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Oncology Letters logoLink to Oncology Letters
. 2019 May 6;18(1):321–329. doi: 10.3892/ol.2019.10317

Annexin A1-mediated inhibition of inflammatory cytokines may facilitate the resolution of inflammation in acute radiation-induced lung injury

Gaohua Han 1,*, Kaijin Lu 2, Wansong Xu 3, Sihui Zhang 1,*, Junxing Huang 1,✉, Chunlei Dai 4, Guangzhi Sun 3, Jun Ye 5
PMCID: PMC6540105  PMID: 31289503

Abstract

The present study evaluated the role of annexin A1 (ANXA1) in the treatment of acute radiation-induced lung injury (RILI) and investigated the mechanism of its action. The expression of ANXA1, interleukin-6 (IL-6) and myeloperoxidase (MPO) in the plasma of patients with RILI prior to and following hormonotherapy was assessed by enzyme-linked immunosorbent assay. The association of plasma ANXA1 concentration with clinical effect, and the correlation between the expression of ANXA1 and that of IL-6 and MPO were evaluated. ANXA1 was overexpressed or knocked down in a macrophage cell line, and its impact on IL-6 and MPO expression was measured. Following glucocorticoid hormonotherapy, patients with RILI exhibited a higher plasma concentration of ANXA1 compared with that prior to treatment, while IL-6 and MPO levels were lower. The concentration of ANXA1 in plasma was negatively correlated with IL-6 and MPO levels, with a correlation coefficient of −0.492 and −0.437, respectively (P<0.001). The increasing concentration of ANXA1 in plasma following treatment was associated with the clinical effect in patients with RILI (P=0.007). The expression levels of of IL-6 and MPO were inhibited both in the cytoplasm and in the culture solution, when ANXA1 expression was upregulated in a macrophage cell line. In conclusion, ANXA1 inhibited the synthesis and secretion of IL-6 and MPO inflammatory cytokines, indicating that ANXA1 may have therapeutic potential as a treatment target for RILI.

Keywords: annexin A1, interleukin-6, myeloperoxidase, radiation- induced lung injury

Introduction

Radiation-induced lung injury (RILI) is the most common dose-limiting side effect in patients with thoracic tumors following radiotherapy (1,2). Various strategies and drugs have been applied for the prevention and treatment of RILI, with poor therapeutic effect (3–6). Glucocorticoids have been administrated in numerous clinical studies for the treatment of acute RILI (1,7–9). However, prolonged usage of high doses of glucocorticoids can lead to a number of side effects, including osteoporosis, abnormal glucose metabolism, digestive tract ulcers and infection (10). Averting the side effects of radiotherapy without affecting the clinical benefits is an important goal.

The pathogenesis of RILI is complicated. Alveolar type II epithelial cells and lung capillary endothelial cells are the main targets of RILI (11,12), producing pro-inflammatory cytokines, including interleukin-6 (IL-6) (7) and transforming growth factor-β1 (TGF-β1) (13). Macrophages are the most important secretory cells of IL-6 and TGF-β1. In addition, macrophages also produce tumor necrosis factor-α (TNF-α), insulin-like growth factor 1, platelet-derived growth factor-β, macrophage-derived chemokines and other cytokines, These cytokines enhance the permeability of lung capillary endothelium and the chemotactic recruitment of monocytes, macrophages and neutrophils to the injured lung tissue in the target area. The inflammatory signal is amplified due to increased production of IL-6 and the generation of myeloperoxidase (MPO) by neutrophils, aggravating local inflammation and leading to damage of the alveolar structure (14–16). In addition, the secretion of TGF-β by macrophages can also lead to the progression of RILI into chronic pulmonary fibrosis. As a result, macrophages and neutrophils are influential in this process.

Annexin A1 (ANXA1) is a protein regulated by glucocorticoids. ANXA1 mimics the anti-inflammatory effect of glucocorticoids (17,18). ANXA1 is expressed in neutrophils, eosinophils and subcellular granules of monocytes and macrophages in the peripheral blood (19), and it inhibits the migration of neutrophils (20). Thus, ANXA1 has clinical potential for the treatment of RILI.

