short term treatment with recombinant human growth hormone (rhGH) improved lipid metabolism [30]. Moreover, the IGF-1 level in the short-stature population is usually below the normal reference range [31].
Previous studies have shown a controversial relationship between IGF-1 and LDL-C [32] and there was a lack of a database of children and adolescents with short stature. Here, we screened the lipid profiles of children and adolescents with short stature at a single centre in China and investigated the relationship between IGF-1 and LDL-C levels in this population.
Methods
Subjects
The study group consisted of 557 children and adolescents with short stature (409 males and 148 females, from 3 to 18 years of age) who were enrolled from May 2013 to December 2017. A retrospective cross-sectional study was carried out at the Department of Endocrinology, Affiliated Hospital of Jining Medical University, Jining, Shandong, China. The subjects were selected based on the following inclusion criteria: short stature, which is defined as a condition in which the individual’s height is more than two standard deviations (SD) below the population mean for the relevant age and gender [35]; normal weight and height at birth; and the absence of chronic disease. The exclusion criteria included children with chromosomal abnormalities, skeletal dysplasia, genetic metabolic diseases, thyroid dysfunction, or abnormal liver function.
Anthropometric measurement
Anthropometric measurements included measurements of height, weight, systolic blood pressure (SBP), diastolic blood pressure (DBP), and pubertal stage. Height and weight were assessed according to standard procedures with the participants in light clothing, with no shoes. Body height was measured to the nearest 0.1 cm by using a Best Industrial Stadiometer (Nantong Best Industrial Co, Ltd., Jiangsu; China). A scale with a capacity of 120 kg (Wuxi Weigher Factory Co, Ltd., Jiangsu; China) was used to measure body weight to the nearest 0.1 kg. Height and weight were expressed as the standard deviation scores (SDS) based on normative values for Chinese children [36]. BMI was calculated as weight divided by height in metres squared, and the BMI SDS was calculated according to 2009 growth charts for Chinese children and adolescents [37]. Pubertal stage was evaluated by physical examination according to the Tanner stages [38]. The criteria for prepuberty were as follows: for boys, testicular volume less than 4 mL with no pubic hair; for girls, no breast development or pubic hair.
Laboratory measurements
Fasting blood samples were obtained from all participants to measure the serum IGF-1 level and other laboratory parameters.
Serum IGF-1 concentrations were estimated based on a chemiluminescence assay (DPC IMMULITE 1000 analyser, SIEMENS, Germany) with an intra-assay and inter-assay coefficient of variation of 3.0 and 6.2%, respectively; the IGF-1 SDS, adjusted for age and sex, was calculated according to IGF-1 levels of healthy Japanese children and adolescents of the same age and sex [39]. Total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), LDL-C, very low density lipoprotein cholesterol (VLDL-C), triglyceride (TG), alanine aminotransferase (ALT), fasting plasma glucose (FPG) and uric acid (UA) levels were determined using an auto biochemical analyser (Cobas c702, Roche; Shanghai, China). LDL-C dyslipidaemia was defined according to an expert consensus regarding Chinese child and adolescent dyslipidaemia [40].
Statistical analysis
The continuous variables are expressed as the means ± standard deviations, and the categorical data are expressed as a number (percentage). A univariate analysis model was used to determine the significance of the association between LDL-C and IGF-1 SDS as well as the other independent variables. We then investigated the relationship between LDL-C and IGF-1 SDS using smooth curve fitting after adjusting for potential confounders. Finally, we further applied a multivariate piecewise linear regression model to examine the threshold association of LDL-C and IGF-1 SDS. Statistical significance was indicated by as a two-sided P value < 0.05. All analyses were performed using EmpowerStats (http://www.empowerstats.com, X&Y Solutions, Inc., Boston, MA) and R 3.4.3 (http://www.r-project.org).
