Skip to main content
NCBI home page
Toxicology Reports logoLink to Toxicology Reports
. 2019 Jun 7;6:505–513. doi: 10.1016/j.toxrep.2019.06.001

Ameliorative effect of gallic acid in paclitaxel-induced neuropathic pain in mice

Satbir Kaur a, Arunachalam Muthuraman b,c,d,⁎,1
PMCID: PMC6562321  PMID: 31211096

Graphical abstract

graphic file with name ga1.jpg

Open in a new tab

Keywords: Allodynia, Gallic acid, Hyperalgesia, Myeloperoxidase, Paclitaxel, Sciatic nerve

Highlights

  • •

    Gallic acid (GA) is a natural phenolic type of neuroprotective compound.

  • •

    GA possesses anti-nociceptive action against paclitaxel-induced neurotoxicity.

  • •

    GA inhibits THF-α mediated neuropathic pain sensation.

Abstract

The present study has been investigated the role of gallic acid (GA) in paclitaxel-induced neuropathic pain. The neuropathic pain was developed with paclitaxel (PT: 2 mg/kg, i.p.) administration in mice. GA (20 and 40 mg/kg) and pregabalin (PreG: 5 mg/kg) were administered intravenously for 10 consecutive days. The neuralgic sensations were investigated by assessing various pain tests like acetone drop, pinprick, plantar, tail flick, and tail pinch test. Mice pain behaviors were evaluated on 0, 4th, 8th, 12th and 16th days. The levels of sciatic nerve thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), superoxide anion, calcium, myeloperoxidase (MPO), and TNF-α were estimated. Treatment of GA and PreG attenuate PT induced thermal &mechanical hyperalgesia and allodynia symptoms along with the reduction of TBARS, total calcium, TNF-α, superoxide anion, and MPO activity levels; and decreased GSH level. Therefore, it has been concluded that GA has potential neuroprotective actions against PT induced neuropathic pain due to it's anti-oxidant, anti-inflammation and regulation of intracellular calcium ion concentration.

1. Introduction

Paclitaxel (PT) is a potent cancer chemotherapeutic agent and it is used widely for the management of various cancers i.e., breast, lung and ovarian cancer. Clinically, PT is indicated to the management of multiple cancer disorders [1]. Unfortunately, it causes neurodegeneration of a peripheral nerve ending, leading to progress the painful neuropathy [2]. The primary toxic mechanism of PT has altered the production of microtubulin polymerization via synthesis of free radicals, TNF-α, and BCL2 proteins; alteration of cellular pro & anti-oxidant enzymes; calcium dyshomeostasis; and the opening of mitochondrial permeability transition pores (MPTP) [[3], [4], [5]]. Experimentally, administration of PT documented to produce the neuropathic pain symptoms in rodents as well as in humans [6,7]. However, the avoidance of PT prescription is challenging because it has higher efficacy, potency, improvement of the quality of life and affordable price (cheaper) for cancer patients [8]. Therefore, the management of PT associated neurodegenerative process and neuropathic pain symptoms are an emerging field in neurological research.

Neuropathic pain is not a symptom; it is a disease itself. Neurodegeneration of the central and peripheral nervous system causes neuropathic pain. An indication of neuropathic pains is unpleasant, painful sensation and/or lack of sensation [9]. Management of neuropathic pain disorder is very complicated due to multiple complex mechanisms, and available conventional drugs produce only symptomatic relief of neuropathic pain. The most recommended medicines for neuropathic pain are narcotic i.e., morphine and codeine; anti-depressants i.e., desipramine and imipramine; and anti-epileptic drugs like carbamazepine, gabapentin, and phenytoin [10,11]. Paradoxically, these agents have shown less satisfactory action on neuropathic pain and also produce potential toxic effects [[12], [13], [14]]. Therefore, the identification of safer alternatives is urgently needed for the mitigation of painful neuropathy. Various reports have suggested that natural products like herbal and nutritional plants play a key role in the management of neuropathic pain and wellness of neurological functions [15,16]. The primary benefits of these molecules are the lack of adverse effects with chronic use and attractive cost-effect relationships.

Moreover, some of the plant extracts of Acorus calamus, Ocimum sanctum, Butea monosperma, Citrullus colocynthis, Ginkgo biloba, Curcuma longa, Mitragyna speciosa, Phyllanthus amarus and Salvia officinalis prevent neuropathic pain [17]. In addition, phytoconstituents e.g., celastrol [18], liquiritigenin [19], tocotrienol [20], lycopene [21], thymoquinone [22], epigallocatechin gallate [23] and resveratrol [24] also produce anti-neuralgic action. Gallic acid (GA) is a polyphenolic secondary metabolite of various plants [25,26]. GA also produces anti-oxidant action in a biological system [27]. In addition, plant-derived natural products are capable to produce the cytotoxic (cancer cell) [28], and chemo-preventive actions [29].

GA is a primary anti-oxidant component of tea extract. Ayurvedic herbs have large quantities of GA [26,30,31]. Experimentally, GA has been documented to produce anti-hyperlipidemic, cardioprotective, anti-diabetic activity [32,33]. Furthermore, GA has potential neuroprotective action due to mono and poly-targeted actions [34,35]. In addition, an acute and sub-acute toxicity study of GA (5000 mg/kg; per oral) documented that, GA did not produce any serious adverse effects and organ toxicity [36]. Therefore, GA may be recommended for use in chronic neurodegenerative disorders due to its higher safety margin. However, the experimental evidence of GA in neuropathic pain disorder is limited. Earlier, pregabalin (PreG) is widely employed to manage the seizure attacks; nowadays, it used for the management of diabetic and postherpetic neuropathic pain [37]. The exact molecular mechanism of PreGhas not been fully explored. However, it is reported to act via reduction of presynaptic neurotransmitter release, like substance P and glutamate, via neuronal N‐type calcium channel blocking action [37,38]. Experimentally, PreG produces potent neuropathic pain relieving action. Hence, PreG was used as a reference control in this study. Based on this review of the literature, the present study has been investigated the therapeutic role of GA in PT induced neuropathic pain in mice.

2. Materials and methods

2.1. Animals

About 20–25 g weight, age of 10-month-old and disease free male Swiss albino mice were used for evaluation of GA in PT induced neuropathic pain. Animals were kept at standard laboratory diet, temperature (65–75 °F; ˜18-23 °C) and humidity (40–60 %) condition. All animals were kept one week in laboratory condition for acclimatization with 12 h light-dark cycle. The mice were allowed to take the rodent diet and water spontaneously (ad libitum). The experimental design was accepted by the Institutional Animal Ethics Committee (IAEC) to carry out this experimental design. IAEC approval No is ATRC/09/I4. The care of mice was taken as per IAEC and Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA; Ministry of Environment and Forest, Government of India) guidelines.

