Table 1:

Metabolic activity of fresh, cryopreserved and bulk droplet vitrified hepatocytes in long-term collagen sandwich cultures.

		Fresh	Cryo	Vitrified	P values
		Mean ± SD	Mean ± SD	Mean ± SD	Fresh-Cryo	Fresh-Vitrified	Cryo-Vitrified
Presto Blue	Day 3	1152 ± 241	565 ± 213	643 ± 271	<0.001	<0.001	0.660
Units: RFU	Day 5	787 ± 200	541 ± 319	696 ± 339	0.033	0.569	0.214
	Day 7	647 ± 76	255 ± 46	341 ± 107	0.001	0.008	0.606
Urea production	Day 2	7.2 ± 1.2	3.6 ± 1.5	4.2 ± 1.4	<0.001	<0.001	0.693
Units: μg/1e6 hepatocytes/24hr	Day 3	7.1 ± 1.3	3.1 ± 1.1	4.5 ± 1.1	<0.001	0.001	0.122
	Day 4	5.5 ± 2.6	3.6 ± 1.0	4.5 ± 1.3	0.007	0.271	0.232
	Day 5	5.4 ± 2.4	3.0 ± 1.8	6.0 ± 1.4	0.002	0.665	<0.001
	Day 6	5.8 ± 3.1	2.3 ± 0.8	4.8 ± 2.2	<0.001	0.326	0.002
	Day 7	6.8 ± 2.5	2.9 ± 2.0	4.9 ± 1.8	<0.001	0.019	0.013
Albumin synthesis	Day 2	0.65 ± 0.30	0.27 ± 0.14	0.45 ± 0.18	<0.001	0.019	0.045
Units: μg/1e6 hepatocytes/24hr	Day 3	0.74 ± 0.32	0.24 ± 0.13	0.46 ± 0.01	<0.001	0.001	0.011
	Day 4	0.75 ± 0.33	0.28 ± 0.12	0.51 ± 0.12	<0.001	0.006	0.008
	Day 5	0.75 ± 0.36	0.31 ± 0.19	0.64 ± 0.07	<0.001	0.284	<0.001
	Day 6	0.84 ± 0.31	0.27 ± 0.22	0.62 ± 0.29	<0.001	0.011	<0.001
	Day 7	1.11 ± 0.19	0.36 ± 0.25	0.69 ± 0.21	<0.001	<0.001	<0.001

Note. Cryo = cryopreserved; Vitrified = bulk droplet vitrified; RFU = relative fluorescence units.