The role of ANXA1 in the pathogenesis of acute RILI and the mechanism of its clinical benefit on RILI are unclear. The present study evaluated the therapeutic potential of ANXA1 against RILI by assessing ANXA1 expression in the plasma of patients with RILI prior to and following 4 weeks of glucocorticoid treatment. The anti-inflammatory mechanism of ANXA1 was investigated by upregulating and downregulating ANXA1 expression in a macrophage cell line to assess the impact on IL-6 and MPO expression. Further understanding of the anti-inflammatory effect of ANXA1 on RILI may provide pre-clinical evidence to support targeting ANXA1 in the prevention and treatment of RILI.

Materials and methods

Subject information

A total of 50 patients with thoracic tumors suffering with RILI following radiotherapy between January 2014 and October 2016 at Taizhou People's Hospital (Taizhou, China) were included in the present study. The patients had experienced RILI with a severity of grade 2 or more after therapy, according to the Common Terminology Criteria for Adverse Events from the National Cancer Institute (version 3.0; National Institutes of Health, Rockville, MD, USA) (21). A total of 45 patients with complete clinical data were involved in the result analysis, including 25 males and 20 females, and the age ranging between 47 and 75 years old (median, 63.5 years). The research was approved by the Ethics Committee of Taizhou People's Hospital, and written informed consent was provided by each patient prior to the study.

Treatment of acute RILI and evaluation of treatment outcome

Glucocorticoid (methylprednisolone or dexamethasone) was administered to patients with RILI as the basic comprehensive treatment immediately after diagnosis. Methylprednisolone was administrated intravenously (iv), with a starting dose 1–2 mg/kg/day, and the dose would reduce by half every 4 days. Dexamethasone was administrated with a starting dose of 10 mg/day iv, and reduced as described previously. Routine blood examination was performed weekly during treatment, and a thoracic computed tomography scan was performed after 4 weeks of treatment. Response to treatment was assessed by alleviation of clinical symptoms (e.g. cough, fever, chest distress, dyspnea) and imaging (X-ray, CT scan) and laboratory findings (white blood cell count). The treatment outcome was evaluated comprehensively based on the patients' clinical symptoms and the aforementioned indicators, including CT scan and white blood cell count and neutrophils count.

Neutrophil count and ELISA

Peripheral venous blood was drawn from each patient weekly from the time of diagnosis until 1 month post-treatment. Then, the neutrophil count was measured through routine blood tests, and the expression of ANXA1, IL-6 and MPO was assessed using ELISA in the plasma extracted by centrifugation from the blood samples. The ANXA1 (cat. no. ab222868; antibody name: AF3770) and IL-6 (cat. no. PD6050; antibody name: MAB206) kits, and the primary antibody were purchased from R&D Systems China Co., Ltd. The MPO kit was purchased from Abcam Shanghai Co., Ltd. (cat. no. ab119605; antibody name: ab45977). Measurements were performed in triplicate for each sample. The absorbance was read at 450 nm and the protein levels were calculated based on the standard curve. Blood samples from 20 healthy donors were also assessed as the negative control.

Plasmid construction for ANXA1 expression

Eca109 human esophageal cancer cells, purchased from Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences were cultured in DMEM with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and antibiotics at 37°C with 5% CO2. RNA from Eca109 human esophageal cancer cells was extracted using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc., Shanghai, China). The AMV reverse transcription kit (Promega Corporation) was subsequently used for reverse transcription to obtain the cDNA which was used as a template, together with ANXA1 primers (synthesized by Invitrogen; Thermo Fisher Scientific, Inc. Shanghai, China) for PCR amplification: The primer sequences were as follows: Sense, 5′-ATGGCAATGGTATCAGAATTCCTC-3′ and antisense, 5′-TTAGTTTCCTCCACAAAGAGCCACC-3′. PrimerSTAR HS DNA polymerase (Takara Biotechnology Co., Ltd., Dalian, China) was used for amplification. The reaction parameters were as follows: 95°C ×3 min, 94°C ×30 sec, 58°C ×30 sec, 72°C ×5 min, 72°C ×5 min, for 27 cycles. The PCR product was purified using the QIAquick PCR Purification kit (Qiagen China Co., Ltd., Shanghai, China). ANXA1 cDNA was cleaved with the restriction endonucleases XhoI and BamHI (Fermentas; Thermo Fisher Scientific, Inc., Pittsburgh, PA, USA) and ligated with the pIRES2-EGFP vector (Takara Biotechnology Co., Ltd.) to form a pIRES2-EGFP-ANXA1 (pIRES2-ANXA1) expression plasmid. The plasmid was expressed in competent Escherichia coli DH5α cells (Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences) and purified using a mini-prep kit from Axygen Biosciences (Union City, CA, USA). The construct was examined by enzyme digestion and sequenced as previously described (22).