2.2. Drugs and chemicals

Sigma Aldrich (Mumbai, India) company was supplied thiobarbituric acid (TBA) and nitro-blue tetrazolium (NBT). The N-naphthylethylenediamine; sulfanilamide; 1,1,3,3-tetramethoxy propane and GA chemicals were supplied by Sisco Research Laboratories, Mumbai. The rat specific TNF-alpha ELISA kit was procured for TNF-alpha estimation from RayBio, Inc., USA. The rest of the chemical were purchased with an analytical grade from SD Fine Chemicals, Mumbai, India.

2.3. Development of neuropathic pain in mice

The progress of neuropathic pain in mice was achieved by intraperitoneal administration of PT (2 mg/kg; for 5 days) [6,7,39]. Nociceptive pain threshold was assessed at different time points like 0, 4th, 8th, 12th and 16th days.

2.4. Experimental protocol

The present study was comprised six experimental groups. Each group comprises eight Swiss albino mice (n = 8). Group I (Control): Mice were not subjected to any drug or vehicle administration. GA and PT were soluble in water. Water is one of the common solvent (or vehicle) and it doesn’t have any pain modulating action. Therefore, a separate vehicle treatment in PT treated animal and vehicle treatment in normal animals not included in this experimental design. Group II (GA per se): Mice received an intravenous dose of GA (40 mg/kg) for 10 days (from day 6) in mice. Group III (PT): PT (2 mg/kg) was administrated intraperitoneally for 5 days. Group IV and V (GA; 20 and 40 mg/kg): Mice received an intravenous dose GA (20 and 40 mg/kg) for 10 days from day 6 respectively. The 10 min time intervals were maintained between each administration of GA and PT. Group VI (PreG; 5 mg/kg): Mice received an intravenous dose PreG (5 mg/Kg) for 10 days from day 6. All six groups were involved in the assessment of pain behavior assessment and biochemical estimations. Nociceptive pain threshold was assessed at different time points like 0, 4th, 8th, 12th and 16th days. The end of the study period i.e., 16th day, mice were sacrificed after the assessment of behavioral tests. The samples of the sciatic nerve and surrounding muscular tissues were collected for the estimation of biomarkers levels. Experimental protocol design has been illustrated in Fig. 1.

Fig. 1.

Fig. 1

Open in a new tab

Experimental protocol and treatment schedules of GA and PT. Abbreviation: GA, gallic acid, PT, paclitaxel; and PreG, pregabalin.

2.5. Behavioral evaluation

2.5.1. Acetone drop test

Thermal (cold) chemical sensitivity was assessed in the right hind paw by the application of acetone drop as per Choi et al. method [40]. Clinically, it similar to chemical (thermal) allodynic symptoms. Briefly, mice were placed in a closed rectangle wire mesh chamber and about 100 μl of acetone was sprinkled at the plantar surface of the right hind paw. The one-minute duration was allowed to observe the chemical cold sensations. This cold sensation was scored as score 1 for paw licking response; score 2 for shaking response; score 3 for lifting duration of the right hind paw within 4 s; score 4 for lifting duration of right hind paw between 5–8 seconds; and score 5 for lifting duration of right hind paw above 8. A total score i.e., 15 were considered as noted as severe neuronal injury. And, the lowest score was considered as neuroprotection.

2.5.2. Pin prick test

The mechanical pain sensation of mice was evaluated as described by Erichsen and Blackburn-Munro [41]. Clinically, it resembles pinpoint mechanical hyperalgesic symptoms. The blunted needle was applied (touch sensation) perpendicularly to the right hind paw plantar (mid) surface. The intensity was generated from the detection of brisk withdrawal reaction of the right hind paw. The interval of needle application was maintained six times per minute. The brisk withdrawal of the hind limb was indicated as a painful response. The stimuli were applied in the mid-plantar surface only six times per minutes to avoid the unwanted tissue injury and developments of the wind-up phenomenon.

2.5.3. Plantar test

Mice were engaged to receive the radiant heat stimuli in the right hind paw as described by Hargreaves et al. [42]. Clinically, it resembles thermal hyperalgesic symptoms. The right hind paw was located on the slit of the plantar device. Brisk withdrawal of the right hind limb was noted as a thermal hyperalgesic response. Cut off time i.e., 20 s was maintained to avoid potential tissue damage.

2.5.4. Tail flick test

The thermal (radiant heat) sensation of mice tail part was assessed by observation of tail flick response as per D’Aemour and Smith method [43] with minor changes of Hargreaves et al. [42]. Clinically, it resembles central sensations. The one-centimeter distance from the tail terminal region was located on the slit of the plantar device. Thermal sensitivity of mice tail was noted as tail withdrawal latency. Brisk withdrawal of tail from the plantar device was considered as a progression of neuropathic pain. Cut off stimuli i.e., 15 s was maintained to avoid potential tissue damage.

2.5.5. Tail pinch test

The mechanical pain sensation of mice tail was assessed by observation of tail clamp dislodgment attempts of mice as per Takagi et al. method [44] with minor changes of Moon et al. [45] and Deshmukh et al. [46]. Clinically, it similar to mechanical compression type of pain sensation. Hoffmann clamp was applied near the base of the mice tail and screw was adjusted to make the mechanical compression type of pain within 5 s. An increasing of dislodgment attempts on Hoffmann clamp was noted as a progression of neuropathic pain. Cut-off time i.e., 10 s was maintained to avoid potential tissue damage.

2.6. Biochemical estimation

The isolated tissue samples were maintained in the humidity chamber at 85 percentage of relative humidity. The sciatic nerve homogenate (10% w/v) was prepared with 0.1 M Tris−HCl buffer (pH 7.4); deionised water; and phosphate buffer (pH 7.4) for total protein, thiobarbituric acid reactive substances (TBARS) & reduced glutathione (GSH); total calcium; and tumor necrosis factor-alpha (TNF-α) estimation respectively. Superoxide anion and myeloperoxidase (MPO) activity were estimated from a muscular tissue sample.

2.6.1. Quantification of thiobarbituric acid reactive substances (TBARS)

TBARS was quantified as described by Ohkawa et al. [47]. Absorbance was recorded by a spectrophotometer (UV-1800 UV–vis spectrophotometer, SHIMADZU Corporation, Tokyo, Japan). The wavelength was fixed with 535 nm for estimation of TBARS. A standard plot was made with reference molecules i.e., 1–10 nanomole (nM) of 1, 1, 3, 3-tetramethoxy propane. The results of tissue TBARS levels were indicated as nM of malondialdehyde (MDA) per milligram (mg) of protein.

2.6.2. Quantification of reduced glutathione (GSH)

GSH level was quantified as a described by Beutler et al. [48]. Absorbance was recorded by a spectrophotometer. The wavelength was fixed with 412 for estimation of GSH. A standard plot was made with reference molecules i.e., 10–100 microgram (μg) of GSH. The results of tissue GSH levels were indicated as microgram (μg) of GSH per milligram (mg) of protein.

2.6.3. Quantification of total calcium

Total calcium level was quantified as a described method of Severnghaus and Ferrebee [49] with minor changes of Muthuraman et al. [50]. Absorbance was recorded by a spectrophotometer at 556 nm wavelength. A standard plot was made with reference molecules i.e., 100–1000 parts per million (ppm) of calcium chloride. Results of total calcium were expressed as ppm per milligram (mg) of sciatic nerve tissue.

