Early repair intermediates accumulate to a greater extent in atm-1 mutants. (A) Schematic of the C. elegans germ line showing regions in which RAD-51 foci were quantified. Mit: Mitotic zone. TZ: transition zone. (B, C, E, F, H, and I) The percentage of nuclei in each zone containing the indicated number of RAD-51 foci shown in the color key at the bottom. Numbers represent total number of nuclei counted/zone for three gonads/genotype. (D, G, and J) Comparison of the average number of RAD-51 foci for each genotype. # = nuclei scored/3 germ lines/genotype. χsquare, atm-1
vs. N2; atm-1;him-5
vs.
him-5; and atm-1;dsb-2
vs.
dsb-2: P < 0.0001 for each. Representative images of germ lines are shown in Figure S1.