Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus. musculus) | B6.Cg-Tg(S100a4-EGFP)M1Egn/YunkJ (S100A4-GFPpromoter) | Jackson Laboratory | Stock #: 012893 RRID: MGI:4819362 |
|
| Genetic reagent (M. musculus) | B6.Cg-Tg(S100a4-TK)M31Egn/YunkJ (S100a4-TK) | Jackson Laboratory | Stock #: 012902 RRID:MGI:4454768 |
|
| Genetic reagent (M. musculus) | B6.129S6-S100a4tm1Egn/YunkJ (S100a4GFP/+) | Jackson Laboratory | Stock #: 012904 RRID:MGI:4819358 |
|
| Genetic reagent (M. musculus) | BALB/c-Tg(S100a4-cre)1Egn/YunkJ (S100a4-Cre) | Jackson Laboratory | Stock #: 012641 RRID:MGI:4454332 |
|
| Genetic reagent (M. musculus) | B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (ROSA-Ai9) | Jackson Laboratory | Stock #: 007909 RRID:MGI:3809523 |
|
| Genetic reagent (M. musculus) | Tg(Csf1r-Mer-iCre-Mer)1Jwp (Csf1r-iCre) | Jackson Laboratory | Stock #: 019098 RRID:IMSR_JAX:019098 |
|
| Genetic reagent (M. musculus) | C57BL/6J | Jackson Laboratory | Stock #: 000664 RRID:MGI:3028467 |
|
| Antibody | anti-RAGE (mouse monoclonal) | Santa Cruz Biotechnology | Cat. #: sc-365154 RRID:AB_10707685 |
1:100 |
| Antibody | anti-F4/80 (goat polyclonal) | Santa Cruz Biotechnology | Cat. #: sc-26642 RRID:AB_2098333 |
1:500 |
| Antibody | anti-GFP (goat polyclonal) | Abcam | Cat. #: ab6673 RRID:AB_305643 |
1:5000 |
| Antibody | anti-RFP (rabbit polyclonal) | Abcam | Cat. #: ab62341 RRID:AB_945213 |
1:500 |
| Antibody | anti-S100a4 (rabbit monoclonal) | Abcam | Cat. #: ab197896 RRID:AB_2728774 |
1:20000 |
| Antibody | anti-iNOS (rabbit polyclonal) | Abcam | Cat. #: ab15323 RRID: AB_301857 |
1:100 |
| Antibody | Anti-ILIRa (rabbit monoclonal) | Abcam | Cat. #: ab124962 RRID:AB_11130394 |
1:10000 |
| Antibody | anti-alpha-SMA-Cy3 (mouse monoclonal) | Sigma-Aldrich | Cat. #: C6198 RRID: AB_476856 |
1:250 |
| Antibody | Donkey anti-rabbit AlexaFluor594 secondary | Jackson ImmunoResearch | Cat. #: 711-585-152 RRID: AB_2340621 |
1:200 |
| Antibody | Donkey anti-rabbit 647 secondary | Jackson ImmunoResearch | Cat. #: 711-605-152 RRID: AB_2492288 |
1:200 |
| Antibody | Donkey anti-rabbit Rhodamine-Red-X | Jackson ImmunoResearch | Cat. #: 711-296-152 RRID:AB_2340614 |
1:100 |
| Antibody | Donkey anti-goat 488 secondary | Jackson ImmunoResearch | Cat. #: 705-546-147 RRID: AB_2340430 |
1:200 |
| Antibody | Goat anti-mouse AlexaFluor488 secondary | ThermoFisher | Cat. #: A11029 RRID:AB_138404 |
1:1000 |
| Chemical compound, drug | Nucleoside analog ganciclovir (GCV) | TSZCHEM | Cat #: 82410-32-0 | 75 mg/kg |
| Peptide, recombinant protein | RAGE Antagonist Peptide (RAP) | MilliporeSigma | Cat. #: 553031 | 100 ug (peptide) |
| Peptide, recombinant protein | Human S100a4 Recombinant protein | LSBio | Cat #: G1305 | 20–1000 ng/mL (recombinant protein) |
| Commercial kit | Rabbit polymer kit | Vector Laboratories | Cat #: MP-7401 | |
| Software | OlyVIA software | Olympus (https://www.olympus-lifescience.com/en/support/downloads/) | RRID:SCR_016167 | Version 2.9 |
| Software | ImageJ software | ImageJ (http://imagej.nih.gov/ij/) | RRID:SCR_003070 | |
| Software | GraphPad Prism software | GraphPad Prism (https://graphpad.com) | RRID:SCR_015807 | Version 8.0.0 |