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. 2018 Dec 13;57(3):466–474. doi: 10.1007/s10528-018-9899-9

Screening of Genetic Factor in the Interaction Between Periodontitis and Metabolic Traits Using Candidate Gene Association Study (CGAS)

Kyung-Hui Moon 1,✉
PMCID: PMC6556154  PMID: 30547318

Abstract

Periodontitis has been reported to relate to metabolic syndrome traits such as obesity, blood pressure, and so on. However, the relation between periodontitis and metabolic syndrome remains unclear. The present study aimed to confirm common genetic factors between periodontitis and metabolic traits using Candidate gene association study (CGAS) in the Korean population. Based on the analysis of CGAS, this study performed linear regression analyses to examine the single-nucleotide polymorphisms (SNPs) between periodontitis and metabolic syndrome traits. Among the analyzed SNPs, 2649 SNPs in five genes (TENM2, LDLRAD4, SLC9C2, MFSD1, and A2BP1) showed a statistical significance at p < 0.05. Interestingly, A2BP1 and TENM2 were related to obesity. Also, elevated levels of LDLRAD4, SLC9C2, and MFSD1 were observed in the patients with high blood pressure. Taken together, the present study suggests that some of the SNPs are related to periodontitis. Therefore, if any of TENM2, A2BP1, LDLRAD4, SLC9C2, and MFSD1 is detected in the patients with periodontitis, obesity and blood pressure have to be treated simultaneously.

Electronic supplementary material

The online version of this article (10.1007/s10528-018-9899-9) contains supplementary material, which is available to authorized users.

Keywords: Korean, Periodontitis, A2BP1, TENM2, LDLRAD4, Metabolic traits

Introduction

Periodontitis is a chronic inflammatory disease by bacterial infection of the tissues supporting the teeth (Haffajee and Socransky 1994; Page and Kornman 1997). It was reported that the development of periodontitis is an irreversible process (Nelson-Filho et al. 2018). Unfortunately, it is a major cause of tooth loss in adults. Periodontitis in the Korean population accounted for amount to 10.2% to 55.7% depending on age (Kim et al. 2014).

Interestingly, major cause of periodontitis was various diseases, such as dyslipidemia, glucose intolerance, hypertension, and a low-grade systemic inflammatory state (Winning and Linden 2017; Lamster and Pagan 2017), as well as with systemic diseases and conditions termed metabolic traits such as cardiovascular disease, diabetes, and obesity (Hong et al. 2015). Furthermore, a number of studies have shown that periodontitis resulted from periodontal microorganisms and smoking (Socransky et al. 1998; Socransky and Haffajee 2005; Gelskey 1999). Recent a study using cross-sectional and longitudinal designs demonstrated an association between periodontitis and metabolic traits (Nibali et al. 2013). However, there are contradictory reports showing no association between periodontitis and metabolic traits (Nibali et al. 2013). This might be due to age, gender, socioeconomic status genetic factor, and lifestyle (Genco and Borgnakke 2013). Nevertheless, the genes inducing periodontitis have already been known as genetic risk markers of multifactorial diseases (Sanders et al. 2017; Hong et al. 2015).

Interestingly, a genetic study on CGAS of the Korean periodontitis has focused upon ten periodontitis genes (TENM2, LDLRAD4, SLC9C2, RASGRP4, MFSD1, IL4, NMUR2, GPR141, GLK, and A2BP1) identified as the potential candidate genes with genetic risk factors (see Table 1) (Hong et al. 2015).

Table 1.

Reported genetic risk factors in Korean Periodontitis

SNP Gene Effect of size p value Ref 1 Ref 2 Ref 3
rs4242220 TENM2 0.53 2.84 × 10−6 Hong et al. (2015)
rs12969041 LDLRAD4 2.86 2.79 × 10−7 Hong et al. (2015)
rs2027756 LDLRAD4 2.86 2.79 × 10−6 Hong et al. (2015)
rs16846206 SLC9C2 2.02 7.66 × 10−5 Hong et al. (2015)
rs892055 RASGRP4 0.49 1.23 × 10−4 Hong et al. (2015)
rs1346834 MFSD1 0.71 0.007 Hong et al. (2015) Teumer et al. (2013)
rs2243250 IL4 0.65 0.004 Hong et al. (2015) Laine et al. (2012) Divaris et al. (2013)
rs2070874 IL4 0.66 0.006 Hong et al. (2015) Laine et al. (2012) Divaris et al. (2013)
rs294958 NMUR2 1.29 0.034 Hong et al. (2015) Teumer et al. (2013)
rs2392510 GPR141 1.48 9.48 × 10−4 Hong et al. (2015) Shimizu et al. (2015)
rs2243407 BLK 0.73 0.01 Hong et al. (2015) Teumer et al. (2013)
rs11866781 A2BP1 1.32 0.045 Hong et al. (2015) Teumer et al. (2013)
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Because periodontitis triggered metabolic syndrome traits, the roles of these genes in periodontitis should be confirmed. Nevertheless, the functions of these genes have not been clearly understood so far.

