Model-based three-dimensional texture analysis of contrast-enhanced magnetic resonance imaging as a potential tool for preoperative prediction of microvascular invasion in hepatocellular carcinoma

Abstract

The purpose of the present study was to investigate the value of contrast-enhanced magnetic resonance imaging (CE-MRI) texture analysis for preoperatively predicting microvascular invasion (MVI) in hepatocellular carcinoma (HCC). Accordingly, a retrospective study of 142 patients with pathologically confirmed HCC was performed. The patients were divided into two cohorts: The training cohort (n=99) and the validation cohort (n=43), including the MVI-positive group (n=53) and MVI-negative group (n=89). On the basis of three-dimensional texture analysis, 58 features were extracted from the preoperative CE-MR images of arterial-phase (AP) and portal-venous-phase (PP). The t-test or Kruskal-Wallis test, univariate logistic regression analysis and Pearson correlation were applied for feature reduction. Clinical-radiological features were also analyzed. Multivariate logistic regression analysis was used to build the texture model and combined model with clinical-radiological features. The MVI-predictive performance of the models was evaluated using receiver operating characteristic (ROC) analysis and presented using nomogram. Among the clinical features, a significant difference was found in maximum tumor diameter (P=0.002), tumor differentiation (P=0.026) and α-fetoprotein level (P=0.025) between the two groups in the training cohort. Four MR texture features in AP and five in PP images were identified through feature reduction. On ROC analysis, the AP texture model showed better diagnostic performance than did the PP model in the validation cohort, with an area under the curve (AUC) of 0.773 vs. 0.623, sensitivity of 0.750 vs. 0.500, and specificity of 0.815 vs. 0.926. Together with the clinical features, the combined model of AP improved the AUC, sensitivity and specificity to 0.810, 0.811 and 0.790, respectively, which was demonstrated in nomogram. To conclude, model-based texture analysis of CE-MRI could predict MVI in HCC preoperatively and noninvasively, and the AP image shows better predictive efficiency than PP image. The combined model of AP with clinical-radiological features could improve MVI prediction ability.

Introduction

Hepatocellular carcinoma (HCC) is the sixth most common malignant tumor and the second leading cause of tumor mortality worldwide (1,2), and in 2015, its incidence and mortality in China ranked the fourth and third, respectively (3). Surgical resection or liver transplantation has been performed for patients with early stage HCC, and is potentially curative (4). However, the high postoperative recurrence of HCC remains a common problem (5).

In recent years, many clinical studies have shown that microvascular invasion (MVI) is a significant risk factor for the high rate of recurrence and poor prognosis, and could provide information on which to base clinical treatment (6–8). A previous study indicated that the incidence of MVI ranged from 15.0 to 57.1% (8). However, no widely recognized definition of MVI is currently available. The commonly accepted histopathological features of MVI include the presence of tumor cells in the portal veins, in large capsular vessels or in vascular spaces lined by endothelial cells (8,9). As demonstrated in a previous study, a wide resection surgical margin (SM) (58% with SM ≥10 mm vs. 29% with SM <10 mm) may prolong the disease-free survival rate (58 vs. 29%) (10). Unfortunately, MVI can only be detected after postoperative histopathological examination of the whole surgical specimen, and therefore cannot be used for prediction of treatment benefit. If MVI status could be predicted preoperatively and noninvasively, appropriate treatment could be selected to improve the prognosis.

Recently, a number of studies reported that certain clinical features and morphological characteristics detected on imaging examination could predict the MVI status, including the tumor size, tumor margin, capsule formation and dynamic enhancing pattern (10–12). However, clinical and traditional imaging features were more subjective, lacked quantitative indexes and always showed inter-observer differences, thereby leading to poor reliability of the results that were largely affected by the experience level of the radiologist. Functional imaging modalities, including diffusion-weighted imaging, diffusion kurtosis imaging and positron emission tomography-computed tomography (PET-CT), do not have good predictive power because of their instability and lack of reproducibility (13,14). Thus, the current preoperative prediction of MVI based on imaging signs and/or examinations has limitations, mainly the lack of specificity and practicability. Therefore, developing a method to accurately and quantitatively predict the MVI status of HCC preoperatively is of great clinical significance.

