Fig. 2.

In situ Hybridisation and Immunohistochemical Analysis of Skin-biopsies with JMTV Infection. In situ RNA hybridisation using JMTV-segment 2 probe sets (Quasar 570) on formalin-fixed, paraffin-embedded (FFPE) skin eschar of Patient 1. Nuclei were counterstained with DAPI (blue). Magnification×200, insert×400 (Panel A). Haematoxylin and eosin staining, and immunophenotyping of lymphocytes (CD3), neutrophils (CD15) and macrophages (CD68) on serial sections of Patients 1–3. Magnification×200, counterstained with haematoxylin, peroxidase immunostaining in brown (Panel B). No FFPE specimen was available for Patient 4. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)