Differentiation and cultivation of M2 macrophages

Human peripheral-blood monocytes are used as an established in vitro system for generating macrophages, and monocytic cell lines, as THP-1 cells have been considered as a possible alternative (23–25). In the present study, THP-1 cells were acquired from the Shanghai Institute of Biochemistry and Cell Biology, and were cultured in RPMI-1640 medium (Thermo Fisher Scientific, Inc.) supplemented with non-heat-treated 10% FBS (Invitrogen; Thermo Fisher Scientific, Inc.), 2 mM L-glutamine, 0.05 mM β-mercaptoethanol, 4,500 mg/l glucose, 100 U/ml penicillin and 100 µg/ml streptomycin at 37°C with 5% CO2. THP-1 cells were kept at a minimum density of 5×105 cells/ml and were passaged when reaching 5×106 cells/ml. The cells were induced to differentiate into M2 macrophages by PMA (Sigma-Aldrich; Merck KGaA) at a final concentration of 150 ng/ml and IL-4 at a final concentration of 20 ng/ml incubated for 48 h. The cells were imaged using an optical microscope. Differentiation was verified by assessing expression of a marker of the M2 phenotype, macrophage mannose receptor 1 (CD206) (26–28). Cells were collected at 48 h after induction for detection. The induced cells were digested with trypsin and collected by centrifugation at 200 × g for 10 min at 4°C (induction group). Uninduced cells were centrifuged, as described previously, and collected (control group). The pellet was resuspended in PBS and the cells were washed with PBS solution twice. Then, the cells were suspended at ~1×106 cells/ml in PBS for further use. The Phycoerythrin (PE)-conjugated anti-CD206 monoclonal antibody (dilution, 1:20; cat. no: 555954; BD Pharmingen) was added to the cell suspension, which was subsequently incubated in the dark for 30 min at 4°C. After two washes in PBS, the cells were resuspended with 2% paraformaldehyde (in cold PBS) and incubated for 20 min at 4°C. Suspension was centrifuged at 200 × g for 5 min at 4°C and the pellet resuspended with glycine 0.1 M (in cold PBS) and incubated for 10 min at 4°C. Then, the last centrifugation of 5 min at 200 × g at 4°C was performed before cell resuspension in 1 ml of PBS and the expression of CD206 was measured using a flow cytometer (FACScalibur; BD Biosciences), and analyzed by the FlowJo 7.6.4 software (TreeStar Inc.).

Overexpression and inhibition of ANXA1 in THP-1 cells differentiated into M2 macrophages

THP-1 cells were induced into macrophage 48 h after transfection. The pIRES2-ANXA1 expression plasmid was transfected into THP-1 cells using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.). The pIRES2-EGFP empty plasmid was transfected as the control. Prior to transfection, the plasmid (1 µg/ul) was mixed with liposomes and allowed to stand for 20 min. The mixture was dispensed into culture wells (2 µg DNA and 5 ul liposomes per well) following the manufacturer's protocol. The transfection efficiency was assessed by fluorescence microscopy and western blot analysis. The transfected THP-1 cells were then induced to generate ANXA1-overexpressing macrophages. Macrophages harboring the ANXA1 plasmid comprised the pIRES2-ANXA1 group and those with the empty plasmid comprised the no-load group. Non-transfected macrophages comprised the blank control. Each group of cells was partitioned into 3 wells and the experiments were repeated 3 times.

Small interfering RNAs (siRNAs) against ANXA1 were transfected into THP-1 cells to inhibit the expression of ANXA1. The siRNAs included 2 sequences targeting ANXA1 and a scrambled control sequence as follows: siRNA (a), 5′-CAGCGUCAACAGAUCAAAG-3′; siRNA (b), 5′-CCGAUCUGAGGACUUUGGU-3′; and control siRNA, 5′-CAGUCGCGUUUGCGACUGG-3.′ These siRNA sequences were designed as previously described (29). The siRNAs were synthesized by Shanghai Sangon Company (Shanghai, China). After 48 h these transfected THP-1 cells were then induced to differentiate into macrophages. Macrophages transfected with siRNA (a) and siRNA (b) were designated as the siRNA (a) and siRNA (b) groups, respectively. Macrophages transfected with control siRNA or plasmid were designated as the siRNA (control) group or the no-load group, respectively. Non-transfected macrophages comprised the blank control group.