Texture analysis is a widely used image post-processing technique that extracts quantitative features from radiological images to explore the correlation between these features and clinical or histological factors (15,16). Texture analysis enables noninvasive assessment of tumor heterogeneity and provides indirect information on the tumor microenvironment that cannot be obtained with the naked eye (17). More recently, texture analysis has been demonstrated to reveal tumor aggressiveness, thus potentially helping predict the risk of disease progression or recurrence and the response to treatment in many studies; this has also enabled its application in CT, magnetic resonance imaging (MRI) and PET-CT studies (18–21). Several studies have also adopted texture analysis for the differential diagnosis of liver nodules, treatment response evaluation of HCC and prognosis prediction (22,23). In addition, model-based texture analysis has been applied to improve diagnostic efficiency, which could assist clinicians in making treatment decisions (24).

In particular, contrast-enhanced MRI (CE-MRI) is generally used for the diagnosis, treatment evaluation, prognosis estimation, as well as MVI prediction of HCC (12). However, no reports to date have documented the use of texture analysis for MVI prediction of HCC, and none have documented the role of CE-MRI texture analysis in predicting MVI.

The purpose of the present study was to explore the value of CE-MRI texture analysis in preoperatively predicting the MVI status of HCC and determining the diagnostic performance to guide the clinician in choosing appropriate treatment options.

Materials and methods

Patient selection

The Independent Ethics Committee of the Cancer Hospital, Chinese Academy of Medical Sciences (Beijing, China) approved the current retrospective study and waived the requirement for informed patient consent. Between January 2014 and December 2016, a total of 282 patients with HCC underwent liver MRI examination confirmed by postoperative pathological examinations. The inclusion criteria were as follows: i) Single tumor with a maximum diameter <5.0 cm, no large vessel invasion and no distant metastasis; ii) underwent radical resection; iii) primary HCC and MVI status confirmed by surgical pathological examination; iv) no other antitumor treatment received before MRI examination and operation; and v) no apparent artifact that may affect imaging analysis. Fig. 1 shows the patient selection flowchart. Consequently, 142 patients were enrolled in the present study and were divided into the MVI-positive (MP) group (n=53) and the MVI-negative (MN) group (n=89). Next, these 142 patients were divided into two cohorts, including the training cohort with 99 patients who underwent MRI examination between January 2014 and January 2016, and the validation cohort with 43 patients who underwent MRI examination between February 2016 and December 2016. The mean time interval between MRI and surgery was 15 days (range, 7–35 days).

Figure 1.

Diagram showing the recruitment of the study population and exclusion criteria. HCC, hepatocellular carcinoma; MRI, magnetic resonance imaging; MVI, microvascular invasion.

Clinicopathological data

The pathological diagnostic criteria for MVI adopted in this study were reported by Rodríguez-Perálvarez et al (8), namely the presence of tumor emboli in a portal vein, hepatic vein or a large capsule vessel, but not a small bile duct of the surrounded hepatic tissue, as shown in Fig. 2A. Clinical and pathological data collected for analysis included sex, age at diagnosis, α-fetoprotein (AFP) level (≤7, 7–400 or >400 ng/ml), tumor location (left, right or caudate lobe), hepatitis B surface antigen (HBsAg) status (positive or negative), hepatitis C antibody (HCV-Ab) and histologic differentiation (well, moderate or poor). The threshold values chosen for AFP were based on the normal ranges and diagnostic value for HCC used at Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College.

Figure 2.

Schematic diagram showing the MVI diagnosis based on pathological examination, prediction using texture analysis and their relationship. (A) Tumor cells could be found in the small vessels near the primary tumor lesion (black arrow). (B) The process of texture analysis includes image segmentation, feature extraction, statistical analysis and model building. MVI, microvascular invasion; GLCM, gray-level co-occurrence matrix; RLM, run-length matrix.

MR image acquisition

The patients fasted for 6–8 h to empty the gastrointestinal tract before undergoing MRI examination. All of the MR images were acquired using a 3.0-T body MRI system (Discovery MR750 3.0T, GE Medical Systems) equipped with an 8-channel phased-array body coil. The CE-MRI acquisitions were performed with multiphase 3D spoiled gradient echo liver acceleration volume acquisition (LAVA) sequence, with the following scanning parameters: Repetition time, 2.9 msec; echo time, 1.3 msec; flip angle, 12°; field of view, 36–42×36–42 cm; matrix, 512×512; section thickness, 4 mm; gap, 0 mm; and number of sections, 36–40. Gadodiamide (Omniscan 0.5 mmol/ml; GE Healthcare) at a standard dose (0.2 ml/kg) was injected as a bolus through the peripheral veins by using an automatic pump injector at the rate of 3.0 ml/sec, and followed immediately by 20 ml of a 0.9% sterile saline solution injection. The contrast-enhanced dynamic images were acquired at 15–20 sec (arterial phase, AP), 50–55 sec (portal venous phase, PP) and 85–90 sec (delayed phase) after contrast-agent injection by using the LAVA sequence.

