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. 2019 May 17;8(5):475. doi: 10.3390/cells8050475

Figure 2.

Figure 2

Subcellular localization of eGFP-Beclin 1-ER and eGFP-Beclin 1-MITO, as well as the corresponding targeted control constructs, in mouse embryonic fibroblasts-wild type (MEF-WT). (A) MEF-WT cells were transfected with eGFP-Beclin 1-ER or eGFP-ER as indicated, and labelled against endoplasmic reticulum (ER) marker BAP31. (B) MEF-WT cells were transfected with eGFP-Beclin 1-MITO and eGFP-MITO as indicated, and labelled against mitochondrial marker TOM20. The arrows indicate one of the structures showing colocalization. Images were taken with a confocal microscope and one optical section is shown. Asterisks indicate cells expressing the constructs. Scale bars, 10 μm.