Results
Clinical and laboratory characteristics of the subjects
The clinical characteristics of all participants were described in Table 1. A total of 557 children and adolescents with short stature and a mean age of 10.1 ± 3.5 years old were included in the study. The mean height SDS of the participants was − 2.72 ± 0.62. Of the subjects, 409 (73.43%) children were male. The majority of the children, 421 (75.58%) were prepubescent. The mean IGF-1 SDS and LDL-C level were − 1.07 ± 1.31 and 2.06 ± 0.54 mmol/L, respectively. The rate of LDL-C dyslipidaemia in the children and adolescents with short stature was 1.97%, and the rate of occurrence of critical LDL-C dyslipidaemia was 4.13%.
Table 1.
Clinical and laboratory characteristics of the subjects
| Characteristics | All |
|---|---|
| N | 557 |
| Age (years) | 10.1 ± 3.5 |
| Male/Female (N) | 409/148 |
| Prepuberty/Puberty (N) | 421/136 |
| Height (cm) | 124.81 ± 17.67 |
| Height SDS | −2.72 ± 0.62 |
| Weight (kg) | 27.18 ± 10.36 |
| BMI (kg/m2) | 16.80 ± 2.78 |
| BMI SDS | −0.28 ± 1.17 |
| IGF-1 (ug/L) | 179.80 ± 121.64 |
| IGF-1 SDS | −1.07 ± 1.31 |
| SBP (mmHg) | 104.12 ± 11.63 |
| DBP (mmHg) | 62.81 ± 8.86 |
| FPG (mmol/L) | 4.80 ± 0.68 |
| ALT (U/L) | 16.32 ± 9.05 |
| UA (umol/L) | 265.76 ± 71.18 |
| TG (mmol/L) | 0.71 ± 0.33 |
| TC (mmol/L) | 3.84 ± 0.69 |
| HDL-C (mmol/L) | 1.36 ± 0.30 |
| VLDL-C (mmol/L) | 0.43 ± 0.26 |
| LDL-C (mmol/L) | 2.06 ± 0.54 |
| Critical value of LDL-C, N (%) | 23 (4.13%) |
| High value of LDL-C, N (%) | 11 (1.97%) |
Abbreviations: Height SDS: height standard deviation scores; BMISDS: body mass index standard deviation scores; IGF-1 SDS: insulin like growth factor-1 standard deviation scores; SBP: systolic blood pressure, DBP: diastolic blood pressure; FPG: fasting plasma glucose; ALT: alanine aminotransferase; UA: uric acid; TG: triglyceride; TC: total cholesterol; HDL-C: high density lipoprotein-cholesterol; LDL-C: low density lipoprotein cholesterol; VLDL-C: very low density lipoprotein-cholesterol. According to children and adolescents dyslipidemia consensus. Critical value of LDL-C was 2.85–3.34 mmol/L; High value was defined as of LDL-C level ≥ 3.37 mmol/L. Continuous variables are expressed as means ± standard deviations and categorical data using number (percentage)
Factors correlated with LDL-C in the subjects
Univariate linear regression analysis was performed to determine the relationships between clinical parameters and LDL-C. As shown in Table 2, for the unadjusted model, we observed a significant negative correlation between IGF-1 SDS and LDL-C (P = 0.006). Other variables that remained significantly associated with LDL-C were age, weight, BMI SDS, TG, TC, HDL-C, VLDL-C and pubertal stage (P < 0.05). No significant association was observed between LDL-C and sex, height SDS, SBP, DBP, FPG, ALT or UA (P > 0.05).
Table 2.