MRI feature analysis

The basic MRI features included the maximum tumor diameter (MTD) (measured by the maximum diameter on the maximum axial section in PP MR image), liver background (cirrhosis or noncirrhosis), tumor encapsulation (peripheral rim of smooth hyperenhancement in PP image), fast wash-in (hyperenhancement of the tumor in the AP), fast wash-out (hypoenhancement of the tumor in the PP) and tumor necrosis (unenhanced areas). The features for each patient were independently evaluated and recorded in a blinded manner by two radiologists with 5 (YJZ) and 3 years (BF) of experience in the interpretation of abdominal MRI to ensure diagnostic accuracy. When a disagreement occurred between the two reviewers during evaluation, a joint review was performed, and consensus data were used for further statistical analysis.

Tumor imaging segmentation and texture analysis

All the MR images were retrieved from the picture archiving and communication system and transferred to a personal computer in the Digital Imaging and Communications in Medicine format. The same two radiologists reviewed and processed the images in a random patient order by using an in-house developed software, Omni-kinetics (version 2.0.10; GE Healthcare Life Sciences), to obtain texture features. A 3D volume of interest (VOI) of the tumor was manually contoured by the two readers, slightly along the borders of the tumor to include the entire approximated tumor volume.

After generating the VOI, a total of 58 texture features were automatically extracted from the AP and PP images using the Omni-kinetics software. The texture features could be divided into four categories: i) 29 histogram features, ii) 8 gray-level co-occurrence matrix (GLCM) features, iii) 11 Haralick features, and iv) 10 run-length matrix (RLM) features. A detailed list of the features included in the present study is presented in Table I. Fig. 2B shows the diagram of texture analysis.

Table I.

List of 58 texture analysis parameters.

Texture type	Texture parameters
Histogram	MinIntensity, MaxIntensity, MedianIntensity, MeanValue, stdDeviation, Variance, VolumeCount, VoxelValueSum, RMS, Range, MeanDeviation, RelativeDeviation, MinLocation, MaxLocation, Skewness, kurtosis, uniformity, Energy, Entropy, FrequencySize, MPP, UPP, Quantile5, Quantile10, Quantile25, Quantile50, Quantile75, Quantile90, Quantile95
GLCM	GlcmTotalFrequency, GlcmEnergy, GlcmEntropy, Inertia, Correlation, InverseDifferenceMoment, ClusterShade, ClusterProminence
Haralick	HaralickCorrelation, HaraEntropy, AngularSecondMoment, contrast, HaraVariance, sumAverage, sumVariance, sumEntropy, differenceVariance, differenceEntropy, inverseDifferenceMoment
RLM	ShortRunEmphasis, LongRunEmphasis, GreyLevelNonuniformity, RunLengthNonuniformity, LowGreyLevelRunEmphasis, HighGreyLevelRunEmphasis, ShortRunLowGreyLevelEmphasis, ShortRunHighGreyLevelEmphasis, LongRunLowGreyLevelEmphasis, LongRunHighGreyLevelEmphasis

RMS, Root mean square; GLCM, gray-level co-occurrence matrix; MPP, mean value of positive pixels; UPP, uniformity of distribution of positive gray-level pixel values; RLM, run-length matrix.

Statistical analysis and feature reduction

The Kolmogorov-Smirnov test was used to determine whether the distribution of all the features was normal, and Levene's test was used for identifying the homogeneity of variance. For the clinical-radiological features, a two-tailed unpaired independent t-test was used to compare continuous variables with normal distribution between the MP and MN groups. Categorical variables were compared using the χ² test or Fisher's exact test.

For texture features, first, an independent t-test or Kruskal-Wallis test was applied one by one. Features with significant differences (P<0.05) were further analyzed by univariate logistic regression analysis. Features in the univariate logistic regression analysis with P<0.05 were selected. Finally, to eliminate redundant features, Pearson correlation analysis was conducted to remove features with high correlation (r>0.90), which were not considered in the subsequent analysis. Features that remained after adjusting for redundancy were entered into model building.