Western blot analysis

Macrophages in each well were trypsinized and total cell protein was extracted using cell lysis buffer (cat. no. P0013; Beyotime Institute of Biotechnology). Sample protein concentrations were determined for each group using the DC Protein assay kit (Bio-Rad Laboratories, Inc., Hercules, CA, USA). SDS-PAGE (15%) was performed for each group using 50 µg protein sample per lane, and proteins were then transferred from the gel to polyvinylidene difluoride membranes (EMD Millipore, Billerica, MA, USA). Following a 1-h incubation in blocking buffer (cat. no. C-0042; 5% bovine serum albumin; Xiamen, BOYAO Biotechnology Co., Ltd.) at 4°C, membranes were incubated with the following primary antibodies at 4°C overnight with agitation: Anti-IL-6 (1:1,000; cat. no. RAB0307; Sigma-Aldrich; Merck KGaA), anti-ANXA1 (1:1,000; cat. no. SAB1405457; Sigma-Aldrich; Merck KGaA), anti-MPO (1:1,000; cat.no. 76923; Cell Signaling Technology, Inc., Danvers, MA, USA) and anti-GAPDH (1:3,000; cat.no. sc-47724; Santa Cruz Biotechnology, Inc., Dallas, TX, USA). Following washing with stripping buffer [β-mercaptoethanol 342 µl; 20% SDS 5 ml; Tris-Cl (pH 6.7) 3.125 ml; ddH2O 41.533 ml to total volume 50 ml], the membranes were incubated with agitation at room temperature for 1 h with a secondary antibody (m-IgGκ BP-HRP: sc-516102;Santa Cruz Biotechnology, Inc.) labeled with horseradish peroxidase in the presence of 2% bovine serum albumin (Invitrogen; Thermo Fisher Scientific, Inc), then HRP was visualized by Chemiluminescent Western Blot Detection kit (SuperSignal West Femto; Thermo Fisher Scientific, Inc.). The membranes were washed and imaged, and the bands were analyzed semi-quantitatively with ImageJ 1.8 software (https://imagej.nih.gov/ij/download.html).

Effect of altered expression of ANXA1 on expression of IL-6 and MPO

M2 macrophages in which ANXA1 was overexpressed or inhibited were resuspended for cell counting following transfection to ensure that the cell density in each well for further cultivation was the same. Following 72 h in culture, ELISA was performed on the medium to evaluate the level of IL-6 and MPO secreted by the macrophages, and western blot analysis was performed to evaluate the levels of IL-6 and MPO protein in the macrophages. ELISA was performed as aforementioned for peripheral venous blood samples.

Statistical analysis

Measurement data are presented as the mean ± standard deviation. SPSS (version 19.0; IBM Corp., Armonk, NY, USA) was utilized to create the database. Differences between means of two groups were compared using independent-samples t-test and those among multiple groups were compared using one-way analysis of variance (ANOVA). ANOVA was used to compare the means among baseline group and 1, 2, 3 and 4 weeks after treatment groups. Then, the Least Significant Difference post hoc test was used to compare between every two groups. Associations between treatment outcome and ANXA1, IL-6 and MPO levels were compared using a χ2 test. Correlation analysis was performed using Spearman's rank correlation method. P<0.05 was considered to indicate that a difference was statistically significant.