To clearly verify the relation between periodontitis and metabolic traits, single-nucleotide polymorphisms (SNPs) were investigated after the screening of the candidate genes. However, considering that gene was repetitively replicated, the SNP study did not provide convincing evidence for the presence of risk alleles.

Candidate gene association study (CGAS) is a bias-free approach for the identification of risk genes. Recently, a study analyzed four genetic associations in chronic European and Japanese populations (Giacomini et al. 2017), and then the most harmful risk alleles were identified by confirming more than 30 promising loci and candidate genes on periodontal health and diseases. Based on these results, it is necessary to validate genetic association in other countries. Hence, the aim of the present study is to confirm genetic association reported between periodontitis and metabolic traits in a Korean cohort.

Materials and Methods

Study Subjects

The participants of this study were recruited from the Korean Genome and Epidemiology study (KoGES) project, a national project to perform genome epidemiology studies in cohorts of Korean dwellers and immigrants/emigrants (Kim et al. 2017). Among the KoGES cohorts, a public genetic information dataset was established by the Korean Association Resource Consortium (KARE) based on the Ansan–Anseong cohort. This cohort is biennially followed up in the ongoing KoGES project (Karns et al. 2012). The KARE dataset consists of individual SNP chip genotypes and the epidemiological/clinical phenotypes for study of the genetic components of the Korean public health. Written informed consent was obtained from all the participants included at the KoGES. The obtained KARE dataset was analyzed based on the standard (inclusion/exclusion) criteria (Cho et al. 2009). In short, subjects with the genotyping accuracy below 98% and high extent of missing genotype call rates (≥ 5%), as well as high heterozygosity (> 30%) or inconsistency in gender-based data were excluded from subsequent analysis. Furthermore, this study excluded individuals with tumor, as well as those individuals whose estimated identity-by-state values were high (> 0.80). Based on these factors of criteria, a total of 8842 participants were identified as eligible for inclusion and screened for the purpose of our study.

Study Phenotypes and Covariates

A study using the CGAS measured the phenotypes and covariates (Jeong et al. 2014). First, the current study investigated the general demographic data based on resident areas (Anseong or Ansan), gender, and age as the covariates. In the next step, height and body weight were analyzed to calculate body mass index (BMI). Subsequently, the waist circumference (WC), systolic and diastolic blood pressures (SBP and DBP), fasting plasma glucose levels (GLU0), high-density lipoprotein (HDL) cholesterol, and triglyceride (TG) were measured for the genetic association study.

Study Genotypes

A previous study used an approach for genotyping of the cohort population for the KARE study (Kim et al. 2017). Moreover, those researchers isolated most of the DNA samples from the peripheral blood of the participants and genotyped them using Affymetrix Gene-wide Human SNP array 5.0 (Affymetrix, Inc., Santa Clara, CA, USA). The current study postulated that genetic association can be discovered using the quality-control steps X of the genotypes. To test this assumption, this study applied the Bayesian Robust Linear Modeling with the Mahalanobis Distance genotyping algorithm to determine the call rate of the genotyping. Consequently, 352,227 SNPs had a missing genotype call rate below 0.1, a minor allele frequency greater than 0.01, and no deviation from Hardy–Weinberg equilibrium (p > 1 × 10−6). In addition, CGAS reported no population stratification between the Anseong and Ansan cohorts (Cho et al. 2009).

Statistical Analyses

Linear regression analysis was performed for the current genetic association study on the basis of residential area, gender, and age. Afterward, statistical analyses were performed using PLINK (version 1.07) (Purcell et al. 2007). This study determined the significant associations using an unadjusted p value (< 0.05).