All statistical analyses were performed using R software (version 3.4.1; R Foundation for Statistical Computing), with a two-tailed probability value. P<0.05 was considered to indicate a statistically significant difference.

Model development

Multivariate logistic regression analysis was applied for model building. First, the texture model was built on the basis of the features selected from a previous step by directly entering. The texture signature score (Texscore), which reflected the overall texture features, was calculated for each patient using the texture model. To involve both texture features and clinical-radiological features to improve performance, the combined model was built on the basis of the Texscore as well as other significant clinical-radiological features in both AP and PP.

Receiver operating characteristic (ROC) analysis and the nomogram plot of the model

The performance of the texture and combined models was analyzed both in the training and validation cohorts by using a ROC curve quantified by the area under the curve (AUC), sensitivity, specificity and overall accuracy (ACC). The cutoff point was calculated at the maximized value of the Youden index (sensitivity + specificity-1) (25). Nomogram of the combined model was formulated in order to visualize model efficiency.

Results

Clinical and radiological features

Of the 142 patients enrolled in the study, 124 patients were male and 18 were female. Median age was 57 years (range, 34–80 years). The results revealed that MTD (P=0.002), serum AFP level (P=0.025) and tumor differentiation (P=0.026) showed significant differences between the MP and MN groups in the training cohort. The MP group had greater MTD than did the MN group (3.82±0.88 vs. 3.21±0.94 cm), and also tended to have a higher serum AFP level and lower tumor differentiation. This result was also confirmed in the validation cohort. The characteristics of the training and validation cohorts showed no significant differences (all P>0.05). The detailed clinical and radiological features of the patients in the training and validation cohorts are listed in Table II.

Table II.

The clinical and radiological characteristics of patients in the training and validation cohorts.

A, Clinical characteristics
	Training cohort (n=99)			Validation cohort (n=43)
Characteristic	MP (n=37)	MN (n=62)	P-valuea	MP (n=16)	MN (n=27)	P-valuea	P-valueb
Sex			0.931c			0.723c	0.805c
Male	32	54		15	23
Female	5	8		1	4
Mean age ± SD, years	57.49±9.56	56.45±9.71	0.607e	55.88±8.63	55.41±8.00	0.858e	0.456e
AFP, ng/ml			0.025c			0.038c	0.715c
≤7	9	32		5	16
7–400	17	20		5	9
>400	11	10		6	2
Location			0.561c			0.372c	0.581c
Right	31	49		10	23
Left	6	13		6	4
HBsAg			0.325c			0.614c	0.668c
Positive	26	49		12	22
Negative	11	13		4	5
HCV-Ab			1.000c			1.000d	0.989c
Positive	1	3		0	1
Negative	36	59		16	26
Differentiation			0.026c			0.034d	0.565c
Well	2	3		0	4
Moderate	19	47		9	20
Poor	16	12		7	3
B, Radiological characteristics
	Training cohort (n=99)			Validation cohort (n=43)
Characteristic	MP (n=37)	MN (n=62)	P-valuea	MP (n=16)	MN (n=27)	P-valuea	P-valueb
MTD, cm	3.82±0.88	3.21±0.94	0.002e	3.75±0.80	2.92±0.62	<0.001e	0.231e
Background liver			0.690c			0.358c	0.553c
Noncirrhosis	14	21		3	10
Cirrhosis	23	41		13	17
Tumor encapsulation			0.805c			0.362c	0.534c
Absent	11	17		2	8
Present	26	45		14	19
Fast wash-in			0.292c			0.929c	0.742c
Yes	30	55		14	22
No	7	7		2	5
Fast wash-out			0.409c			0.534c	0.720c
Yes	24	35		11	16
No	13	27		5	11
Tumor necrosis			0.638c			0.372c	0.668c
Absent	29	46		11	23
Present	8	16		5	4

MP, microvascular invasion-positive; MN, microvascular invasion-negative; AFP, α-fetoprotein; HBsAg, hepatitis B surface antigen; MTD, maximum tumor diameter; HCV-Ab, hepatitis C antibody.

^aMP vs. MN

^bTraining cohort vs. validation cohort

^cP-values calculated using the χ² test

^dP-values calculated using Fisher's exact test

^eP-values calculated using the independent t-test.