Factors correlated to LDL-C by a univariate analysis
| β | (95% CI) | P value | |
|---|---|---|---|
| Age (years) | −0.03 | (− 0.04, − 0.02) | <0.001 |
| Height SDS | 0.04 | (− 0.03, 0.11) | 0.265 |
| Weight (kg) | 0.00 | (−0.01, − 0.00) | 0.034 |
| BMI SDS | 0.07 | (0.04, 0.11) | <0.001 |
| IGF1 SDS | −0.05 | (− 0.08, − 0.01) | 0.006 |
| SBP (mmHg) | − 0.00 | (− 0.01, 0.00) | 0.507 |
| DBP (mmHg) | −0.00 | (− 0.01, 0.00) | 0.712 |
| FPG (mmol/L) | −0.06 | (− 0.13, 0.01) | 0.115 |
| ALT (U/L) | 0.00 | (−0.00, 0.01) | 0.119 |
| UA (umol/L) | 0.00 | (−0.00, 0.00) | 0.456 |
| TG (mmol/L) | 0.18 | (0.05, 0.32) | 0.008 |
| TC (mmol/L) | 0.64 | (0.61, 0.68) | <0.001 |
| HDL-C (mmol/L) | 0.15 | (0.00, 0.30) | 0.046 |
| VLDL-C (mmol/L) | 0.20 | (0.02, 0.37) | 0.027 |
| Sex | |||
| Male | 0 | ||
| Female | 0.06 | (−0.04, 0.16) | 0.222 |
| Pubertal stage | |||
| In prepuberty | 0 | ||
| In puberty | −0.14 | (−0.25, − 0.04) | 0.007 |
Abbreviations: Height SDS: height standard deviation scores; BMISDS: body mass index standard deviation scores; IGF-1 SDS: insulin like growth factor-1 standard deviation scores; SBP: systolic blood pressure, DBP: diastolic blood pressure; FPG: fasting plasma glucose; ALT: alanine aminotransferase; UA: uric acid; TG: triglyceride; TC: total cholesterol; HDL-C: high density lipoprotein-cholesterol; LDL-C: low density lipoprotein cholesterol; VLDL-C: very low density lipoprotein-cholesterol. P < 0.05 is considered to be statistically significant
Independent correlation between IGF-1 SDS and LDL-C by multivariate piecewise linear regression
As shown in Fig. 1, smooth curve fitting was performed after adjusting for possible confounding factors, including weight, BMI SDS, TG, HDL-C, VLDL-C and pubertal stage. The participants’ LDL-C levels exhibited an nonlinear relationships with IGF-1 SDS and the resulting curve exhibited a two-stage change and a breakpoint. When the IGF-1 SDS level was greater than the breakpoint, there was an inverse relationship between IGF-1 SDS and LDL-C; however, if the value was less than the breakpoint, there was a positive relationship between IGF-1 SDS and LDL-C. As shown in Table 3, we further analysed the threshold effect based on curve fitting, and the data indicated that the inflection point of the IGF-1 SDS was − 2. Specifically, LDL-C levels decreased as the IGF-1 SDS increased when the IGF-1 SDS was greater than − 2 (β − 0.07, 95% CI -0.12, − 0.02; P = 0.006). However, the LDL-C levels displayed an increasing trend as the IGF-1 SDS increased when the IGF-1 SDS was less than − 2, but the difference was not statistically significant (β 0.08, 95% CI -0.02, 0.17; P = 0.119). In addition, as shown in Additional file 1: Table S1, after stratification by pubertal stage, a nonlinear relationship between IGF-1 SDS and LDL-C remained.
Fig. 1.
The relationship between IGF-1 SDS and LDL-C by smooth curve fitting. Adjustment variables: weight, BMI SDS, TG, HDL-C, VLDL-C, pubertal stage
Table 3.
The independent correlation between IGF-1 SDS on LDL-C by multivariate piecewise linear regression
| Inflection point of IGF-1 SDS | β (95% CI) | P value |
|---|---|---|
| < −2.0 | 0.08 (− 0.02, 0.17) | 0.119 |
| > −2.0 | − 0.07 (− 0.12, − 0.02) | 0.006 |
Abbreviations: IGF-1 SDS: insulin likes growth factor-1 standard deviation scores; LDL-C: low density lipoprotein-cholesterol. Adjusted for weight, BMI SDS, TG, HDL-C, VLDL-C, pubertal stage. P < 0.05 is considered to be statistically significant
Discussion
In this study, we observed that there was a nonlinear relationship between IGF-1 SDS and LDL-C in children and adolescents with short stature, and the IGF-1 SDS turning point was − 2. The negative relationship between IGF-1 SDS and LDL-C was significant only when the IGF-1 SDS reached the inflection point.
