Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 4;38(22):4397–4411. doi: 10.1038/s41388-019-0730-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Global identification of sGC-regulated genes in TMPRSS2-ERG positive PCa cells. a–c VCaP cells transfected with siNTC or siRNA against GUCY1B1/A1 (siB1/siA1) individually or in combination were subjected to a immunoblotting, b real-time RT-PCR, or c cGMP ELISA assay with/out riociguat stimulation (12 h). d, e VCaP cells transfected with siNTC, siA1, or siB1 were subjected to RNA-seq analysis. d Venn diagram for overlapping or differentially regulated genes by GUCY1B1/A1 (cutoff 1.5-fold) and e KEGG pathway analyzes on GUCY1B1 or A1 regulated genes were presented. f A panel of GUCY1B1 upregulated genes were selected for subsequent validation. VCaP cells transected with siNTC, siA1 or siB1 (upper panel) or VCaP cells treated with NS2028 or KT5823 (1d) (lower panel) were subjected for real-time RT-PCR analyzes