Volume 306, May 1, 2014
Volume 75, May 1, 2014
Pages F1059–F1068. Rengarajan S, Lee DH, Oh YT, Delpire E, Youn JH, McDonough AA. Increasing plasma [K+] by intravenous potassium infusion reduces NCC phosphorylation and drives kaliuresis and natriuresis. Am J Physiol Renal Physiol 306: F1059–F1068, 2014. First published March 5, 2014; doi: https://doi.org/10.1152/ajprenal.00015.2014.—On p. F1060, under Homogenate Preparation, the first sentence, containing the buffer composition, was printed incorrectly. The correct version appears below.
As described (28), cortex and medulla were separated by dissection, homogenized [5% sorbitol containing 5 mM histidine-imidazole (pH 7.5), 0.5 mM Na2EDTA, 9 μg/ml aprotinin, 0.2 mM PMSF, and 5 μl/ml of a phosphatase inhibitor cocktail (Sigma P0044)] with an Ultra-Turrax T25 (IKA-Labortechnik) at a low setting for 5 min, and centrifuged at 2,000 g for removal of debris, and the supernatant was retained, the pellet was rehomogenized, and both supernatants (2,000 g supernatant = “homogenate”) pooled, quick frozen, stored at −80°C.