Results

Genetic Association Study Between Periodontitis Genes and Metabolic Traits

To confirm genetic association between periodontitis genes and metabolic traits in the Korean population, this study investigated ten periodontitis genes known as genetic risk factors and as clinical characteristics of metabolic traits (see Tables 1 and 2) (Hong et al. 2015). Based on these data, gene regions were determined (see Table 3). A total 10,268 SNPs of ten targeted periodontitis genes were analyzed among 2649 SNPs in five genes (TENM2, LDLRAD4, SLC9C2, MFSD1, and A2BP1), and their statistical differences (p < 0.05) are listed (Supplementary Table 1). In terms of the association with BMI, waist circumference (WAIST), and fasting glucose (FG), 2592 of 2,6S9 SNPs were located in A2BP1 and showed a statistical significance (p < 0.01) with respect to the obesity-phenotypes. Also, the presented study found that one SNP (rs138566395) showed statistical significance at p < 0.01 (see Table 4A) in the relationship between triglycerides (TG) and high-density lipoprotein (HDL) cholesterol. Interestingly, these SNPs were related to BMI (β ± SE = − 0.23 ± 0.06, p = 0.00022) and WAIST and HDL (see Tables 4A and 4C).

Table 2.

Characteristics of clinical metabolic traits

List Cohort The analysis model
No. of individuals 8842
Resident area (Anseong %) 47.5 Covariates
Gender (male %) 47.3 Covariates
Age (years old) 52.2 ± 8.9 Covariates
Height (cm) 160.0 ± 8.7
Body mass index (kg/m2) 24.6 ± 3.1 Target 1
Fasting glucose (mg/dL) 87.7 ± 21.9 Target 2
DBP (mmHg) 80.3 ± 11.5 Target 3
SBP (mmHg) 121.7 ± 18.6 Target 4
Waist circumference (cm) 82.7 ± 8.8 Target 5
HDL cholesterol (mg/dL) 44.7 ± 10.1 Target 6
Triglyceride (mg/dL) 162.9 ± 105.7 Target 7
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Table 3.

Targeted gene regions

Periodontitis genes Chr Gene regions Analysis region (gene region ± 5 kbp)
Start End Start End SNPs
TENM2 5 166711843 167691162 166706843 167696162 103
LDLRAD4 18 13218729 13652753 13213729 13657753 77
SLC9C2 1 173469604 173572233 173464604 173577233 245
RASGRP4 19 38899698 38916945 38894698 38921945 95
MFSD1 3 158519715 158547508 158514715 158552508 105
IL4 5 132009678 132018370 132004678 132023370 48
NMUR2 5 151771102 151784840 151766102 151789840 115
GPR141 7 37723378 37783423 37718378 37788423 198
BLK 8 11351501 11422113 11346501 11427113 176
A2BP1 (RBFOX1) 16 6069132 7763340 6064132 7768340 9106
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Table 4.

Adjusted association of genomewide association study between periodontitis genes and metabolic traits

(A) Genomewide association with body mass index (BMI), waist circumference (WAIST), and fasting glucose (FG)
CHR SNP BP A1 NMISS BMI SE P WAIST SE P FG SE P
A2BP1 16 rs138566395 7323241 C 8836 − 0.23 0.06 2.20E−04 − 0.61 0.17 3.50E−04 − 0.69 0.47 1.40E−01
TENM2 5 rs1529682 167486071 G 8831 − 0.33 0.15 2.50E−02 − 1.19 0.4 2.90E−03 1.25 1.11 2.60E−01
TENM2 5 rs1477284 167217586 T 8835 − 0.15 0.06 9.90E−03 − 0.33 0.16 3.90E−02 0.1 0.44 8.20E−01
TENM2 5 rs3733986 167585972 T 8834 − 0.05 0.05 3.40E−01 − 0.08 0.13 5.60E−01 − 1.24 0.37 8.30E−04
(B) Genomewide association with diastolic blood pressure (DBP), and systolic blood pressure (SBP)
CHR SNP BP A1 NMISS DBP SE P SBP SE P
LDLRAD4 18 rs3132835 13496046 C 8836 0.53 0.19 5.10E−03 0.81 0.29 4.60E−03
MFSD1 3 rs2061617 158523819 G 8836 2.33 0.73 1.50E−03 2.47 1.1 2.50E−02
SLC9C2 1 rs190870441 173568389 G 8836 − 1.45 0.68 3.30E−02 − 3.03 1.02 3.00E−03
(C) Genomewide association with triglycerides (TG) and high-density lipoprotein (HDL) cholesterol
CHR SNP BP A1 NMISS TG SE P HDL SE P
A2BP1 16 rs138566395 7323241 C 8836 − 4.77 2.21 3.10E−02 0.64 0.24 7.10E−03
LDLRAD4 18 rs3931961 13393109 C 8830 − 4.87 1.71 4.40E−03 0.33 0.18 7.40E−02
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Next, this study also showed that three SNPs (rs1529682, rs147728, and rs3733986) of TENM2 were associated with BMI, WAIST, and FG (see Table 4A). Moreover, LDLRAD4, SLC9C2, and MFSD1 were related to DBP and SBP in triglycerides (TG) and high-density lipoprotein cholesterol (see Table 4B).

