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. Author manuscript; available in PMC: 2019 Jun 9.
Published in final edited form as: Nature. 2018 Nov 21;563(7732):559–563. doi: 10.1038/s41586-018-0702-1

Extended Data Fig. 8 |. Expression and purification of MPRLV VLPs and their interaction with soluble PCDH1.

Extended Data Fig. 8 |

a, Purified MPRLV VLPs (8 μg protein) bearing an N-terminal StrepTag were resolved on an SDS–polyacrylamide gel and visualized by Coomassie blue staining. R, reducing conditions; NR, nonreducing conditions. b, MPRLV VLPs were negatively stained with uranyl acetate and visualized by electron microscopy. c, Kinetic constants for MPRLV VLP-sEC1–2 interaction were determined by biolayer interferometry. kon, association-rate constant; koff, dissociation rate constant. Values ± 95% confidence intervals derived from curve fits are shown for two independent experiments. These experiments were performed three times with similar results.