2.6.4. Quantification of tumor necrosis factor-alpha (TNF-α)

The TNF-α level was quantified in sciatic nerve homogenate as described by Muthuraman et al. [51]. Absorbance was recorded by a spectrophotometer at 450 nm wavelength. A standard plot was made with reference molecules i.e., 0–20,000 pg per ml of reference standard i.e., TNF-α kit sample. Results of TNF-α level was indicated as pictogram (pg) of TNF-α per milligram (mg) of total protein.

2.6.5. Quantification of superoxide anion generation

Superoxide anion generation level was quantified by Wang et al. method [52] with minor changes of Muthuraman and Singh [53]. Absorbance was recorded by a spectrophotometer at 540 nm wavelength. Results of NBT reduction level was indicated as picomole (pm) per minute per milligram (mg) of the sciatic nerve.

2.6.6. Quantification of myeloperoxidase (MPO) activity

Myeloperoxidase activity level was quantified by a method of Patriarca et al. [54] with a minor change of Grisham et al. method [55]. Absorbance was recorded by a spectrophotometer at 460 nm wavelength. Results of MPO activity level was indicated as units per milligram (mg) of protein per minute.

2.6.7. Quantification of total protein

Total protein level was quantified by a method of Lowry’s et al. [56]. Absorbance was recorded by a spectrophotometer at 750 nm wavelength. A standard plot was made with reference molecules i.e., 1–10 mg of bovine serum albumin. Results of total protein level were indicated as mg per ml of supernatant.

2.7. Statistical analysis

The behavioral and biochemical data were indicated as mean ± standard deviation (SD). Data of pain response tests were analyzed with two-way analysis of variance (ANOVA) via Bonferroni's post-hoc analysis were applied by utilizing Graph pad prism software (Version-5.0). in addition, tissue biochemical changes i.e., TBARS, GSH, calcium, TNF-α level, superoxide anion, and myeloperoxidase levels were analyzed with one way ANOVA via Tukey’s post-hoc analysis by utilizing SigmaStatVersion-3.5 software. A probability value i.e., a p-value less than 0.05 was considered statistically significant.

3. Results

3.1. Role of GA in an acetone drop test

The intraperitoneal administration of PT (2 mg/kg) resulted in significant (p < 0.05) rise thermal allodynic sensation as an indication of increasing the scoring of chemical sensation dissimilarity to the control group. Administration of GA (20 and 40 mg/kg, i.v.) attenuated PT induced increase in the scoring of chemical sensation with concentration-dependent manner. Effect of GA is comparable and similar to the PreG treatment group. However, GA (40 mg/kg; i.v.) per se treated group did not show any significant (p < 0.05) changes in PT induced thermal allodynia (Fig. 2).

Fig. 2.

Fig. 2

Open in a new tab

Role of GA in acetone drop test (paw thermal allodynia). Digits in parenthesis indicate dose in mg/kg. Data were expressed as mean ± SD, n = 6 mice per group. *p < 0.05 when compared to control group. #p < 0.05 when compared to the PT group. Abbreviation: GA, gallic acid, PT, paclitaxel; and PreG, pregabalin.

3.2. Role of GA in the pinprick test

The intraperitoneal administration of PT (2 mg/kg) resulted in significant (p < 0.05) rise mechanical hyperalgesic sensation as an indication of an increase in the percentage paw withdrawal level when compared to control group. Administration of GA (20 and 40 mg/kg, i.v.) attenuated PT induced increase in the paw withdrawal response with concentration-dependent manner. Effect of GA is comparable and similar to the PreG treatment group. However, GA (40 mg/kg; i.v.) per se treated group did not show any significant (p < 0.05) changes in PT induced mechanical hyperalgesia (Fig. 3).

Fig. 3.

Fig. 3

Open in a new tab

Role of GA in the pinprick test (paw mechanical hyperalgesia).Digits in parenthesis indicate dose in mg/kg. Data were expressed as mean ± SD, n = 6 mice per group. *p < 0.05 when compared to control group. #p < 0.05 when compared to the PT group. Abbreviation: GA, gallic acid, PT, paclitaxel; and PreG, pregabalin.

3.3. Role of GA in plantar test

The intraperitoneal administration of PT (2 mg/kg) resulted in significant (p < 0.05) rise the thermal hyperalgesic sensation as an indication of a decrease in paw withdrawal threshold dissimilarity to control group. Administration of GA (20 and 40 mg/kg, i.v.) attenuated PT induced a decrease in paw withdrawal threshold with concentration-dependent manner. Effect of GA is comparable and similar to the PreG treatment group. However, GA (40 mg/kg; i.v.) per se treated group did not show any significant (p < 0.05) changes in PT induced thermal hyperalgesia (Fig. 4).

Fig. 4.

Fig. 4

Open in a new tab

Role of GA in plantar test (paw heat hyperalgesia).Digits in parenthesis indicate dose in mg/kg. Data were expressed as mean ± SD, n = 6 mice per group. *p < 0.05 when compared to control group. #p < 0.05 when compared to the PT group. Abbreviation: GA, gallic acid, PT, paclitaxel; and PreG, pregabalin.

3.4. Role of GA in tail flick test

The intraperitoneal administration of PT (2 mg/kg) resulted in significant (p < 0.05) rise thermal hyperalgesic sensation as an indication of a decrease tail withdrawal threshold dissimilarity to control group. Administration of GA (20 and 40 mg/kg, i.v.) attenuated PT induced decrease tail withdrawal threshold with concentration-dependent manner. Effect of GA is comparable and similar to the PreG treatment group. However, GA (40 mg/kg; i.v.) per se treated group did not show any significant (p < 0.05) changes in PT induced thermal hyperalgesia (Fig. 5).

Fig. 5.

Fig. 5

Open in a new tab

Role of GA in tail flick test (tail heat hyperalgesia). Digits in parenthesis indicate dose in mg/kg. Data were expressed as mean ± SD, n = 6 mice per group. *p < 0.05 when compared to control group. #p < 0.05 when compared to the PT group. Abbreviation: GA, gallic acid, PT, paclitaxel; and PreG, pregabalin.

3.5. Role of GA in tail pinch test

The intraperitoneal administration of PT (2 mg/kg, i.p. for 5 consecutive days) resulted in significant (p < 0.05) rise mechanical hyperalgesia as an indication of an increasing number of dislodgement of the Heffner’s clamp when compared to control group. Administration of GA (20 and 40 mg/kg, i.v.) attenuated PT induced increase in mechanical nociceptive pain threshold with concentration-dependent manner. Effect of GA is comparable and similar to the PreG treatment group. However, GA (40 mg/kg; i.v.) per se treated group did not show any significant (p < 0.05) changes in PT induced mechanical hyperalgesia (Fig. 6).

Fig. 6.

Fig. 6

Open in a new tab

Role of GA in tail pinch test (tall mechanical hyperalgesia). Digits in parenthesis indicate dose in mg/kg. Data were expressed as mean ± SD, n = 6 mice per group. *p < 0.05 when compared to control group. #p < 0.05 when compared to the PT group. Abbreviation: GA, gallic acid, PT, paclitaxel; and PreG, pregabalin.

