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. 2019 Jan 24;104(6):1189–1201. doi: 10.3324/haematol.2018.202044

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Figure 3. — Co-expression of MA4 and A4M enhances hematopoietic differentiation of human embryonic stem cells in OP9 co-culture. (A) Experimental design of OP9-based human embryonic stem cell (hESC) differentiation towards hemato-endothelial precursors (HEP) and further hematopoietic commitment of HEP maintained in either liquid culture for 16 days or in MS5 co-culture for 30 days. (B) Frequency of total CD45⁺ blood cells after 9 days in OP9 co-culture. (C,D) CD45-CD31⁺CD34⁺ HEP were FACS-purified at day 9 of OP9 co-culture and allowed to differentiate into CD45⁺ cells in liquid culture (C) or in MS5 co-culture (D). Data are represented as the mean ± standard error of mean from independent experiments. bFGF: basic fibroblast growth factor; MTG: monothioglycerol; SCF: stem cell factor; IL: interleukin; G-CSF: granulocyte colony-stimulating factor; EV: empty vector.