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. Author manuscript; available in PMC: 2019 May 28.
Published in final edited form as: Shock. 2019 May;51(5):569–579. doi: 10.1097/SHK.0000000000001300

Table 1.

Primer sequences used for real-time PCR

Target Primer sequences (5’-3’) Product size
(bp)
IL-1β Forward 5’-GCC CAT CCT CTG TGA CTC AT-3’
Reverse 5’- AGG CCA CAG GTA TTT TGT CG-3’
230
IL-6 Forward 5’-AGCCAGAGTCCTTCAGAGA-3’
Reverse 5’ -TCCTTAGCCACTCCTTCTGT-3’
146
IL-10 Forward 5'-GGT TGC CAA GCC TTA TCG GA-3’
Reverse 5'-ACC TGC TCC ACT GCC TTG CT-3’
191
TNF-α Forward 5’ –TGG GAC AGT GAC CTG GAC TGT-3’
Reverse 5’ - TTC GGA AAG CCC ATT TGA GT-3’
67
CXCL2 Forward 5'-CAG AAGTCA TAG CCA CTCTCAAG-3'
Reverse 5'-CTT TCC AGGTCA GTT AGC CTT-3’
119
MCP-1 Forward 5'-GCTCAGCCAGATGCAGTTAA-3'
Reverse 5'-TCTTGAGCTTGGTGACAAAAACT-3'
129