FIG. 2.
Disruption of the interaction between NcoR1 and TRα increases bone mass, mineralization, and strength in WT mice. (A) X-ray microradiography images of femurs from male WT and NCoR1ΔID/ΔID mice at 14 weeks of age. Gray-scale images and pseudocolored images in which low BMC is indicated in green and high BMC in pink. Cumulative frequency histogram of relative BMC (n = 6–8 per group). ***p < 0.001 vs. WT; Kolmogorov–Smirnov test. Graphs show lengths of femurs, humeri, and vertebrae. Data are shown as the mean ± SEM (n = 6–8 per group); Student's t-test. (B) Micro-CT images of mid-diaphysis cortical bone. Graphs showing Ct.Th, cortical BMD, and Ct.Po. Data are shown as the mean ± SEM (n = 6–8 per group apart from Ct.Po, n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001; Student's t-test. (C) Micro-CT images of distal femur trabecular bone. Graphs showing BV/TV, Tb.N, Tb.Th, and Tb.Sp. Data are shown as the mean ± SEM (n = 6–8 per group). (D) Representative load displacement curves for humerus three-point bend testing. Yield load, maximum load, fracture load, and stiffness. Data are the mean ± SEM (n = 6–8 per group). *p < 0.05, ***p < 0.001, and ****p < 0.0001 vs. WT; Student's t-test. Scale bars in (A), (B) and (C) = 1 mm. Data from the same group of untreated WT mice are included in Figures 1–4 to facilitate comparison across groups. Color images are available online.