3.6. Role of GA in tissue biomarker changes

The intraperitoneal administration of PT (2 mg/kg) resulted in significant (p < 0.05) rise TBARS, total calcium, TNF-α, superoxide anion & MPO levels; and a decrease in GSH content as an indication of oxidative stress, inflammation, and neuronal damage when compared to control group. Administration of GA (20 and 40 mg/kg, i.v.) attenuated PT induced changes of above tissue biomarkers with concentration-dependent manner. Effect of GA is comparable and similar to the PreG treatment group. However, GA (40 mg/kg; i.v.) per se treated group did not show any significant (p < 0.05) changes in PT induced tissue biomarker changes (Tables 1 and 2 ).

Table 1.

Role of GA in tissue biomarker changes.

Groups TBARS (nM / mg of protein) GSH (μg / mg of protein) Total calcium (ppm/mg of protein) TNF-α (pg/ mg of protein)
Control 3.96 ± 0.53 79.12 ± 1.94 3.81 ± 0.79 31.67 ± 0.32
GA per se 3.84 ± 0.71 77.93 ± 2.04 3.97 ± 0.63 32.52 ± 0.61
PT 9.02 ± 0.51* 38.61 ± 1.73* 25.47 ± 0.81* 77.73 ± 0.58*
PT + GA (20) 5.35 ± 0.29# 68.04 ± 1.93# 8.71 ± 0.93# 42.19 ± 0.47#
PT + GA (40) 4.15 ± 0.93# 74.19 ± 1.62# 5.17 ± 0.81# 34.81 ± 0.39#
PT + PreG (5) 3.99 ± 0.69# 78.92 ± 1.63# 3.49 ± 0.72# 30.92 ± 0.71#
Open in a new tab

Digits in parenthesis indicate dose in mg/kg. Data were expressed as mean ± SD, n = 6 mice per group. *p < 0.05 vs control group. #p < 0.05 vs PT control group. Abbreviation: GA, gallic acid, PT, paclitaxel; PreG, pregabalin; TBARS, thiobarbituric acid reactive substances; and GSH, reduced glutathione.

Table 2.

Role of GA in tissue biomarker changes.

Groups NBT reduction (pM / Min / mg of tissue) MPO (unit / Min /mg of protein)
Control 3.53 ± 1.73 14.81 ± 1.91
GA per se 3.27 ± 1.21 15.49 ± 1.17
PT 28.81 ± 0.78* 95.39 ± 1.94*
PT + GA (20) 9.91 ± 1.52# 43.79 ± 2.82#
PT + GA (40) 5.04 ± 1.17# 37.94 ± 2.61#
PT + PreG (5) 4.62 ± 0.71# 19.79 ± 1.85#
Open in a new tab

Digits in parenthesis indicate dose in mg/kg. Data were expressed as mean ± SD, n = 6 mice per group. *p < 0.05 vs control group. #p < 0.05 vs PT control group. Abbreviation: GA, gallic acid, PT, paclitaxel; PreG, pregabalin; NBT, nitroblue tetrazolium; and MPO, myeloperoxidase.

4. Discussion

The present study revealed that the intraperitoneal administration of PT (2 mg/kg) significantly (p < 0.05) produced neuropathic pain by accelerating thermal & mechanical hyperalgesia and allodynia in paw and tail regions. It indicates that PTis enhancing neuronal excitation and accelerating neuropathic pain impulses [57]. Moreover, PT treatment also raises the sciatic nerve TBARS, total calcium, TNF-α, NBT reduction, and MPO activity levels along with the reduction of reduced glutathione levels. Results indicate that PT induces neuronal biomarkers mediated neuropathic pain like activation free radical generation, lipid peroxidation, calcium dyshomeostasis, raise inflammatory mediators and neuroinflammation. The treatments of GA (20 and 40 mg/kg, i.v.) attenuated the PT induced pain behavior and biochemical changes. It indicates that GA possesses the natural potency for prevention of neuronal firing, neurodegeneration, and neuroinflammation.

PT associated progression of painful neuropathy is a widely used model for testing of chemotherapy-induced polyneuritis type of neuropathic pain [58]. The relevance of humans pathology is similar to that of complex regional pain syndrome (CRPS) [59,60]. PT causes neuroinflammation and neurodegeneration via accumulation of intracellular calcium (Ca2+) ion concentrations [61–62,63,64], release of inflammatory cytokines [11], and alteration of neuronal microtubulin & myelin proteins [65,66]. In addition, PT also alters the neurovascular system leading to enhance the neuropathic pain symptoms [67]. Our recent findings revealed that alteration of a neuronal microvascular system is primary cardinal signs for the damage and degeneration of the nervous system [[68], [69], [70]]. Furthermore, some of our studies and other laboratory reports recommended that plant extracts and nutritional products of Acorus calamus [51,70], Butea monosperma [71], Swietenia mahagoni [72], Ocimum sanctum [73] and Vernonia cinerea [74] mitigate neuropathic pain symptoms. Phytoconstituents like puerarin [75], bulleyaconitine A [76], thymoquinone, epigallocatechin gallate, lycopene and resveratrol [17] produced an anti-neuralgic effect. Experimentally, GA has shown inhibition of histamine release, free radical scavenging action; reduction of oxidative stress, neuroinflammation and cytokine production [77,78,79]. Furthermore, the modern treatment approaches i.e., plant-derived medicines and genetic therapies are contributing to the amelioration of neuroinflammation and neurodegeneration [80]. Present results also indicate GA attenuates PT induced neuropathic pain symptoms via reduction TBARS, tissue total calcium, TNF-α, superoxide anion, MPO levels and prevents the reduction of GSH level.

PT enhances neuronal free radical production. Abundant accumulation of cytosolic free radicals is known to activate lipid peroxidation of the neuronal membrane [33]. In the present study, lipid peroxidation products (TBARS) levels were estimated by a spectrophotometric method [47]. Recent methods like high-performance liquid chromatography, enzyme-linked immunosorbent assay, gas chromatography, and fluorescence are also used for the quantification of TBARS levels [81]. However, this method is very common and widely employed for the assessment of cell membrane lipid peroxidation levels [82]. Subsequent reaction of lipid peroxidation is also altered cellular endogenous anti-oxidant substances i.e., GSH in PT treated animals. Same results were obtained in this research work and other research laboratory research reports [83]. Thereafter, neuronal structure, receptor expression (up-down regulation) and neuronal ion channels proteins are altered leading to enhance resting membrane potential via ionic imbalance [57]. Major secondary messengers of cell signal i.e., calcium accumulation leads to enhance the mitochondrial-mediated neuroinflammation and neurodegeneration [84]. Moreover, neurodegeneration also occurs with the free radical associated acceleration of inflammatory and immunological reactions. PT induced inflammatory and immunological reactions are due to the accumulation of TNF-α and MPO levels [85,86]. The treatment of GA has ameliorative effects against PT induced biomarker changes.