Results

ANXA1, IL-6 and MPO levels in the plasma of patients with RILI

Among the 45 cases with RILI, the baseline level of ANXA1, IL-6 and MPO in the plasma prior to glucocorticoid treatment was 317.35±191.06, 258.45±172.32 pg/ml and 139.24±57.94 ng/ml, respectively. The corresponding level of healthy control group was 22.75±9.56, 41.05±12.33 pg/ml and 62.24±27.39 ng/ml, respectively. There was significant difference of ANXA1, IL-6 and MPO between the baseline of RILI patients and the healthy control (P<0.001) Following glucocorticoid administration, the expression of ANXA1 increased gradually (ANOVA test, F=31.503; P<0.001). The post hoc test showed that ANXA1 of 1, 2, 3 and 4 weeks after treatment was significantly higher compared with that of baseline, (P=0.003, <0.001, <0.001, <0.001, respectively), while that of IL-6 and MPO decreased, and the number of neutrophils remained unchanged (Fig. 1). Spearman's correlation analysis indicated that the ANXA1 level in the plasma following glucocorticoid treatment was significantly negatively correlated with the levels of IL-6 and MPO, with a correlation coefficient of −0.974 and −0.956, respectively (P<0.001; Fig. 2).

Figure 1.

Figure 1.

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ANXA1, IL-6, MPO and neutrophils in the plasma of patients with acute radiation-induced lung injury following glucocorticoid administration for 4 weeks. ANXA1 at 4 weeks was significantly higher compared with the baseline level (317.35 vs. 586.75 pg/ml; P<0.001). IL-6 decreased gradually, and was significantly lower at 4 weeks compared with the baseline (258.45 vs. 139.35 pg/ml; P=0.003). The neutrophil count did not change with glucocorticoid administration (33.5×109 vs. 31.78×109 cells/dl; P=0.247). MPO decreased and was notably lower compared with the baseline level following 4 weeks of glucocorticoid treatment (139.24 vs. 51.35 ng/ml; P=0.018). ANXA1, annexin A1; IL-6, interleukin 6; MPO, myeloperoxidase; GRA, granulocyte count, absolute.

Figure 2.

Figure 2.

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The correlation between the expression level of ANXA1 and IL-6 or MPO in plasma of patients with RILI treated with glucocorticoid. (A) Negative correlation between ANXA1 and IL-6; r=−0.974. (B) Negative correlation between ANXA1 and MPO, r=−0.956. ANXA1, annexin A1; IL-6, interleukin 6; MPO, myeloperoxidase; RILI, radiation-induced lung injury.

Association between treatment outcome and expression levels of ANXA1, IL-6 and MPO in patients with RILI

Among the 45 cases, recovery occurred in 20 patients 2 weeks after glucocorticoid treatment, and a further 11 patients with repeat symptoms were cured following 4 weeks of treatment. These 31 cases were considered to have had an efficient treatment outcome. Interstitial lung diseases combined with chronic lung infection occurred in the remaining 14 cases, which were considered to have progressive disease. Cases were grouped by these two outcomes-efficient or progressive group. According to the increase of ANXA1 after hormone treatment, patients were divided into two groups: Patients with more than twice the baseline level and patients with less than two times the baseline level. Similarly, patients were divided into two groups according to the decrease of IL-6 and MPO, which were less than half of the baseline level and more than half of the baseline level group. Then, χ2 testing was performed to compare treatment outcome to expression levels of ANXA1, IL-6 and MPO in plasma following glucocorticoid treatment. The results indicated that the expression levels of ANXA1, IL-6 and MPO were associated with the treatment outcome for RILI. The increased expression of ANXA1 following 4 weeks of treatment was associated with improved treatment outcome for RILI (P=0.007), while decreased expression of IL-6 and MPO expression following 4 weeks of treatment was associated with improved treatment outcome (P=0.042 and P=0.003, respectively) (Table I).

Table I.

Association between the clinical effect against RILI and the plasma levels of ANXA1, IL-6 and MPO at 4 weeks of glucocorticoid treatment.

ANXA1 in plasma IL-6 in plasma MPO in plasma
Clinical effect against RILI >2× baseline level <2× baseline level <50% of baseline level >50% of baseline level <50% of baseline level >50% of baseline level
Efficient, n 20 11 19 12 19 12
Progressive, n 3 11 4 10 2 12
χ2 7.166 4.132 8.562
P-value 0.007 0.042 0.003
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RILI, radiation-induced lung injury; ANXA1, annexin A1; IL-6, interleukin 6; MPO, myeloperoxidase.