In the children and adolescents with short stature, we observed that the IGF-1 SDS was a protective factor against elevated LDL-C levels. This result was consistent with the findings of previous clinical studies, a study conducted in women with primary hypothyroidism showed that IGF-1 was a determinant of the concentration of LDL-C and that there was a negative relationship between IGF-1 and LDL-C in individuals with hypothyroidism [32]. In addition, there is a similar relationship between IGF-1 and LDL-C in mildly hypercholesterolemic women [33]. However, this relationship was not significant in a study carried out in healthy subjects [34], and the differences in the clinical characteristics and recruitment criteria among these studies may explain this divergent result. Furthermore, we revealed a nonlinear relationship between IGF-1 SDS and LDL-C, suggesting that a certain IGF-1 level should be maintained to modulate the serum LDL-C concentration. The potential mechanisms of this phenomenon can be explained as follows: IGF-1 can downregulate 12/15-lipoxygenase, thereby suppressing lipid oxidation [41], and decreased IGF-1 may impair the expression of genes involved in lipid catabolism and dysregulate the expression of related proteins encoding low-density lipoprotein receptors [28]. Furthermore, it was found that the level of LDL-C is decreased in children with growth hormone deficiency (GHD) as a result of rhGH replacement therapy. These findings support the notion that IGF-1 plays a relevant role in modulating the serum LDL-C concentration.
Obese children are prone to dyslipidaemia, and our study also showed that the BMI SDS had a positive relationship with LDL-C. This finding was consistent with the results of a previous study reporting a positive relationship between LDL-C and BMI in a large population-based sample of Japanese school children [42]. Furthermore, serum lipid levels and BMI in childhood correlate strongly with these values in middle age [43]. The correlation between short stature and elevated plasma LDL-C levels has also been observed in school children in fifth grade [11], however, we did not find an association between LDL-C and height, which may be because the study subjects with a height SDS of no more than − 2 were limited.
In addition, this study also revealed a negative relationship between age, pubertal stage and LDL-C. Namely, the data show small decreases in LDL-C as age increases, which is consistent with a decreases in LDL-C levels occurring along with advancing sexual development. This finding was consistent with the recent study by Eissa that showed that non-HDL levels decrease with progressing Tanner stage [44].
The relationship between IGF-1 SDS and LDL-C has been considered in only a few papers. In our report, we confirm that this relationship can be observed in children and adolescents with short stature. The level of IGF-1 level is related not only to growth but also to lipid metabolism.
The present study has several limitations. The first limitation is that the cross-sectional design of this study does not allow us to determine causality. Second, the negative relationship between LDL-C and IGF-1 SDS needs further validation in a prospective study. Third, further investigation is necessary to follow up on age changes to determine whether the relationship between IGF-1 and LDL-C would remain as individuals age. Additionally, additional research is needed to fully understand the mechanism underlying the association between IGF-1 and LDL-C.
Conclusion
In conclusion, this study described a nonlinear relationship between IGF-1 and LDL-C levels in children and adolescents with short stature after adjusting for potential confounders. This finding suggests that in children and adolescents with short stature, the level of IGF-1 is not related only to its growth effect, but also to its lipid metabolism effects.
Additional file
Table S1. The independent correlation between IGF-1 SDS and LDL-C levels identified using multivariate piecewise linear regression analysis in groups stratified according to before and after puberty. (DOC 28 kb)
Acknowledgements
We thank all the partners and staff who helped us in the process of this study.
Funding
This study was supported by the Jining Science and Technology Bureau (No. 2017SMNS007).
Availability of data and materials
The datasets used and/or analysed in the current study are available from the corresponding authors upon reasonable request.
Abbreviations
- ALT
alanine aminotransferase
- BMISDS
body mass index standard deviation scores
- CHD
coronary heart disease
- DBP
diastolic blood pressure
- FPG
fasting plasma glucose
- HDL-C
high density lipoprotein-cholesterol
- Height SDS
height standard deviation scores
- IGF-1 SDS
insulin like growth factor-1 standard deviation scores
- LDL-C
low density lipoprotein cholesterol
- non-HDL
non-high density lipoprotein.
- SBP
systolic blood pressure
- TC
total cholesterol
- TG
triglyceride
- UA
uric acid
- VLDL-C
very low density lipoprotein-cholesterol
Authors’ contributions
QZ carried out the studies and drafted the manuscript. YJ and YC helped with the statistical analysis. MZ and HP participated in the design of the study and revised the manuscript. BJ participated in data collection. BB participated in the conceptualization and design of the study, revised the manuscrip critically for important intellectual content and final provided approval of the version to be published. All authors read and approved the final manuscript.
