Fig. 3.

Loss of myelin in the spinal cord in mice at 4 weeks after mild SCI. (A) Klüver-Barrera staining was performed using transverse sections from sham-operated mice, mice at 1 week and 4 weeks after mild SCI. The lesion area in the low magnifications images are surrounded by the dotted lines. The boxes in the low magnification images (top) located in the anterior (middle) and posterior (bottom) spinal regions are magnified. Scale bars, 300, 20 and 20 μm in top, middle and bottom, respectively. Arrows indicate soma of neurons. Sections from 3 mice for each group were stained. (B) Immunofluorescence staining of longitudinal sections of the spinal cord from sham-operated mice and mice at 1 week and 4 weeks after mild SCI with MBP antibody (green). The sections were also stained with DAPI (blue). Sections from 3 mice for each group were stained. Scale bars, 50 μm.