Differentiation of THP-1 cells and transfection of p-IRES2-ANXA1

PMA and IL-4 were added to the culture medium of THP-1 cells to induce differentiation to an M2 macrophage phenotype. THP-1 cells were round or quasi-circular, uniform in size and suspended in culture medium (Fig. 3A) and became adherent, spindle-shaped or polygonal cells (Fig. 3B) after 48 h in the culture medium with PMA and IL-4, which expressed CD206 (Fig. 3C) in flow cytometry. These results indicated that THP-1 cells were successfully induced to differentiate into M2 macrophages. Successful ANXA1 plasmid transfection indicated by green fluorescence was observed by fluorescence microscopy in the pIRES2-ANXA1 group of cells (Fig. 3D). A similar appearance was noted in the no-load group. Green fluorescence was absent in the blank control group. The results of the present study demonstrated that THP-1 cells transfected with p-IRES2-ANXA1 plasmid could differentiate into M2 macrophages, which enabled stable expression of ANXA1.

Figure 3.

Figure 3.

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Differentiation of THP-1 cells to M2 macrophages. (A) THP-1 cells were circular or quasi-circular, growing in a state of suspension (magnification, ×200). (B) THP-1 cells induced with phorbol 12-myristate 13-acetate and interleukin-4 became polygonal shape with outgrowth, and grew adherent to the plate wall (magnification, ×200). (C) CD206 expression was observed by flow cytometry 48 h after induction. (D) Green fluorescent protein in macrophages transfected with pIRES2-ANXA1: The cells were spindle-like or pleomorphic (magnification, ×200). ANXA1, annexin A1; CD206, macrophage mannose receptor 1; PE, phycoerythrin.

Expression of ANXA1 in M2 macrophages following transfection

Following transfection of M2 macrophages with the p-IRES2-ANXA1 plasmid or siRNA against ANXA1, western blot analysis was used to determine ANXA1 protein expression. The cells transfected with pIRES2-ANXA1 exhibited a stronger ANXA1 signal compared with the cells in the blank control and no-load group. In macrophages transfected with siRNA (a) and siRNA (b), the ANXA1 band was weaker compared with that of the blank control and siRNA (control) groups (Fig. 4).

Figure 4.

Figure 4.

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Expression of ANXA1 and GAPDH in untransfected macrophages (blank control) or macrophages transfected with pIRES2-GFP (No-load), pIRES2-ANXA1, siRNA (a), siRNA (b) or siRNA (control). ANXA1, annexin A1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; siRNA, short interfering RNA.

Effect of ANXA1 expression on IL-6 protein expression and secretion in macrophages

Western blot results revealed decreased IL-6 protein expression in macrophages transfected with p-IRES2-ANXA1, while ANXA1 inhibition caused by siRNA led to enhanced IL-6 expression (Fig. 5A). The concentration of IL-6 in the culture medium exhibited a similar changing pattern, with a mean concentration of 215.23±42.11 pg/ml in the blank control group, 103.62±28.26 pg/ml in the ANXA1 overexpression group, 271.73±67.83 pg/ml in the siRNA (a) group and 264.24±55.97 pg/ml in the siRNA (b) group (Fig. 5B). The concentration of IL-6 in the culture medium of the siRNA (control) and no-load group was 217.71±52.34 and 209.65±42.15 pg/ml, respectively, which was similar to that in the blank control group, indicating that ANXA1 inhibited the synthesis of IL-6 in macrophages and subsequent secretion.

Figure 5.

Figure 5.

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IL-6 and MPO expression in M2 macrophages transfected with pIRES2-ANXA1 or siRNA against ANXA1, siRNA (a) and siRNA (b). (A) IL-6 protein expression was decreased in macrophages with overexpression of ANXA1, while ANXA1 inhibition induced by siRNA led to increased IL-6 expression. (B) IL-6 in culture medium was decreased with overexpression of ANXA1 (*P<0.05 compared with blank control) and increased with siRNA-mediated ANXA1 inhibition (**P<0.05 and ***P<0.05, compared with blank control). (C) MPO protein expression was decreased in macrophages with overexpression of ANXA1, while ANXA1 inhibition induced by siRNA led to increased IL-6 expression. (D) MPO in cell culture medium was decreased with overexpression of ANXA1 («P<0.01, compared with blank control), and increased with siRNA-mediated ANXA1 inhibition (««P<0.05 and «««P<0.05 compared with blank control). ANXA1, annexin A1; IL-6, interleukin 6; MPO, myeloperoxidase; siRNA, short interfering RNA.