From data in hand and literature reports, it is concluded that GA possesses potential ameliorating effect against PT induced neuropathic pain symptoms viz free radical scavenging, reduction of inflammatory cytokines and cytosolic calcium ion concentration.

Funding source

This research received no specific grant from any funding agency in the public, commercial or not-for-profit sectors.

Transparency document

Transparency document
mmc1.zip (2.7MB, zip)

Acknowledgments

The authors are thankful to Akal Toxicology Research Centre, A unit of Akal College of Pharmacy & Technical Education, Mastuana Sahib, Sangrur-148001, Punjab (India) for supporting this study and providing technical facilities for this work.

References

  • 1.Chowdhury M.R., Moshikur R.M., Wakabayashi R., Tahara Y., Kamiya N., Moniruzzaman M., Goto M. Ionic-liquid-based paclitaxel preparation: a new potential formulation for cancer treatment. Mol. Pharm. 2018;15:2484–2488. doi: 10.1021/acs.molpharmaceut.8b00305. [DOI] [PubMed] [Google Scholar]
  • 2.Griffiths L.A., Duggett N.A., Pitcher A.L., Flatters S.J. Evoked and ongoing pain-like behaviours in a rat model of paclitaxel-induced peripheral neuropathy. Pain Res. Manag. 2018;2018 doi: 10.1155/2018/8217613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Starobova H., Vetter I. Pathophysiology of chemotherapy-induced peripheral neuropathy. Front. Mol. Neurosci. 2017;10:174. doi: 10.3389/fnmol.2017.00174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Park H.J. Chemotherapy-induced peripheral neuropathic pain. Korean J. Anesthesiol. 2014;67:4–7. doi: 10.4097/kjae.2014.67.1.4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Doyle T., Chen Z., Muscoli C., Bryant L., Esposito E., Cuzzocrea S., Dagostino C., Ryerse J., Rausaria S., Kamadulski A., Neumann W.L., Salvemini D. Targeting the overproduction of peroxynitrite for the prevention and reversal of paclitaxel-induced neuropathic pain. J. Neurosci. 2012;32:6149–6160. doi: 10.1523/JNEUROSCI.6343-11.2012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Callizot N., Andriambeloson E., Glass J., Revel M., Ferro P., Cirillo R., Vitte P.A., Dreano M. Interleukin-6 protects against paclitaxel, cisplatin and vincristine-induced neuropathies without impairing chemotherapeutic activity. Cancer Chemother. Pharmacol. 2008;62:995–1007. doi: 10.1007/s00280-008-0689-7. [DOI] [PubMed] [Google Scholar]
  • 7.Parvathy S.S., Masocha W. Matrix metalloproteinase inhibitor COL-3 prevents the development of paclitaxel-induced hyperalgesia in mice. Med. Princ. Pract. 2013;22:35–41. doi: 10.1159/000341710. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Dranitsaris G., Zhu X., Adunlin G., Vincent M.D. Cost-effectiveness vs. affordability in the age of immuno-oncology cancer drugs. Expert Rev. Pharmacoecon. Outcomes Res. 2018;18:351–357. doi: 10.1080/14737167.2018.1467270. [DOI] [PubMed] [Google Scholar]
  • 9.Backonja M.M. Use of anticonvulsants for treatment of neuropathic pain. Neurology. 2002;59:S14–S17. doi: 10.1212/wnl.59.5_suppl_2.s14. [DOI] [PubMed] [Google Scholar]
  • 10.Kaye A.D., Cornett E.M., Hart B., Patil S., Pham A., Spalitta M., Mancuso K.F. Novel pharmacological nonopioid therapies in chronic pain. Curr. Pain Headache Rep. 2018;22:31. doi: 10.1007/s11916-018-0674-8. [DOI] [PubMed] [Google Scholar]
  • 11.Al-Mazidi S., Alotaibi M., Nedjadi T., Chaudhary A., Alzoghaibi M., Djouhri L. Blocking of cytokines signaling attenuates evoked and spontaneous neuropathic pain behaviours in the paclitaxel rat model of chemotherapy-induced neuropathy. Eur. J. Pain. 2018;22:810–821. doi: 10.1002/ejp.1169. [DOI] [PubMed] [Google Scholar]
  • 12.Tentolouris-Piperas V., Lee G., Reading J., O’keeffe A., Zakrzewska J., Cregg R. Adverse effects of anti-epileptics in trigeminal neuralgiform pain. Acta Neurol. Scand. 2018;137:566–574. doi: 10.1111/ane.12901. [DOI] [PubMed] [Google Scholar]
  • 13.Baldo B.A. Opioid analgesic drugs and serotonin toxicity (syndrome): mechanisms, animal models, and links to clinical effects. Arch. Toxicol. 2018;92:2457–2473. doi: 10.1007/s00204-018-2244-6. [DOI] [PubMed] [Google Scholar]
  • 14.Volkow N., Benveniste H., McLellan A.T. Use and misuse of opioids in chronic pain. Annu. Rev. Med. 2018;69:451–465. doi: 10.1146/annurev-med-011817-044739. [DOI] [PubMed] [Google Scholar]
  • 15.Fayemi P.O., Muchenje V., Yetim H., Ahhmed A. Targeting the pains of food insecurity and malnutrition among internally displaced persons with nutrient synergy and analgesics in organ meat. Food Res. Int. 2018;104:48–58. doi: 10.1016/j.foodres.2016.11.038. [DOI] [PubMed] [Google Scholar]
  • 16.Sammugam L., Pasupuleti V.R. Balanced diets in food systems: emerging trends and challenges for human health. Crit. Rev. Food Sci. Nutr. 2018:1–14. doi: 10.1080/10408398.2018.1468729. [DOI] [PubMed] [Google Scholar]
  • 17.Forouzanfar F., Hosseinzadeh H. Medicinal herbs in the treatment of neuropathic pain: a review. Iran. J. Basic Med. Sci. 2018;21:347–358. doi: 10.22038/IJBMS.2018.24026.6021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Yang L., Li Y., Ren J., Zhu C., Fu J., Lin D., Qiu Y. Celastrol attenuates inflammatory and neuropathic pain mediated by cannabinoid receptor type 2. Int. J. Mol. Sci. 2014;15:13637–13648. doi: 10.3390/ijms150813637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19.Chen L., Chen W., Qian X., Fang Y., Zhu N. Liquiritigenin alleviates mechanical and cold hyperalgesia in a rat neuropathic pain model. Sci. Rep. 2014;4:5676. doi: 10.1038/srep05676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 20.Kuhad A., Chopra K. Tocotrienol attenuates oxidative-nitrosative stress and inflammatory cascade in experimental model of diabetic neuropathy. Neuropharmacology. 2009;57:456–462. doi: 10.1016/j.neuropharm.2009.06.013. [DOI] [PubMed] [Google Scholar]