Ethics approval and consent to participate
The study was approved by the Human Ethics Committee of the Affiliated Hospital of Jining Medical University (History: 01-07-2015, approval number: 019). All of the families of the patients were informed of the aims of the study, and written informed consent forms were signed by all of the participants’ parents.
Consent for publication
All authors have read and approved the content, and they agree to submit it for consideration for publication in the journal.
Competing interests
The authors declare that they have no competing interests.
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Contributor Information
Qianqian Zhao, Email: zhaoqian930915@163.com.
Yingzhe Jiang, Email: jiangyingzhe@me.com.
Mei Zhang, Email: zhangmeijn@163.com.
Yuntian Chu, Email: jimchuy@yahoo.com.
Baolan Ji, Email: jibaolan@yeah.net.
Hui Pan, Email: panhui20111111@163.com.
Bo Ban, Email: banbo2011@163.com.
References
- 1.Lozano Paula, Henrikson Nora B., Dunn John, Morrison Caitlin C., Nguyen Matt, Blasi Paula R., Anderson Melissa L., Whitlock Evelyn P. Lipid Screening in Childhood and Adolescence for Detection of Familial Hypercholesterolemia. JAMA. 2016;316(6):645. doi: 10.1001/jama.2016.6176. [DOI] [PubMed] [Google Scholar]
- 2.Haney EM, Huffman LH, Bougatsos C, Freeman M, Steiner RD, Nelson HD. Screening and treatment for lipid disorders in children and adolescents: systematic evidence review for the US preventive services task force. Pediatrics. 2007;120:189–214. doi: 10.1542/peds.2006-1801. [DOI] [PubMed] [Google Scholar]
- 3.Rd NW, Freedman DS, Voors AW, Gard PD, Srinivasan SR, Cresanta JL, Williamson GD, Webber LS, Berenson GS. Relation of serum lipoprotein levels and systolic blood pressure to early atherosclerosis. The Bogalusa Heart Study New Engl J Med. 1986;314:138–144. doi: 10.1056/NEJM198601163140302. [DOI] [PubMed] [Google Scholar]
- 4.Raitakari OT, Juonala M, Kähönen M, Taittonen L, Laitinen T, Mäkitorkko N, Järvisalo MJ, Uhari M, Jokinen E, Rönnemaa T. Cardiovascular risk factors in childhood and carotid artery intima-media thickness in adulthood: the cardiovascular risk in young Finns study. JAMA. 2004;13:2277–2283. doi: 10.1001/jama.290.17.2277. [DOI] [PubMed] [Google Scholar]
- 5.Cai L, Wu Y, Cheskin LJ, Wilson RF, Wang Y. Effect of childhood obesity prevention programmes on blood lipids: a systematic review and meta-analysis. Obes Rev. 2014;15:933–944. doi: 10.1111/obr.12227. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 6.Selin E, Derya T, Ahmet US, Fatma KK, Fatma D. Prevalence of dyslipidemia and associated factors in obese children and adolescents. J Clin Res Pediatr E. 2015;7:228–234. doi: 10.4274/jcrpe.1867. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7.Kit BK, Kuklina E, Carroll MD, Ostchega Y, Freedman DS, Ogden CL. Prevalence of and trends in dyslipidemia and blood pressure among US children and adolescents, 1999-2012. JAMA Pediatr. 2015;169:272–279. doi: 10.1001/jamapediatrics.2014.3216. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 8.Paajanen TA, Oksala NKJ, Kuukasjärvi P, Karhunen PJ. Short stature is associated with coronary heart disease: a systematic review of the literature and a meta-analysis. Eur Heart J. 2010;31:1802–1809. doi: 10.1093/eurheartj/ehq155. [DOI] [PubMed] [Google Scholar]
- 9.Gertler MM, Garn SM, White PD. Young candidates for coronary heart disease. J Am Med Assoc. 1951;147:621–625. doi: 10.1001/jama.1951.03670240005002. [DOI] [PubMed] [Google Scholar]