Effect of ANXA1 expression on protein expression and secretion of MPO

MPO protein expression decreased in macrophages transfected with p-IRES2-ANXA1, while ANXA1 inhibition enhanced MPO protein expression (Fig. 5C). ELISA results demonstrated a similar pattern of MPO expression in the culture medium: 175.32±42.35 pg/ml in the blank control group, 73.04±15.06 pg/ml in the ANXA1 overexpression group, 214.15±59.37 pg/ml in the siRNA (a) group and 221.21±64.87 pg/ml in the siRNA (b) group (Fig. 5D). The respective levels in the siRNA (control) and no-load group (167.51±38.26 and 171.92±50.07 pg/ml) were similar to that in the blank control group, indicating that ANXA1 inhibited the synthesis of MPO in macrophages and subsequent secretion.

Discussion

To date, efforts to treat acute RILI have focused on neutralizing pro-inflammatory cytokines or attenuating the infiltration of inflammatory cells (5). These efforts have been hampered by a lack of knowledge of the mechanisms of the cytokine or signaling pathways involved in the pathogenesis of RILI. Thus, no standard countermeasure or specific drug with a definite target has been developed. ANXA1 is a member of the calcium-dependent, phospholipid binding protein superfamily, which was first discovered to be a secondary messenger downstream of the glucocorticoid receptor signaling pathway (30). ANXA1 is critical in the induction and regulation of anti-inflammatory treatment using glucocorticoids. ANXA1 also takes part in regulating phagocytosis, differentiation, proliferation, apoptosis and signaling transduction (31). Additionally, several animal model studies demonstrated the anti-inflammatory effect of ANXA1 (32,33). Treatment of acute RILI using ANXA1 has not been described and its roles in RILI pathogenesis remain unclear.

The present study observed that glucocorticoid treatment is associated with increased ANXA1 in plasma. An increased concentration of ANXA1 in plasma following glucocorticoid treatment was associated with a more positive treatment outcome for RILI, indicating that ANXA1 may serve a beneficial role in the treatment of acute RILI.

ANXA1 in the plasma of patients with RILI was negatively correlated with the plasma levels of IL-6 and MPO, implying that ANXA1 may impact RILI treatment outcome by influencing the expression of these pro-inflammatory cytokines. IL-6 is an important pro-inflammatory factor, which is synthesized by activated alveolar macrophages, T helper 2 cells, lung fibroblasts and alveolar type II epithelial cells. IL-6 is involved in the responses to lung injury and early-stage inflammation (34), and promotes the transition from CD4+ cells to T helper 17 cells, facilitating the accumulation and activation of neutrophils within the inflammatory area (35). MPO is mainly expressed by neutrophils and macrophages. During inflammation, neutrophils are activated and degranulated, and MPO is secreted into extracellular matrix.

Extracellular MPO is an alkaline protein with positive charge, which is easily adsorbed on the surface of negatively charged cell membrane (36). Hydrogen dioxide (H2O2) in extracellular matrix can aggregate to the site where the two bind, thus causing tissue damage (37). In addition, MPO is absorbed by vascular endothelial cells after being release from neutrophils, leading to vasculitis and further aggravating tissue damage (38). It is well known that radiotherapy can lead to the ionization of water molecules in the target area to produce H2O2, so reactions catalyzed by MPO lead to oxidative injury and enhanced radiation-induced injury to the target tissue (39). In the present study, the baseline levels of IL-6 and MPO in RILI patients prior to glucocorticoid treatment were higher compared with those in the healthy controls, and the expression of the two factors was positively correlated. These observations are consistent with the previous literature (40,41). The present findings support a previous report indicating that ANXA1 overexpression results in downregulation of IL-6 (42). A similar correlation between ANXA1 and IL-6 was also observed following fibroblastoma irradiation treatment (43). ANXA1 can induce apoptosis in neutrophils (44), and inhibit neutrophil migration and accumulation in the inflammatory area (45). In the present study, the expression of ANXA1 was increased in patients with RILI without an evident reduction of neutrophils in the circulation system, but with a significant reduction in the level of MPO. A similar result has been reported in a previous study (46). In a further study, more apoptotic neutrophils were observed when the observation time frame was elongated, possibly due to the impaired function of the releasing granules of neutrophils following 4 weeks of increased ANXA1 expression, when no evident apoptosis was observed (47).