Texture feature reduction

First, the Kruskal-Wallis test revealed that 19 texture features in AP and 18 in PP showed significant differences (all P<0.05) between the MP and MN groups. In the univariate logistic regression analysis, 10 texture features in AP and 12 in PP showed a potential predictive value (P<0.05) to discriminate between the MP and MN groups. Detailed results of the univariate logistic regression analysis are demonstrated in Table III. Finally, a Pearson correlation matrix was then calculated from the above features to detect highly collinear texture features (Fig. 3). After eliminating significant highly correlated features (defined as Pearson's r≥0.90), four texture features in AP, including uniformity, ClusterProminence, ClusterShade and LongRunLowGreyLevelEmphasis (LRLGLE), and five in PP, including MinIntensity, GlcmEntropy, sumAverage, sumEntropy and RunLengthNonuniformity (RLN), were retained for the subsequent analysis.

Table III.

Univariate logistic regression analysis of the clinical and texture features to predict the microvascular invasion status in the training cohort.

	OR
		95% CI
Feature	Value	Lower	Upper	P-value
AP texture features
Uniformity	1.209×104	6.602	4.605×107	0.019
Energy	3.110×10121	4.556×1019	1.820×10229	0.022
Entropy	1.123×10−1	1.991×10−2	5.647×10−1	0.010
UPP	3.110×10121	4.556×1019	1.820×10229	0.022
ClusterShade	1.000	1.000	1.000	0.046
ClusterProminence	1.000	1.000	1.000	0.005
GreyLevelNonuniformity	1.003	1.001	1.007	0.023
LowGreyLevelRunEmphasis	0.000	0.000	3.320 ×10−192	0.034
ShortRunLowGreyLevelEmphasis	0.000	0.000	2.140×10−255	0.035
LongRunLowGreyLevelEmphasis	0.000	0.000	2.060 ×10−79	0.036
PP texture features
MinIntensity	9.973×10−1	9.947×10−1	9.995×10−1	0.025
VolumeCount	1.000	1.000	1.000	0.027
Uniformity	1.087×10−3	9.070×10−7	6.241×10−1	0.044
FrequencySize	1.000	1.000	1.000	0.027
GlcmTotalFrequency	1.000	1.000	1.000	0.026
GlcmEntropy	6.627×10−1	4.454×10−1	9.462×10−1	0.030
HaraEntroy	3.266×105	2.273×101	1.286×1010	0.013
AngularSecondMoment	0.000	0.000	4.329×10−94	0.026
sumAverage	1.301×10−2	2.238×10−4	5.748×10−1	0.029
sumEntropy	1.896×104	4.127	1.996×108	0.028
GreyLevelNonuniformity	1.003	1.001	1.006	0.024
RunLengthNonuniformity	1.000	1.000	1.000	0.025

OR, odds ratio; CI, confidence interval; UPP, uniformity of distribution of positive gray-level pixel values; AP, arterial phase; PP, portal venous phase.

Figure 3.

Pearson correlation matrix plot for (A) 10 texture features in the arterial phase and (B) 12 texture features in the portal venous phase of the training cohort. Blue circles indicate positive correlation, red circles negative correlation. The larger the circle and the darker the color, the higher is the correlation between two texture features. GLN, GreyLevelNonuniformity; LRLGLE, LongRunLowGreyLevelEmphasis; LGLRE, LowGreyLevelRunEmphasis; SRLGLE, ShortRunLowGreyLevelEmphasis; RLN, RunLengthNonuniformity; ASM, AngularSecondMoment; UPP, uniformity of distribution of positive gray-level pixel values.

Multivariate logistic regression analysis and texture model building

Texture features identified from the above analysis in AP and PP were entered into the multivariate logistic regression model to build the texture predictive model. The detailed results of the multivariate logistic regression analysis are shown in Table IV.

Table IV.

Multivariate logistic regression analysis of the texture parameters in predicting the microvascular invasion status in the arterial and portal venous phases in the training cohort.

A, Arterial phase
Feature	OR	P-value
(Intercept)a	0.455	0.003
Uniformity	1.301	0.387
ClusterShade	0.524	0.059
ClusterProminence	0.593	0.262
LRLGLE	0.494	0.170
B, Portal venous phase
Feature	OR	P-value
(Intercept)a	7.310	0.754
GlcmEntropy	0.732	0.146
sumEntropy	0.002	0.474
sumAverage	2.230e+05	0.124
MinIntensity	0.999	0.563
RLN	1.000	0.371

^aIntercept is the constant term in the logistic regression equation. OR, odds ratio; LRLGLE, LongRunLowGreyLevelEmphasis; RLN, RunLengthNonuniformity.