- 10.Henriksson KM, Lindblad U, Ågren B, Nilsson-Ehle P, Råstam L. Associations between body height, body composition and cholesterol levels in middle-aged men. The coronary risk factor study in southern Sweden (CRISS) Eur J Epidemiol. 2001;17:521–526. doi: 10.1023/A:1014508422504. [DOI] [PubMed] [Google Scholar]
- 11.Pyles LA, Lilly C, Mullett C, Polak E, Elliott E, Neal WA. LDL cholesterol level in fifth grade school children associates with stature. J Lipid Res. 2017;58:78816–78820. doi: 10.1194/jlr.P078816. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 12.Ferrie JE, Langenberg C, Shipley MJ, Marmot MG. Birth weight, components of height and coronary heart disease: evidence from the Whitehall II study. Int J Epidemiol. 2006;35:1532–1542. doi: 10.1093/ije/dyl184. [DOI] [PubMed] [Google Scholar]
- 13.Jolliffe CJ, Janssen I. Distribution of lipoproteins by age and gender in adolescents. Circulation. 2006;114(10):1056–1062. doi: 10.1161/CIRCULATIONAHA.106.620864. [DOI] [PubMed] [Google Scholar]
- 14.Skålén K, Gustafsson M, Rydberg EK, Hultén LM, Wiklund O, Innerarity TL, Borén J. Subendothelial retention of atherogenic lipoproteins in early atherosclerosis. Nature. 2002;417:750–754. doi: 10.1038/nature00804. [DOI] [PubMed] [Google Scholar]
- 15.Joris I, Zand T, Nunnari JJ, Krolikowski FJ, Majno G. Studies on the pathogenesis of atherosclerosis. I. Adhesion and emigration of mononuclear cells in the aorta of hypercholesterolemic rats. Am J Pathol. 1983;113:341–358. [PMC free article] [PubMed] [Google Scholar]
- 16.Bao W, Srinivasan SR, Wattigney WA, Bao W, Berenson GS. Usefulness of childhood low-density lipoprotein cholesterol level in predicting adult dyslipidemia and other cardiovascular risks. The Bogalusa heart study. Arch Intern Med. 1996;156:1315–1320. doi: 10.1001/archinte.1996.00440110083011. [DOI] [PubMed] [Google Scholar]
- 17.Srinivasan SR, Frontini MG, Xu J, Berensonet GS. Utility of childhood non-high-density lipoprotein cholesterol levels in predicting adult dyslipidemia and other cardiovascular risks. The Bogalusa heart study. Pediatrics. 2006;118:201–206. doi: 10.1542/peds.2005-1856. [DOI] [PubMed] [Google Scholar]
- 18.Fernandez-Friera L, Fuster V, Lopez-Melgar B, Oliva B, Garcia-Ruiz JM, Mendiguren J, Bueno H, Pocock S, Ibanez B, Fernandez-Ortiz A, Sanz J. Normal LDL-cholesterol levels are associated with subclinical atherosclerosis in the absence of risk factors. J Am Coll Cardiol. 2017;70:2979–2991. doi: 10.1016/j.jacc.2017.10.024. [DOI] [PubMed] [Google Scholar]
- 19.Kaur S, Kapil U. Dyslipidemia amongst obese children in national capital territory (NCT) of Delhi. Indian J Pediatr. 2011;78:55–57. doi: 10.1007/s12098-010-0263-2. [DOI] [PubMed] [Google Scholar]
- 20.Mahin H, Mahnaz S, Mohammad MA, Mojgan S. Anthropometric indices associated with dyslipidemia in obese children and adolescents: a retrospective study in Isfahan. Arya Atheroscler. 2011;7:31–39. [PMC free article] [PubMed] [Google Scholar]
- 21.Bijari B, Taheri F, Chahkandi T, Kazemi T, Namakin K, Zardast M. The relationship between serum lipids and obesity among elementary school in Birjand: a case control study. J Res Health Sci. 2015;15:83–87. [PubMed] [Google Scholar]
- 22.Rahman AAA. Lipid profile in relation to anthropometric measurements among college male students in Riyadh, Saudi Arabia: a cross-sectional study. Int J Biomed Sci. 2011;7:112–119. [PMC free article] [PubMed] [Google Scholar]