  • 21.Kuhad A., Sharma S., Chopra K. Lycopene attenuates thermal hyperalgesia in a diabetic mouse model of neuropathic pain. Eur. J. Pain. 2008;12:624–632. doi: 10.1016/j.ejpain.2007.10.008. [DOI] [PubMed] [Google Scholar]
  • 22.Amin B., Taheri M.M., Hosseinzadeh H. Effects of intraperitoneal thymoquinone on chronic neuropathic pain in rats. Planta Med. 2014;80:1269–1277. doi: 10.1055/s-0034-1383062. [DOI] [PubMed] [Google Scholar]
  • 23.Tiwari V., Kuhad A., Chopra K. Amelioration of functional, biochemical and molecular deficits by epigallocatechin gallate in experimental model of alcoholic neuropathy. Eur. J. Pain. 2011;15:286–292. doi: 10.1016/j.ejpain.2010.07.005. [DOI] [PubMed] [Google Scholar]
  • 24.Sharma S., Kulkarni S.K., Chopra K. Effect of resveratrol, a polyphenolic phytoalexin, on thermal hyperalgesia in a mouse model of diabetic neuropathic pain. Fundam. Clin. Pharmacol. 2007;21:89–94. doi: 10.1111/j.1472-8206.2006.00455.x. [DOI] [PubMed] [Google Scholar]
  • 25.Zhao X., Zhi Q.Q., Li J.Y., Keller N., He Z.M. The antioxidant gallic acid inhibits aflatoxin formation in Aspergillus flavus by modulating transcription factors FarB and CreA. Toxins. 2018;10:270. doi: 10.3390/toxins10070270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 26.Ye Y., Yan J., Cui J., Mao S., Li M., Liao X., Tong H. Dynamic changes in amino acids, catechins, caffeine and gallic acid in green tea during withering. J. Food Compost. Anal. 2018;66:98–108. [Google Scholar]
  • 27.Park H.H., Ko S.C., Oh G.W., Jang Y.M., Kim Y.M., Park W.S., Choi I.W., Jung W.K. Characterization and biological activity of PVA hydrogel containing chitooligosaccharides conjugated with gallic acid. Carbohydr. Polym. 2018;198:197–205. doi: 10.1016/j.carbpol.2018.06.070. [DOI] [PubMed] [Google Scholar]
  • 28.Stagos D., Amoutzias G.D., Matakos A., Spyrou A., Tsatsakis A.M., Kouretas D. Chemoprevention of liver cancer by plant polyphenols. Food Chem. Toxicol. 2012;50:2155–2170. doi: 10.1016/j.fct.2012.04.002. [DOI] [PubMed] [Google Scholar]
  • 29.Sani T.A., Mohammadpour E., Mohammadi A., Memariani T., Yazdi M.V., Rezaee R., Calina D., Docea A.O., Goumenou M., Etemad L. Cytotoxic and apoptogenic properties of Dracocephalum kotschyi aerial part different fractions on calu-6 and mehr-80 lung cancer cell lines. Farmacia. 2017;65:189–199. [Google Scholar]
  • 30.Weng Y.P., Hung P.F., Ku W.Y., Chang C.Y., Wu B.H., Wu M.H., Yao J.Y., Yang J.R., Lee C.H. The inhibitory activity of gallic acid against DNA methylation: application of gallic acid on epigenetic therapy of human cancers. Oncotarget. 2018;9:361. doi: 10.18632/oncotarget.23015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 31.Shanmuganathan S., Angayarkanni N. Chebulagic acid chebulinic acid and gallic acid, the active principles of Triphala, inhibit TNFα induced pro-angiogenic and pro-inflammatory activities in retinal capillary endothelial cells by inhibiting p38, ERK and NF-kB phosphorylation. Vascul. Pharmacol. 2018;108:23–35. doi: 10.1016/j.vph.2018.04.005. [DOI] [PubMed] [Google Scholar]
  • 32.Sanlier N., Atik İ., Atik A. A minireview of effects of white tea consumption on diseases. Trends Food Sci. Technol. 2018;82:82–88. [Google Scholar]
  • 33.Singh J., Saha L., Singh N., Kumari P., Bhatia A., Chakrabarti A. Study of nuclear factor-2 erythroid-related factor-2 activator, berberine, in paclitaxel-induced peripheral neuropathy pain model in rats. J. Pharm. Pharmacol. 2019;71:797–805. doi: 10.1111/jphp.13047. [DOI] [PubMed] [Google Scholar]
  • 34.Verma S., Singh A., Fau - Mishra A., Mishra A. Gallic acid: molecular rival of cancer. Environ. Toxicol. Pharmacol. 2013;35:473–485. doi: 10.1016/j.etap.2013.02.011. [DOI] [PubMed] [Google Scholar]
  • 35.Teixeira J., Oliveira C., Cagide F., Amorim R., Garrido J., Borges F., Oliveira P.J. Discovery of a new mitochondria permeability transition pore (mPTP) inhibitor based on gallic acid. J. Enzyme Inhib. Med. Chem. 2018;33:567–576. doi: 10.1080/14756366.2018.1442831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 36.Rajalakshmi K., Devaraj S., Niranjali H.Fau–, Devaraj, Niranjali Devaraj S. Assessment of the no-observed-adverse-effect level (NOAEL) of gallic acid in mice. Food Chem. Toxicol. 2001;39:919–922. doi: 10.1016/s0278-6915(01)00022-9. [DOI] [PubMed] [Google Scholar]
  • 37.Hamada N.M., Ashour R.H., Shalaby A.A., El-Beltagi H.M. Calcitonin potentiates the anticonvulsant and antinociceptive effects of valproic acid and pregabalin in pentylenetetrazole-kindled mice. Eur. J. Pharmacol. 2018;818:351–355. doi: 10.1016/j.ejphar.2017.11.003. [DOI] [PubMed] [Google Scholar]
  • 38.Kaul I., Amin A., Rosenberg M., Rosenberg L., Meyer W.J., III Use of gabapentin and pregabalin for pruritus and neuropathic pain associated with major burn injury: a retrospective chart review. Burns. 2018;44:414–422. doi: 10.1016/j.burns.2017.07.018. [DOI] [PubMed] [Google Scholar]
  • 39.Masocha W. Paclitaxel-induced hyposensitivity to nociceptive chemical stimulation in mice can be prevented by treatment with minocycline. Sci. Rep. 2014;4:6719. doi: 10.1038/srep06719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 40.Choi Y., Yoon Y.W., Na H.S., Kim S.H., Chung J.M. Behavioral signs of ongoing pain and cold allodynia in a rat model of neuropathic pain. Pain. 1994;59:369–376. doi: 10.1016/0304-3959(94)90023-X. [DOI] [PubMed] [Google Scholar]
  • 41.Erichsen H.K., Blackburn-Munro G. Pharmacological characterisation of the spared nerve injury model of neuropathic pain. Pain. 2002;98:151–161. doi: 10.1016/s0304-3959(02)00039-8. [DOI] [PubMed] [Google Scholar]
  • 42.Hargreaves K., Dubner R., Brown F., Flores C., Joris J. A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia. Pain. 1988;32:77–88. doi: 10.1016/0304-3959(88)90026-7. [DOI] [PubMed] [Google Scholar]