Discussion

The present study discovered that five genes (A2BP1, TENM2, LDLRAD4, SLC9C2, and MFSD1) were associated with metabolic traits such as obesity and high blood pressures. Also, these genes were strongly associated with BMI (p = 2.2 × 10−4). However, when either Bonferroni correction or false discovery rate correction was applied, no statistical significance was observed. This may be the reason that metabolic traits can be determined by age, gender, socioeconomic status, genetic factor, and lifestyle. Based on these facts, the present study focused on investigating the genetic association between periodontitis genes and metabolic traits.

This study demonstrated that A2BP1 was strongly associated with periodontitis. Therefore, this gene could be considered as a genetic risk marker. In the United States, A2BP1 is already reported as a genetic risk marker (Purcell et al. 2007). A2BP1 encoding Ataxin 2-binding protein 1 is known as RNA-binding fox-1 Homolog 1. Reduction of this gene in mouse hypothalamus cells led to decreases in the expressions of ATXN2, INSR, and MC4R, which have important roles in the metabolic pathways (Ma et al. 2010). Conversely, increased A2BP1 expression was found in obesity, Also, this gene interacted with ATXN2, INSR, and MC4R, which played important roles in metabolic pathways (Ma et al. 2010). Interestingly, rs138566395 on third intron of the gene in the A2BP1 region showed significant associations with BMI, HDL cholesterol, Triglycerides, and WAIST (see Table 4).

TENM2 encoding teneurin-transmembrane protein 2 was strongly associated with periodontitis. In line with this finding, a recent study showed that TENM2 was dramatically increased in the adipocyte progenitor cells. Moreover, TENM2-deficiency upregulated brown adipocyte marker genes (Tews et al. 2017), which indicated that TENM2 could contribute to cell fate. TENM2 was reported to act as a membrane-bound transcriptional regulator in the intracellular role and inhibit zic-mediated transcription by stimulating the apolipoprotein E (APOE) promoter (Bagutti et al. 2003). Induction of APOE was observed in the patients with Porphyromonas gingivalis (Lei et al. 2013), which indicated that periodontitis might induce metabolic syndrome via TENM2-activated APOE promoter. In addition, it was reported that TENM2 was related to subgingival Aggregatibacter actinomycetemcomitans known as one of the periodontal pathogens (Divaris et al. 2012). Besides, this gene was important in maintaining serum uric acid concentration (Karns et al. 2012).

LDLRAD4, MFSD1, and SLC9C2 were strongly associated with DBP and SBP. Particularly, LDLRAD4 was observed in the patients with blood pressure (SBP and DBP). Reportedly, this gene consisted of low-density lipoprotein receptor (LDLR) class A domains which are the major cholesterol-carrying lipoproteins of plasma. Besides, LDL binds class A domain to the LDLR, and then transports it into cells (Van der Horst et al. 2009). Therefore, the LDLRAD4 was critical in cholesterol homeostasis in mammalian cells. In addition, because the LDLR class A domain is a binding site for calcium, numerous familial hypercholesterolemia mutations of the LDL receptor could alter the calcium-coordinating residue of LDL-A domains (Yamamoto and Yamashita 1998). Therefore, a significant upregulation of LDLRAD4 in periodontitis could disrupt intracellular homeostasis of calcium. Based on these facts, this study suggested that LDLRAD4 expression was important for maintaining cholesterol homeostasis in mammalian cells.

In summary, the present study showed that gene triggering periodontitis was related to obesity and blood pressure using the analysis of SNPs. Specifically, if any one of TENM2, A2BP1, LDLRAD4, SLC9C2, or MFSD1 was observed in the patients with periodontitis, obesity and blood pressure have to be treated simultaneously.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (XLSX 624 kb) (624KB, xlsx)

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest.

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