- 23.Azita F, Asghar Z, Gholam-Reza S. Relationship of body mass index with serum lipids in elementary school students. Indian J Pediatr. 2009;76(7):729–731. doi: 10.1007/s12098-009-0169-z. [DOI] [PubMed] [Google Scholar]
- 24.Shirasawa T, Ochiai H, Ohtsu T, Nishimura R, Morimoto A, Hoshino H, Tajima N, Kokaze A. LDL-cholesterol and body mass index among Japanese schoolchildren: a population-based cross-sectional study. Lipids Health Dis. 2013;12:77. doi: 10.1186/1476-511X-12-77. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 25.Magnussen CG, Raitakari OT, Thomson R, Juonala M, Patel DA, Viikari JS, Marniemi J, Srinivasan SR, Berenson GS. Utility of currently recommended pediatric dyslipidemia classifications in predicting dyslipidemia in adulthood: evidence from the childhood determinants of adult health (CDAH) study, cardiovascular risk in young Finns study, and Bogalusa heart study. Circulation. 2008;117:32–42. doi: 10.1161/CIRCULATIONAHA.107.718981. [DOI] [PubMed] [Google Scholar]
- 26.Nelson CP, Hamby SE, Saleheen D, et al. Genetically determined height and coronary artery disease. N Engl J Med. 2015;372:1608–1618. doi: 10.1056/NEJMoa1404881. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 27.Aguirre GA, Rodríguez DIJ, De LGRG. Castilla-Cortazar I. insulin-like growth factor-1 deficiency and metabolic syndrome. J Transl Med. 2016;14:1–23. doi: 10.1186/s12967-015-0762-z. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 28.De Ita JR, Castilla-Cortázar I, Aguirre GA, Sánchez-Yago C, Santos-Ruiz MO, Guerra-Menéndez L, Martín-Estal I, García-Magariño M, Lara-Díaz VJ, Puche JE, Muñoz U. Altered liver expression of genes involved in lipid and glucose metabolism in mice with partial IGF-1 deficiency: an experimental approach to metabolic syndrome. J Transl Med. 2015;13, 13(1). [DOI] [PMC free article] [PubMed]
- 29.Lam CS, Chen MH, Lacey SM, Yang Q, Sullivan LM, Xanthakis V, Safa R, Smith HM, Peng X, Sawyer DB, Vasan RS. Circulating insulin-like growth factor-1 and its binding protein-3: metabolic and genetic correlates in the community. Arterioscler Thromb Vasc Biol. 2010;30:1479–1484. doi: 10.1161/ATVBAHA.110.203943. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 30.Kubo T, Furujo M, Takahashi K, Hyodo Y, Tsuchiya H, Hattori M, Fujinaga S, Urayama K. Effects of growth hormone treatment on lipid profiles. Indian J Pediatr. 2017:1–5. [DOI] [PubMed]
- 31.Zelazowska-Rutkowska B, Trusiak M, Bossowski A, Cylwik B. Diagnostic usefulness of insulin-like growth factor 1 and insulin-like growth factor binding protein 3 in children with suspected pituitary dwarfism. Clin Lab. 2018;64:759–765. doi: 10.7754/Clin.Lab.2018.171126. [DOI] [PubMed] [Google Scholar]
- 32.Hoogerbrugge-Vd LN, Jansen H, Hülsmann WC, Birkenhäger JC. Relationship between insulin-like growth factor-I and low-density lipoprotein cholesterol levels in primary hypothyroidism in women. J Endocrinol. 1989;123:341–345. doi: 10.1677/joe.0.1230341. [DOI] [PubMed] [Google Scholar]
- 33.Prewitt TE, Unterman TG, Glick R, Cole TG, Schmeisser D, Bowen PE, Langenberg P. Insulin-like growth factor I and low-density-lipoprotein cholesterol in women during high- and low-fat feeding. Am J Clin Nutr. 1992;55:381–384. doi: 10.1093/ajcn/55.2.381. [DOI] [PubMed] [Google Scholar]