The texture signature score (Texscore) of each patient in AP and PP could be calculated using the formula based on multivariate logistic regression model as follows: Texscore (AP) = 0.455+1.30 × uniformity + 0.524 × ClusterProminence + 0.593 × ClusterShade + 0.494 × LRLGLE; and Texscore (PP) = 7.310 + 0.732 × GlcmEntropy + 0.002 × sumEntropy + 2.230×10⁵ × sumAverage + 0.999 × MinIntensity + 1.000 × RLN.

Development of the combined model

In the training cohort, two combined models were built to predict the MVI status by clinical-radiological features and the Texscore generated above in AP and PP separately (Table V). In the combined model in AP, only the texture signature showed significant [odds ratio (OR), 2.552; P=0.003], but none of the clinical-radiological features showed significance. In the combined model in PP, MTD (OR, 0.759; P=0.032) and AFP (OR, 1.307; P=0.027 and OR, 1.768; P=0.017) showed significance, but the texture signature and differentiation did not show significance.

Table V.

Multivariate logistic regression analysis of the combined clinical-radiological and texture features to predict the microvascular invasion status in the AP and PP in the training cohort.

	AP Model		PP Model
Features	OR	P-value	OR	P-value
(Intercept)a	0.257	0.282	−3.068	0.052
MTD, cm	1.395	0.242	0.759	0.032
Differentiation
Moderate vs. poor	0.559	0.284	−0.838	0.179
Well vs. low	0.672	0.747	−0.697	0.560
α-fetoprotein, ng/ml
7–400 vs. ≤7	1.801	0.298	1.307	0.027
>400 vs. ≤7	3.771	0.053	1.768	0.017
Texscore	2.552	0.003	0.449	0.294

^aIntercept is the constant term in the logistic regression equation, and is not a clinical-radiological feature. AP, arterial phase; PP, portal venous phase; OR, odds ratio; MTD, maximum tumor diameter.

Nomograms in AP and PP

The nomograms integrating the features included in the multivariate logistic regression analysis are displayed in Fig. 4, which could visualize the weight of different features in combined model. The concordance indexes of the nomograms for MVI predictions in AP and PP were 0.810 [95% confidence interval (CI), 0.718–0.902] and 0.799 (95% CI, 0.710–0.889), respectively.

Figure 4.

Nomograms for predicting the microvascular invasion status of hepatocellular carcinoma in (A) the arterial phase and (B) portal venous phase using the texture signature and clinical-radiological features. AFP) levels 1, 2 and 3 stand for ≤7, 7–400 and >400 ng/ml, respectively. Differentiation levels 1, 2 and 3 stand for poor, moderate and well differentiation, respectively. MTD, maximum tumor diameter; AFP, α-fetoprotein.

Predictive performance of MVI

For the training cohort, the ROC curves illustrating the predictive performance of the texture and combined models in predicting the MVI status are provided in Fig. 5. The AUC of the texture model was 0.765 vs. 0.707 in AP and PP, respectively. The AUC of the combined model was 0.810 vs. 0.799 in AP and PP, respectively. The AUC, ACC, sensitivity and specificity for each model are summarized in Table VI.

Figure 5.

ROC curves for the texture and combined models in predicting the microvascular invasion status in the training cohort. The solid dots represent the optimal cutoff values (specificity, sensitivity) for discrimination. The AUC for the texture models in (A) AP and (B) PP are 0.765 and 0.707, respectively. The AUC for the combined models in (C) AP and (D) PP are 0.810 and 0.799, respectively. ROC, receiver operating characteristic; AUC, area under the curve; AP, arterial phase; PP, portal-venous phase.

Table VI.

Predictive performance of the texture and combined models in predicting the microvascular invasion status in the training and validation cohorts.