  • 43.D’Amour F.E., Smith D.L. A method for determining loss of pain sensation. J. Pharmacol. Exp. Ther. 1941;72:74–79. [Google Scholar]
  • 44.Takagi H., Inukai T., Nakama M. A modification of Haffner’s method for testing analgesics. Jpn. J. Pharmacol. 1966;16:287–294. doi: 10.1254/jjp.16.287. [DOI] [PubMed] [Google Scholar]
  • 45.Moon S.H., Kim D., Shimizu N., Okada T., Hitoe S., Shimoda H. Ninety-day oral toxicity study of rice-derived gamma-oryzanol in Sprague-Dawley rats. Toxicol. Rep. 2017;4:9–18. doi: 10.1016/j.toxrep.2016.12.001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 46.Deshmukh N.S., Stohs S.J., Magar C.C., Kale A., Sowmya B. Bitter orange (Citrus aurantium L.) extract subchronic 90-day safety study in rats. Toxicol. Rep. 2017;4:598–613. doi: 10.1016/j.toxrep.2017.11.002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 47.Ohkawa H., Ohishi N., Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 1979;95:351–358. doi: 10.1016/0003-2697(79)90738-3. [DOI] [PubMed] [Google Scholar]
  • 48.Beutler E., Duron O., Kelly B.M. Improved method for the determination of blood glutathione. J. Lab. Clin. Med. 1963;61:882–888. PMID:13967893. [PubMed] [Google Scholar]
  • 49.Severinghaus J.W., Ferrebee J.W. Calcium determination by flame photometry; methods for serum, urine, and other fluids. J. Biol. Chem. 1950;187:621–630. PMID:14803444. [PubMed] [Google Scholar]
  • 50.Muthuraman A., Jaggi A.S., Singh N., Singh D. Ameliorative effects of amiloride and pralidoxime in chronic constriction injury and vincristine-induced painful neuropathy in rats. Eur. J. Pharmacol. 2008;587:104–111. doi: 10.1016/j.ejphar.2008.03.042. [DOI] [PubMed] [Google Scholar]
  • 51.Muthuraman A., Singh N., Jaggi A.S. Protective effect of Acorus calamus L. In rat model of vincristine-induced painful neuropathy: an evidence of anti-inflammatory and anti-oxidative activity. Food Chem. Toxicol. 2011;49:2557–2563. doi: 10.1016/j.fct.2011.06.069. [DOI] [PubMed] [Google Scholar]
  • 52.Wang H.D., Pagano P.J., Du Y., Cayatte A.J., Quinn M.T., Brecher P., Cohen R.A. Superoxide anion from the adventitia of the rat thoracic aorta inactivates nitric oxide. Circ. Res. 1998;82:810–818. doi: 10.1161/01.res.82.7.810. [DOI] [PubMed] [Google Scholar]
  • 53.Muthuraman A., Singh N. Neuroprotective effect of saponin-rich extract of Acorus calamus L. in rat model of chronic constriction injury (CCI) of sciatic nerve-induced neuropathic pain. J. Ethnopharmacol. 2012;142:723–731. doi: 10.1016/j.jep.2012.05.049. [DOI] [PubMed] [Google Scholar]
  • 54.Patriarca P., Dri P., Snidero M. Interference of myeloperoxidase with the estimation of superoxide dismutase activity. J. Lab. Clin. Med. 1977;90:289–294. PMID: 196030. [PubMed] [Google Scholar]
  • 55.Grisham M.B., Specian R.D., Zimmerman T.E. Effects of nitric oxide synthase inhibition on the pathophysiology observed in a model of chronic granulomatous colitis. J. Pharmacol. Exp. Ther. 1994;271:1114–1121. PMID: 7525937. [PubMed] [Google Scholar]
  • 56.Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951;193:265–275. PMID: 14907713. [PubMed] [Google Scholar]
  • 57.Li L., Li J., Zuo Y., Dang D., Frost J.A., Yang Q. Activation of KCNQ channels prevents paclitaxel-induced peripheral neuropathy and associated neuropathic pain. J. Pain. 2018;S1526-5900:30846–30850. doi: 10.1016/j.jpain.2018.11.001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 58.Brzeziński K. Chemotherapy-induced polyneuropathy. Part I Pathophysiol. Contemp. Oncol. 2012;16:72–78. doi: 10.5114/wo.2012.27341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 59.Yesil H., Eyigor S., Kayıkcıoglu M., Uslu R., Inbat M., Ozbay B. Is neuropathic pain associated with cardiac sympathovagal activity changes in patients with breast cancer? Neurol. Res. 2018;40:297–302. doi: 10.1080/01616412.2018.1438225. [DOI] [PubMed] [Google Scholar]
  • 60.Kober K.M., Mazor M., Abrams G., Olshen A., Conley Y.P., Hammer M., Schumacher M., Chesney M., Smoot B., Mastick J. Phenotypic characterization of paclitaxel-induced peripheral neuropathy in cancer survivors. J. Pain Symptom Manag. 2018;56:908–919. doi: 10.1016/j.jpainsymman.2018.08.017. e3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 61.Griffiths L.A., Duggett N.A., Pitcher A.L., Flatters S.J.L. Evoked and ongoing pain-like behaviours in a rat model of paclitaxel-induced peripheral neuropathy. Pain Res. Manag. 2018;2018 doi: 10.1155/2018/8217613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 62.Li Z., Zhao S., Zhang H.-L., Liu P., Liu F.-F., Guo Y.-X., Wang X.-L. Proinflammatory factors mediate paclitaxel-induced impairment of learning and memory. Mediators Inflamm. 2018;2018 doi: 10.1155/2018/3941840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 63.Snutch T.P., Zamponi G.W. Recent advances in the development of T-type calcium channel blockers for pain intervention. Br. J. Pharmacol. 2018;175:2375–2383. doi: 10.1111/bph.13906. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 64.Boyette-Davis J.A., Hou S., Abdi S., Dougherty P.M. An updated understanding of the mechanisms involved in chemotherapy-induced neuropathy. Pain Manag. 2018;8:363–375. doi: 10.2217/pmt-2018-0020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 65.Dinicola S., Fuso A., Cucina A., Santiago-Reyes M., Verna R., Unfer V., Monastra G., Bizzarri M. Natural products–alpha-lipoic acid and acetyl-L-carnitine–in the treatment of chemotherapy-induced peripheral neuropathy. Eur. Rev. Med. Pharmacol. Sci. 2018;22:4739–4754. doi: 10.26355/eurrev_201807_15534. [DOI] [PubMed] [Google Scholar]
