Figure 4.

Transgenic EcR-B1 Rescues Defective Axon Pruning Associated with Loss of Kis

(A–D) Representative images of MARCM-generated MB clones expressing membrane-bound GFP using the 201y-Gal4 driver 18–22 h APF. (A) control (w¹¹¹⁸; FRT40A), (B) kis null mutant (Kis^LM27,FRT40A), (C) EcR-B1 overexpression (FRT40A; UAS:EcR-B1), and (D) EcR-B1 rescue (Kis^LM27,FRT40A; UAS:EcR-B1).

(E) Quantification of dorsal, medial, and total MB lobe surface areas in MARCM animals (from left to right, n = 12, 11, 10, 12 MBs).

(F–Q) Representative adult MARCM-generated MB clones expressing membrane-bound GFP or stained with α-FasII. (F) control (w¹¹¹⁸; FRT40A) membrane-bound GFP, (G) α-FasII immunostaining, and (H) merge images. (I) kis null mutant (Kis^LM27,FRT40A) GFP, (J) α-FasII, and (K) merge. (L) EcR-B1 overexpression alone (FRT40A; UAS:EcR-B1) GFP, (M) α-FasII immunostaining, and (N) merge. (O) EcR-B1 rescue (Kis^LM27,FRT40A; UAS:EcR-B1) GFP, (P) α-FasII immunostaining, and (Q) merge. Arrows indicate aberrant axonal projections in (I) and (K). Insets show magnified area of medial lobes.

(R) Quantification of average aberrant axonal projections in MBs (from left to right, n = 13, 12, 8, 10 MBs).

Scale bars: 10 μm in (A) and 20 μm in (F). Statistical significance is represented by * = p < 0.05, ** = p < 0.01, and *** = p < 0.001. Error bars represent the SEM.