		Training cohort (n=99)				Validation cohort (n=43)
Model	Phase	AUC	ACC	SEN	SPE	AUC	ACC	SEN	SPE
Texture	AP	0.765	0.768	0.730	0.790	0.773	0.791	0.750	0.815
	PP	0.707	0.727	0.622	0.790	0.623	0.767	0.500	0.926
Combined	AP	0.810	0.798	0.811	0.790	0.794	0.837	0.812	0.852
	PP	0.799	0.758	0.730	0.774	0.706	0.721	0.750	0.704

AP, arterial phase; PP, portal venous phase; AUC, area under the curve; ACC, overall accuracy; SEN, sensitivity; SPE, specificity.

The validation cohort was used to verify the accuracy of the model built in the training cohort. The ROC curves of the texture and combined models for predicting the MVI status are shown in Fig. 6. The AUC of the texture model was 0.773 vs. 0.623 in AP and PP, respectively. The AUC of the combined model was 0.794 vs. 0.706 in AP and PP, respectively. The AUC, ACC, sensitivity and specificity for each model are summarized in Table VI.

Figure 6.

ROC curves for the texture and combined models in predicting the microvascular invasion status in the validation cohort. The solid dots represent the optimal cutoff values (specificity, sensitivity) for discrimination. The AUC for the texture models in the (A) AP and (B) PP are 0.773 and 0.623, respectively. The AUC for the combined models in (C) AP and (D) PP are 0.794 and 0.706, respectively. ROC, receiver operating characteristic; AUC, area under the curve; AP, arterial phase; PP, portal-venous phase.

Discussion

In the present study, a combined model was developed based on preoperative 3D CE-MRI texture and clinical-radiological features to predict MVI with a satisfactory discriminatory performance. The results indicated that texture analysis is a potentially useful adjunct for predicting MVI, and adding clinical-radiological data could slightly improve the predictive ability. In addition, the AP texture features performed better than the PP texture features in MVI prediction.

The present study involved a total of 142 patients, 53 of which were MVI-positive patients and 89 MVI-negative. The MVI-positive rate was consistent with the reported rate in the literature (8), which was approximately 30.0%. Previous studies have reported that the tumor size of HCC was one of the most important predictive factors for MVI (26,27). This is consistent with the results of the current study. In the training cohort, MTD in the MP group (3.82±0.88 cm) was significantly greater than that in the MN group (3.21±0.94 cm) (P=0.002), since tumor size is an important feature of tumor burden. Moreover, patients with poorer tumor histological differentiation were observed in the MP group (P=0.026), and this feature has been reported to be a predictor of MVI in previous studies (28,29), possibly due to the high invasiveness of poorly differentiated tumors. In the present study, the result showed that the MP group tended to have higher AFP levels (P=0.025). Serum AFP level is considered a marker of HCC, which has also been reported to correlate with MVI (30). Nevertheless, the mechanism needs to be further clarified. In the validation cohort, the above results were confirmed again.

CE-MRI texture analysis of the AP and PP images was used to build models for predicting the MVI status, and the AP images showed better predictive ability than did the PP images. Four texture features in AP (uniformity, ClusterProminence, ClusterShade and LRLGLE) and five in PP (MinIntensity, GlcmEntropy, sumAverage, sumEntropy and RLN) were entered into the multivariate logistic regression analysis. These features frequently appeared in texture or radiomics research and showed noteworthy diagnostic and predictive efficiency, which could be explained by their definitions (31,32). All these features reflect the heterogeneity in an image from a different aspect, and were thus related to tumor heterogeneity from a clinical point of view. Previous studies have shown that tumor heterogeneity is related to tumor differentiation, angiogenesis and prognosis (33,34). On the basis of the current results, tumor heterogeneity may be associated with the occurrence of MVI.

In the multivariate logistic regression analysis, the directly enter mode was used to build the predictive model, but the P-values of the texture features in the texture model were not significant. The possible explanation was that all parameters were equally important, and hence, no significant texture features were observed in the regression. The combined model was also built by adding the clinical-radiological features to the multivariate logistic regression. The nomogram was used to visualize the combined model and to reveal the weight of the clinical-radiological and texture features. For clinical use of the model, the total scores of each patient could be calculated based on the nomogram. High scores corresponded to a high probability of MVI occurrence. The nomogram showed that the texture signature accounted for a higher proportion in the total points than did the clinical-radiological features in AP.

