Table 5.
Experimental chemicals and reagents.
| Chemicals and Reagents | Sources |
|---|---|
| Recombinant human TNF-α protein | Novoprotein (Shanghai, China) |
| Sensor chips (CM 5) | GE Healthcare Life Science (Uppsala, Sweden) |
| Immobilization buffer (acetate to pH levels of 5.5, 5.0, 4.5, and 4.0) | |
| PBS-P buffer (10 mM phosphate buffer containing 137 mM NaCl, 2.7 mM KCl, and 0.05% surfactant P20, with a pH of 7.4) | |
| Regeneration solutions (10 mM NaOH) | |
| Amine Coupling Kit (EDC and NHS; 1.0 M ethanolamine (pH of 8.5)) | |
| Glycine 2.0 | |
| Methanol (HPLC grade) | Fisher Scientific (Pittsbargh, PA, USA) |
| Water | Hangzhou Wahaha group (Hangzhou, China) |
| Formic acid | Dikma Co. (Richmond Hill, NY, USA) |
| Internal standards (chloramphenicol) (purity ≥99.0%) | Sigma (St. Louis, MO, USA) |
| 3,4-dihydroxybenzoic acid (purity ≥99.0%) | Chengdu Must Bio-technology Co., Ltd. (Chengdu, China) |
| Caffeic acid (purity ≥99.0%) | |
| 3-O-caffeoylquinic acid (purity ≥99.0%) | |
| 4-O-caffeoylquinic acid (purity ≥99.0%) | |
| 5-O-caffeoylquinic acid (purity ≥99.0%) | |
| 3,5-O-di-caffeoylquinic acid (purity ≥99.0%) | |
| 3,4-O-di-caffeoylquinic acid (purity ≥99.0%) | |
| 4,5-O-di-caffeoylquinic acid (purity ≥99.0%) | |
| Rutin (purity ≥ 99.0%) | |
| Luteoloside (purity ≥ 99.0%) |