- 34.Hopkins KD, Lehmann ED, Parker JR, Gosling RG. Insulin-like growth factor binding protein-1 is correlated with low density lipoprotein cholesterol in normal subjects. J Endocrinol. 1994;140(3):521–524. doi: 10.1677/joe.0.1400521. [DOI] [PubMed] [Google Scholar]
- 35.Rogol AD, Hayden GF. Etiologies and early diagnosis of short stature and growth failure in children and adolescents. J Pediatr. 2014;164:S1–14. doi: 10.1016/j.jpeds.2014.02.027. [DOI] [PubMed] [Google Scholar]
- 36.Li H, Ji CY, Zong XN, Zhang YQ. Height and weight standardized growth charts for Chinese children and adolescents aged 0 to 18 years. Chinese J Pediatr. 2009;47:487–492. [PubMed] [Google Scholar]
- 37.Li H, Ji CY, Zong XN, Zhang YQ. Body mass index growth curves for Chinese children and adolescents aged 0 to 18 years. Chinese J Pediatr. 2009;47:493–498. [PubMed] [Google Scholar]
- 38.Wright CM, Ahmed L, Dunger DB, Preece MA, Cole TJ, Butler G. Can we characterise growth in puberty more accurately?Validation of a new puberty phase specific(PPS)growth chart. Arch Dis Child. 2012;97(S1):A100. [Google Scholar]
- 39.Isojima T, Shimatsu A, Yokoya S, Chihara K, Tanaka T, Hizuka N, Teramoto A, Tatsumi KI, Tachibana K, Katsumata N. Standardized centile curves and reference intervals of serum insulin-like growth factor-I (IGF-I) levels in a normal Japanese population using the LMS method. Endocr J. 2012;59:771–780. doi: 10.1507/endocrj.EJ12-0110. [DOI] [PubMed] [Google Scholar]
- 40.Subspecialty Group of Child Health Care The Society of Pediatrics, Chinese Medical Association. Experts consensus for prevention and treatment of dyslipidemia in children and adolescents. Chinese J Pediatr. 2009;47:426–428. [PubMed] [Google Scholar]
- 41.Sukhanov S, Snarski P, Vaughn C, Lobellerich P, Kim C, Higashi Y, Shai SY, Delafontaine P. Insulin-like growth factor I reduces lipid oxidation and foam cell formation via downregulation of 12/15-lipoxygenase. Atherosclerosis. 2015;238:313–320. doi: 10.1016/j.atherosclerosis.2014.12.024. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 42.Shirasawa T, Ochiai H, Ohtsu T, Nishimura R, Morimoto A, Hoshino H, Tajima N, Kokaze A. LDL-cholesterol and body mass index among Japanese school children: a population-based cross-sectional study. Lipids Health Dis. 2013;12:77. doi: 10.1186/1476-511X-12-77. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 43.Juhola J, Magnussen CG, Viikari JS, Kähönen M, Hutri-Kähönen N, Jula A, Lehtimäki T, Åkerblom HK, Pietikäinen M, Laitinen T, Jokinen E, Taittonen L, Raitakari OT, Juonala M. Tracking of serum lipid levels, blood pressure, and body mass index from childhood to adulthood: the cardiovascular risk in young Finns study. J Pediatr. 2011;159:584–590. doi: 10.1016/j.jpeds.2011.03.021. [DOI] [PubMed] [Google Scholar]
- 44.Eissa MA, Mihalopoulos NL, Holubkov R, Dai S, Labarthe DR. Changes in fasting lipids during puberty. J Pediatr. 2016;170:199–205. doi: 10.1016/j.jpeds.2015.11.018. [DOI] [PMC free article] [PubMed] [Google Scholar]
Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.
Supplementary Materials
Table S1. The independent correlation between IGF-1 SDS and LDL-C levels identified using multivariate piecewise linear regression analysis in groups stratified according to before and after puberty. (DOC 28 kb)
Data Availability Statement
The datasets used and/or analysed in the current study are available from the corresponding authors upon reasonable request.