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. 2019 May 1;24(9):1700. doi: 10.3390/molecules24091700

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Blockade of TW-37-induced apoptosis by ectopic expression of HO-1. YD-15 cells were transiently transfected with pcDNA3.1 or pcDNA3.1-HO-1 for 6 h, and then treated with DMSO or TW-37 for 48 h. (A) Protein levels of HO-1 and cleaved PARP were analyzed by western blotting. Actin was used as a loading control. Bar graphs are expressed as mean ± SD from three independent experiments. *, p < 0.05. (B) FACS analysis of annexin V/PI staining. The annexin V-positive cells are expressed as the percentage of apoptotic cells. The results are expressed as mean ± SD from three independent experiments. *, p < 0.05. (C) FACS analysis of PI staining. The histogram represents the distribution of the cell cycle and the percentage of cells in the sub-G1 phase are expressed as mean ± SD from three independent experiments. *, p < 0.05.