Hu Q., Zhu L., Zhang X., Guan Q., Xiao S., Min L., and Zhang X. GhCPK33 Negatively Regulates Defense against Verticillium dahliae by Phosphorylating GhOPR3.
An error in the bioinformation analysis in prediction of peroxisomal targeting signal (PTS) of GhCPK33 (PTS1 and PTS2, the PTS1 always localized at very C-terminal, and PST2 always localized near the N terminus or at internal locations in proteins), resulted in GhCPK33 having no typical PTS1 sequence. The authors reanalyzed the amino acid sequence of GhCPK33 and found a typical PTS2 sequence according to Flynn et al. (1998; Plant journal). There was no typical PTS1 sequence found. This affects text in the Results section of the article, which has been revised to state the following:
We cloned the full-length cDNA of GhCPK33 from cotton cv YZ1, which consists of 1614 nucleotides and encodes a protein of 537 amino acids with an N-terminal myristoylation sequence and palmitoylation site, and a predicted peroxisomal targeting signal 2 according to Flynn et al. (1998; Supplemental Fig. S1).
The legend of Supplemental Figure S1 has also been revised as follows:
Supplemental Figure S1. Characterization of GhCPK33. Predicted conserved domains were determined by an online NCBI CDD program. GhCPK33 is a member of Ca2+/Calmodulin-dependent protein kinase family (CAMK), with typical Ca2+ binding EF hand and N-terminal myristoylation sequence and palmitoylation site, and peroxisomal targeting signal 2.
The original results and conclusions of the article are unaffected by these revisions.