In the present study, ANXA1 was increased in the plasma of patients with acute RILI following glucocorticoid therapy. These results are consistent with several reports (22,48). The present study also demonstrated that higher ANXA1 expression was associated with a beneficial treatment outcome for RILI following glucocorticoid administration, indicating an increased anti-inflammatory effect. A prior study reported that the anti-inflammatory effect of glucocorticoid was associated with induced expression of ANXA1 (49). Further studies demonstrated that ANXA1 gene knockdown in a mouse model of inflammation markedly upregulated the pro-inflammatory cytokine IL-6 (50), and an anti-inflammatory effect could not be achieved with glucocorticoid administration (51). Similar results were acquired when an antibody against ANXA1 was applied to inhibit its function (52). These results collectively demonstrate that the anti-inflammatory effect of glucocorticoid depends on the induction of ANXA1 expression, highlighting the potential of this protein as an anti-inflammatory therapy without the use of hormones.

In the present study, the overexpression of ANXA1 in macrophages inhibited the synthesis and secretion of IL-6 and MPO, while the inhibition of ANXA1 promoted these events, indicating that ANXA1 could serve an anti-inflammatory role, as previously reported (19,44). ANXA1 alone has been reported to exert an anti-inflammatory effect. The ANXA1 N-terminal-derived peptide Ac2-26 can inhibit local and systematic inflammatory responses (53). Induction of compound 43, an agonist of the ANXA1 receptor, inhibits the expression of IL-6 in patients with rheumatoid arthritis and ameliorates bone damage by reducing the infiltration of inflammatory cells in synovial tissues (54). ANXA1 inhibits the activity of calcium-activated, phospholipid-dependent membrane-bound enzymes, particularly phospholipase A2 (55,56). ANXA1 and its N-terminal fragment were also demonstrated to inhibit the synthesis of arachidonic acid derivatives (57,58). ANXA1 restrains the inflammatory response induced by tumor necrosis factor-α by suppressing Rac-1-dependent NADPH oxidase in vascular epithelial cells (59). A more recent study demonstrated that ANXA1 could exert anti-inflammatory effects by inhibiting the transcriptional activity of nuclear factor-κB (NF-κB), as ANXA1 binds specifically to the p65 subunit of NF-κB, blocking binding to its target genes (49). However, conflicting findings have been reported. In one study, ANXA1 was found to promote inflammation by facilitating the transendothelial migration of neutrophils and monocytes (60). ANXA1 has also been demonstrated to mediate the activation of the extracellular signal-regulated kinase, c-Jun N-terminal kinase and NF-κB inflammatory pathways (61). Notably, one study indicated a role of endogenous ANXA1 in the anti-inflammatory effect, while exogenous ANXA1 induced inflammation (62). It is clear that more studies are required to clarify the exact role of ANXA1 in inflammation and RILI pathogenesis.

In conclusion, ANXA1 reduces the expression of IL-6 and inhibits the release of MPO from monocytes and macrophages. This anti-inflammatory effect may underscore the mechanism by which ANXA1 in the plasma of patients with RILI is associated with treatment outcome. Although glucocorticoids are common drugs in RILI treatment, the present study indicates the potential of ANXA1 to mimic the anti-inflammatory effect of glucocorticoids, which would potentially avoid certain adverse effects of this therapy.

Acknowledgements

Not applicable.

Funding

This study was supported by the Scientific Program of the Health and Family Planning Commission of Jiangsu Province (grant No. H2017076) and the Foundation of Jiangsu Provincial Medical Innovation Team (grant No. CXTDA2017042).

Availability of data and materials

All data generated or analyzed during the present study are included in this published article.

Authors' contributions

GH and JH conceived and were responsible for the design of the present study. GS, WX and KL analyzed and interpreted the patients' data. CD was responsible for the diagnosis of RILI and assessment of the curative effect based on imaging examinations. SZ and JY analyzed and interpreted the laboratory data. GH and SZ were the major contributors in writing the manuscript. All authors read and approved the present manuscript.

Ethics approval and consent to participate

This research was approved by the Ethics Committee of Taizhou People's Hospital, and written informed consent was provided by each patient prior to the study.

Patient consent for publication

All patients signed an informed consent for publication approved by the institutional Review Board.

Competing interests

The authors declare that they have no competing interests.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Data Availability Statement

All data generated or analyzed during the present study are included in this published article.

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