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. 2019 May 10;11(5):430. doi: 10.3390/v11050430

Figure 3.

Figure 3

DNA accessibility and capsid integrity of the different B19V density populations. (A,B) Nuclease sensitivity of viruses in HEPES or in PBS displaying different densities. Virus samples from representative density populations were treated with DNase I at 37 °C for 1 h (white columns). Virus treated at 80 °C for 5 min was used as control. (C,D) Integrity of capsids in HEPES or in PBS displaying different densities. Virus samples from representative density populations were immunoprecipitated with the conformational antibody 860-55D against assembled capsids. Virus treated at 80 °C for 5 min was used as control. (E) Transmission electron microscopy (TEM) images of B19V in PBS alone and PBS supplemented with 1 mM MgCl2 and exposed to a freeze/thaw cycle. Arrows indicate capsids with rearranged DNA. (F) Infectivity assay based on NS1 mRNA detection with the same B19V preparations used for TEM. SE; short exposure. F/T; freeze and thaw. Values correspond to genome equivalents per microliter (geq/µL).