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Inhibitors of lipoprotein biogenesis Glb and Cpd2 activate Cpx through NlpE. Cells were treated with either Glb or Cpd2 lipoprotein trafficking inhibitors (or DMSO vehicle control) for 20 min. RNA was then extracted and subjected to qRT-PCR to quantitate levels of cpxP mRNA. Ksg-treated cells (+Ksg) were treated with a sub-MIC of Ksg for 15 min prior to Glb or Cpd2 treatment (see Materials and Methods). Data are means ± standard errors of the means.
