CELL BIOLOGY Correction for “Acquisition of a hybrid E/M state is essential for tumorigenicity of basal breast cancer cells,” by Cornelia Kröger, Alexander Afeyan, Jasmin Mraz, Elinor Ng Eaton, Ferenc Reinhardt, Yevgenia L. Khodor, Prathapan Thiru, Brian Bierie, Xin Ye, Christopher B. Burge, and Robert A. Weinberg, which was first published March 25, 2019; 10.1073/pnas.1812876116 (Proc. Natl. Acad. Sci. U.S.A. 116, 7353–7362).
The authors wish to note the following: “During the assembly of this manuscript, the authors inadvertently placed duplicated images of the hematoxylin and eosin (H&E) staining of the E-SCC-Zeb1KO tumors of Fig. 2D into Fig. 3E E-SCC-Zeb1KO and E-SCC-ZEB1KOSn. The duplicate images have now been replaced with the originally intended images. This correction does not affect any result described in the figures and does not alter the message of the manuscript in any way. We apologize for any inconvenience that this error may have caused readers.” The corrected Fig. 3 and its legend appear below.
Fig. 3.
Zeb1 is needed for a complete EMT, but the hybrid E/M cell state is sufficient for tumor formation. (A) Representative images of cell morphology of E-SCC, E-SCCSn, E-SCC-Zeb1KO, and E-SCC-Zeb1KOSn cells by phase contrast (brightfield) microscopy. (B) FACS profiles for CD104 and CD44 of E-SCC, E-SCCSn, E-SCC-Zeb1KO, and E-SCC-Zeb1KOSn populations. (C) Assessment of tumorigenicity and tumor growth by tumor weight of orthotopic injection E-SCC, E-SCCSn, E-SCC-Zeb1KO, and E-SCC-Zeb1KOSn populations. Data are presented as mean ± SEM (D) Differences in tumor-initiating ability of E-SCC, E-SCCSn, E-SCC-Zeb1KO, and E-SCC-Zeb1KOSn cells upon transplantation at limiting dilutions into NOD/SCID mice. (E) Analysis of E-SCC, E-SCCSn, E-SCC-Zeb1KO, and E-SCC-Zeb1KOSn tumor sections using H&E and IF staining for Krt/Zeb1 or Snail. LgT staining was used to differentiate tumor cells from mouse stromal cells. Nucleus is visualized by DAPI staining. (Scale bars, brightfield; H&E, 10 µm; IF, 2 µm in E.)