Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background | AAV(9)-CMV-dFRT-hM3Dq-mCherry | This paper | NA | Titer: 1.0 × 1012particles/ml |
| Strain, strain background | AAV(DJ)-CMV-dFRT-hrGFP | This paper | NA | Titer: 3.7 × 1012particles/ml |
| Strain, strain background | AAV(DJ)-CMV-FLEX-tdTomato | This paper | NA | Titer: 2.0 × 1012particles/ml |
| Genetic reagent (Mus musculus) | Orexin-Flippase | This paper | Orexin-Flp | Knock-in mice; EGFP-2A-Flp was placed just behind the translational initiation site of the hypocretin gene in-frame |
| Genetic reagent (Mus musculus) | Glutamic acid decarboxylase 67-Cre | Higo et al., 2009 | Gad67-Cre | |
| Genetic reagent (Mus musculus) | R26-CAG-FRT-STOP-FRT-mTFP1 | Imayoshi et al., 2012 | FSF-mTFP1 | |
| Genetic reagent (Mus musculus) | Melanin-concentrating hormone-Cre | Kong et al., 2010 | MCH-Cre | |
| Genetic reagent (Mus musculus) | Orexin-enhanced green fluorescence protein | Yamanaka et al., 2003 | Orexin-EGFP | |
| Antibody | goat polyclonal anti-orexin | Santa Cruz Biotechnology | sc-8070 | (1/1000) |
| Antibody | rabbit polyclonal anti-MCH | Sigma-Aldrich | M8440 | (1/2000) |
| Antibody | rabbit polyclonal anti-prodynorphin | Merck Millipore | AB5519 | (1/100) |
| Antibody | mouse monoclonal anti-c-Fos | Santa Cruz Biotechnology | E-8 | (1/500) |
| Antibody | mouse monoclonal anti-DsRED | Santa Cruz Biotechnology | sc-390909 | (1/1000) |
| Antibody | mouse monoclonal anti-GFP | Fujifilm Wako Pure Chemical Corporation | mFX75 | (1/1000) |
| Sequence-based reagent (primer) | forward primer: 5’-CTCATTAGTACTCGGAAACTGCCC-3’ | This paper | NA | Primer for PCR genotyping of orexin-Flp mouse tail DNA |
| Sequence-based reagent (primer) | reverse primer: 5’-AAGCACTATCATGGCCTCAGTAGT-3’ | This paper | NA | Primer for PCR genotyping of orexin-Flp mouse tail DNA |
| Chemical compound, drug | clozapine-N-oxide (CNO) | Enzo Life Sciences | BML-NS105-0025 | |
| Chemical compound, drug | Picrotoxin | Sigma-Aldrich | P1675 | |
| Chemical compound, drug | tetrodotoxin (TTX) | Alomone Labs | Cat# T-550 | |
| Chemical compound, drug | D-2-Amino-5-phosphopentanoic acid (AP5) | Alomone Labs | Cat# D-145 | |
| Chemical compound, drug | 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) | Sigma-Aldrich | Cat# C127 | |
| Chemical compound, drug | Lidocaine N-ethyl bromide (QX-314 bromide) | Alomone Labs | Cat# Q-100 | |
| Software, algorithm | Origin 2017 | Lightstone | Origin 2018 | |
| Software, algorithm | SleepSign | Kissei Comtec | Version 3 | |
| Software, algorithm | pClamp 10.5Software and Algorithms | Molecular Devices | RRID:SCR_011323 | |
| Software, algorithm | ImageJ | https://imagej.nih.gov/ij/ | RRID:SCR_003070 | |
| Other | DAPI stain | Thermo Fisher Scientific | Cat# D1306 |