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. 2019 May 3;8:e44760. doi: 10.7554/eLife.44760

Table 3. Kinetic activity parameters for point-mutated PC-binding-site residues in the C2-domain of cPLA2α*.

Protein KsA (μM) Vmax (nmol/min/mg)
WT-cPLA2α 182.8 ± 12.5 4.053 ± 0.092
Y96F-cPLA2α 205.8 ± 12.7 4.930 ± 0.111
Y96A-cPLA2α 467.5 ± 31.6 3.438 ± 0.177
N65D-cPLA2α 394.9 ± 29.8 3.203 ± 0.166
N64A-cPLA2α 207.7 ± 9.63 4.328 ± 0.069

*Analyses for data shown in Figure 3E.