  • 66.Han Y., Smith M.T. Pathobiology of cancer chemotherapy-induced peripheral neuropathy (CIPN) Front. Pharmacol. 2013;4:156. doi: 10.3389/fphar.2013.00156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 67.Fidanboylu M., Griffiths L.A., Flatters S.J.L. Global inhibition of reactive oxygen species (ROS) inhibits paclitaxel-induced painful peripheral neuropathy. PLoS One. 2011;6 doi: 10.1371/journal.pone.0025212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 68.Muthuraman A., Ramesh M. Ischemic-reperfusion of unilateral external iliac artery in rat: a new model for vasculitic femoral neuropathy. Neurosci. Lett. 2016;628:10–16. doi: 10.1016/j.neulet.2016.06.015. [DOI] [PubMed] [Google Scholar]
  • 69.Kaur P., Muthuraman A., Kaur J. Ameliorative potential of angiotensin-converting enzyme inhibitor (ramipril) on chronic constriction injury of sciatic nerve induced neuropathic pain in mice. J. Renin Angiotensin Aldosterone Syst. 2015;16:103–112. doi: 10.1177/1470320314556171. [DOI] [PubMed] [Google Scholar]
  • 70.Muthuraman A., Ramesh S., Fau - Sood M., Sood S. Ameliorative potential of montelukast on ischemia-reperfusion injury induced vasculitic neuropathic pain in rat. Life Sci. 2012;90:755–762. doi: 10.1016/j.lfs.2012.03.010. [DOI] [PubMed] [Google Scholar]
  • 71.Thiagarajan V.R., Shanmugam P., Krishnan U.M., Muthuraman A., Singh N. Ameliorative potential of Butea monosperma on chronic constriction injury of sciatic nerve induced neuropathic pain in rats. An. Acad. Bras. Cienc. 2012;84:1091–1104. doi: 10.1590/s0001-37652012005000063. [DOI] [PubMed] [Google Scholar]
  • 72.Vanitha S., Thiagarajan V.R.K., Muthuraman A., Krishnan S., Aruna A., Tharabai R. Pharmacological evaluation of methanolic leaf extract of Swietenia mahagoni on acrylamide-induced neuropathic pain in rats. Toxicol. Ind. Health. 2015;31:1185–1194. doi: 10.1177/0748233713491808. [DOI] [PubMed] [Google Scholar]
  • 73.Muthuraman A., Diwan V., Jaggi A.S., Singh N., Singh D. Ameliorative effects of Ocimum sanctum in sciatic nerve transection-induced neuropathy in rats. J. Ethnopharmacol. 2008;120:56–62. doi: 10.1016/j.jep.2008.07.049. [DOI] [PubMed] [Google Scholar]
  • 74.Thiagarajan V.R., Shanmugam P., Krishnan U.M., Muthuraman A. Ameliorative potential of Vernonia cinerea on chronic constriction injury of sciatic nerve induced neuropathic pain in rats. An. Acad. Bras. Cienc. 2014;86:1435–1450. doi: 10.1590/0001-3765201420130404. [DOI] [PubMed] [Google Scholar]
  • 75.Xie H.T., Xia Z.Y., Pan X., Zhao B., Liu Z.G. Puerarin ameliorates allodynia and hyperalgesia in rats with peripheral nerve injury. Neural Regen. Res. 2018;13:1263–1268. doi: 10.4103/1673-5374.235074. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 76.Xie M.X., Zhu H.Q., Pang R.P., Wen B.T., Liu X.G. Mechanisms for therapeutic effect of bulleyaconitine A on chronic pain. Mol. Pain. 2018;14 doi: 10.1177/1744806918797243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 77.Kim S.H., Jun Cd Fau - Suk K., Suk K Fau - Choi B.-J., Choi BjFau - Lim H., Lim H Fau - Park S., Park S Fau - Lee S.H., Lee ShFau - Shin H.-Y., Shin HyFau - Kim D.-K., Kim DkFau - Shin T.-Y., Shin T.Y. Gallic acid inhibits histamine release and pro-inflammatory cytokine production in mast cells. Toxicol. Sci. 2006;91:123–131. doi: 10.1093/toxsci/kfj063. [DOI] [PubMed] [Google Scholar]
  • 78.Kim M.J., SeongArFau - Yoo J.-Y., YooJyFau - Jin C.-H., JinChFau - Lee Y.-H., Lee YhFau - Kim Y.J., Kim YjFau - Lee J., Lee J Fau - Jun W.J., Jun WjFau - Yoon H.-G., Yoon H.G. Gallic acid, a histone acetyltransferase inhibitor, suppresses beta-amyloid neurotoxicity by inhibiting microglial-mediated neuroinflammation. Mol. Nutr. Food Res. 2011;55:1798–1808. doi: 10.1002/mnfr.201100262. [DOI] [PubMed] [Google Scholar]
  • 79.Maya S., Prakash T., Goli D. Evaluation of neuroprotective effects of wedelolactone and gallic acid on aluminium-induced neurodegeneration: relevance to sporadic amyotrophic lateral sclerosis. Eur. J. Pharmacol. 2018;835:41–51. doi: 10.1016/j.ejphar.2018.07.058. [DOI] [PubMed] [Google Scholar]
  • 80.Nussbaum L., Hogea L.M., Călina D., Andreescu N., Grădinaru R., Ștefănescu R., Puiu M. Modern treatment approaches in psychoses. Pharmacogenetic, neuroimagistic and clinical implications. Farmacia. 2017;65:75–81. [Google Scholar]
  • 81.Devasagayam T.P., Boloor K.K., Ramasarma T. Methods for estimating lipid peroxidation: an analysis of merits and demerits. Indian J. Biochem. Biophys. 2003;40:300–308. http://hdl.handle.net/123456789/3805 [PubMed] [Google Scholar]
  • 82.Zeb A., Ullah F. A simple spectrophotometric method for the determination of thiobarbituric acid reactive substances in fried fast foods. J. Anal. Methods Chem. 2016;2016 doi: 10.1155/2016/9412767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 83.Chen D., Zhang G., Li R., Guan M., Wang X., Zou T., Zhang Y., Wang C.-R., Shu C., Hong H. Biodegradable, hydrogen peroxide and glutathione dual responsive nanoparticles for potential programmable paclitaxel release. J. Am. Chem. Soc. 2018;140:7373–7376. doi: 10.1021/jacs.7b12025. [DOI] [PubMed] [Google Scholar]
  • 84.Waseem M., Kaushik P., Tabassum H., Parvez S. Role of mitochondrial mechanism in chemotherapy-induced peripheral neuropathy. Curr. Drug Metab. 2018;19:47–54. doi: 10.2174/1389200219666171207121313. [DOI] [PubMed] [Google Scholar]
  • 85.Xu J., Zhang L., Xie M., Li Y., Huang P., Saunders T.L., Fox D.A., Rosenquist R., Lin F. Role of complement in a rat model of paclitaxel-induced peripheral neuropathy. J. Immunol. 2018;200:4094–4101. doi: 10.4049/jimmunol.1701716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 86.Wu B.-Y., Liu C.-T., Su Y.-L., Chen S.-Y., Chen Y.-H., Tsai M.-Y. A review of complementary therapies with medicinal plants for chemotherapy-induced peripheral neuropathy. Complement. Ther. Med. 2019;42:226–232. doi: 10.1016/j.ctim.2018.11.022. [DOI] [PubMed] [Google Scholar]

Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Transparency document
mmc1.zip (2.7MB, zip)

Articles from Toxicology Reports are provided here courtesy of Elsevier

RESOURCES