Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic Reagent (Drosophila melanogaster) | GluRIII[RNAi] or UAS-GluRIII[RNAi] | PMID: 25859184 | FlyBase ID:FlyBase_FBtp0110520 | UAS-pWiz transgene knocking downGluRIII gene function. This lab (CAF) is the source (Brusich et al., 2015). |
| Genetic Reagent (D. melanogaster) | Plc21C[RNAi] or UAS-Plc21C[RNAi] | Vienna Drosophila Resource Center (GD11359); PMID: 17625558 | RRID:FlyBase_FBst0456476 | UAS-RNAi transgene |
| Genetic Reagent (D. melanogaster) |
Plc21C[RNAi] or UAS-Plc21C[RNAi] | Vienna Drosophila Resource Center (GD11359); PMID: 17625558 | RRID:FlyBase_FBst0456477 | UAS-RNAi transgene |
| Genetic Reagent (D. melanogaster) | UAS-IP3-sponge.m30 | PMID: 16540404 | FlyBase ID:FlyBase_FBtp0068098 | also referred to as UAS-IP3-sponge |
| Genetic Reagent (D. melanogaster) | UAS-IP3-sponge.m49 | PMID: 16540404 | FlyBase ID:FlyBase_FBtp0068099 | also referred to as UAS-IP3-sponge |
| Genetic Reagent (D. melanogaster) | GluRIIASP16 | PMID: 9427247 | RRID:BDSC_64202 | deletion allele; also referred to as GluRIIA |
| Genetic Reagent (D. melanogaster) | w1118 | PMID: 6319027 | RRID:BDSC_3605 | wild-type genetic background |
| Genetic Reagent (D. melanogaster) | elaV(C155)-Gal4 | PMID: 7917288 | RRID:BDSC_458 | also known as C155-Gal4 |
| Genetic Reagent (D. melanogaster) | Sca-Gal4 | PMID: 8893021 | FlyBase ID:FlyBase_FBtp0007534 | |
| Genetic Reagent (D. melanogaster) | BG57-Gal4 | PMID: 8893021 | FlyBase ID:FlyBase_FBti0016293 | also known as C57-Gal4 |
| Chemical Compound, Drug | Philanthotoxin-433; PhTox | Sigma-Aldrich (MilliporeSigma); Santa Cruz Biotechnology | CAS Number:(Sigma-Aldrich and Santa Cruz Biotechnology)_276684-27-6 | product P207 discontinued by Sigma-Aldrich |
| Chemical Compound, Drug | Xestospongin C | Abcam | CAS Number:Abcam_88903-69-9 | |
| Chemical Compound, Drug | 2-APB | Tocris | CAS Number:Tocris_524-95-8 | |
| Chemical Compound, Drug | Ryanodine | Tocris | CAS Number:Tocris_15662-33-6 | |
| Chemical Compound, Drug | Dantrolene | Tocris | CAS Number:Tocris_14663-23-1 | |
| Antibody | Monoclonal mouse anti-Synapsin | DSHB (3C11) | Cat#: DSHB_3C11; RRID:AB_2313867 | (1:50) |
| Antibody | Polyclonal rabbit anti-Dlg | PMID: 8893021 | (1:15,000) | |
| Antibody | Polyclonal goat anti-mouse 488 (DyLight) | Jackson ImmunoResearch | Cat #:Jackson_ 115-485-003; (no RRID) | (1:1000) discontinued; substitute with Cat# 115-485-068; RRID:AB_2338804 |
| Antibody | Polyclonal goat anti-rabbit 549 (DyLight) | Jackson ImmunoResearch | Cat#:Jackson_111-505-003; RRID:AB_2493180 | (1:2000) discontinued; substitute with Cat# 111-165-003; RRID:AB_2338000 |
| Antibody | Polyclonal goat anti-HRP (Alexa-647) | Jackson ImmunoResearch | Cat#:Jackson_123-605-021; RRID:AB_2338967 | (1:250) |
| Software, Algorithm | pClamp | Molecular Devices | RRID:SCR_011323 | |
| Software, Algorithm | MiniAnalysis Program | Synaptosoft | RRID:SCR_002184 | |
| Software, Algorithm | GraphPad Prism | GraphPad | RRID:SCR_002798 |