In the ROC analysis, the combined model showed a little better predictive performance than did the texture model in the validation cohort both in AP (AUC, 0.794 vs. 0.773; specificity, 0.852 vs. 0.815; sensitivity, 0.812 vs. 0.750) and in PP (AUC, 0.706 vs. 0.623; specificity, 0.704 vs. 0.926; sensitivity, 0.750 vs. 0.500). The predictive ability was better in AP than in PP. The possible explanations might be that the blood supply to the HCC is mainly dependent on the hepatic artery, and that the AP image could more clearly reflect the changes in small blood vessels in the HCC. Moreover, when MVI occurs, the local hemodynamics of the liver tissue around the tumor changes. Therefore, the changes in MRI texture features in AP were more obvious than those in PP.

From a clinical perspective, the results of the present study suggest that CE-MRI texture analysis may be an option for preoperatively predicting MVI in HCC. This could alert pathologists to conduct more detailed pathological examinations, particularly when preoperative texture analysis suggests a high possibility of MVI occurrence. Meanwhile, predicting the possibility of MVI occurrence could also help clinicians select more suitable surgical procedures for HCC patients. A number of studies have shown that anatomic resection (35,36) and wide resection SM (10) may provide radical treatment in HCC patients with MVI. Intrahepatic metastases resulting from MVI could also be reduced, thereby improving the prognosis.

The present study had the following advantages. First, to our knowledge, this was the first study to use 3D MRI texture features to build a model for predicting the MVI status preoperatively and noninvasively. The 3D VOI may have a better predictive performance since MVI could occur in every slice, and 3D feature analysis considers all of the available slices with abundant information. Previous studies have also shown that 3D features improved the diagnostic accuracy than did two-dimensional features (37,38). Second, both the AP and PP images were analyzed to compare their predictive performance. Third, training and validation cohorts were used, making the model more objective. Therefore, the process and method established in the present study provide a basis for further computer-aided and artificial-intelligence analyses for predicting MVI in HCC.

The current study also had several limitations. First, this was a retrospective study with a single-center design; therefore, selection bias was unavoidable. Second, the sample size was small for texture analysis. More cases are needed to verify the results. Third, the manual segmentation of tumors may have introduced a certain amount of subjectivity. The MVI grade in the positive group was not taken into account. Forth, the present study did not include des-γ-carboxy-prothrombin (DCP) as it was not tested routinely in our hospital, although a study has reported that DCP was useful for prediction of MVI (39). At last, no follow-up and survival analysis were performed, and hence, the relationship between prognosis and texture features could not be further clarified.

In conclusion, model-based texture analysis of CE-MRI could predict MVI in HCC preoperatively and noninvasively. The AP image shows better predictive efficiency than PP image. The combined model of AP with clinical-radiological features could improve MVI prediction ability. It may be of value to clinicians in objectively selecting appropriate treatment strategies and as an individualized predictive tool for improving clinical outcomes.

Glossary

Abbreviations

CE-MRI

contrast-enhanced magnetic resonance imaging

MVI

microvascular invasion

HCC

hepatocellular carcinoma

3D

three-dimensional

AP

arterial phase

PP

portal-venous phase

ROC

receiver operating characteristic

AUC

area under the curve

SM

surgical margin

PET-CT

positron emission tomography-computed tomography

MP

MVI-positive

MN

MVI-negative

AFP

α-fetoprotein

LAVA

liver acceleration volume acquisition

MTD

maximum tumor diameter

VOI

volume of interest

GLCM

gray-level co-occurrence matrix

Texscore

texture signature score

ACC

overall accuracy

LRLGLE

LongRunLowGreyLevelEmphasis

RLN

RunLengthNonuniformity

CI

confidence interval

Funding

This study was supported by the Peking Union Medical College Youth Fund and the Fundamental Research Funds for the Central Universities (grant no. 2017320010), the Chinese Academy of Medical Sciences (CAMS) Research Fund (grant no. ZZ2016B01) and the CAMS Innovation Fund for Medical Sciences (grant no. 2016-I2M-1-001).

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Authors' contributions

YJZ, SW, LMW, XHM and XMZ designed this study. YJZ, BF and LMW acquired the patients and searched the database. YJZ and BF participated in the process of texture analysis, including segmentation and features extraction. JFW contributed to the analysis and interpretation of data, including statistical analysis, biostatistics and computational analysis. YJZ and XHM were major contributors in writing the manuscript. All authors read and approved the final manuscript.

Ethics approval and consent to participate

The independent ethics committee of the Cancer Hospital, Chinese Academy of Medical Sciences approved this retrospective study and waived the requirement for informed patient consent.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.